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系統識別號 U0026-2908201612342900
論文名稱(中文) 比較樹脂浸潤與牙科窩溝封填劑在牛牙上的機械性能:體外實驗
論文名稱(英文) Comparing the mechanical properties between the resin infiltrant and dental sealants on the bovine incisors: in-vitro study
校院名稱 成功大學
系所名稱(中) 口腔醫學研究所
系所名稱(英) Institute of Oral Medicine
學年度 104
學期 2
出版年 105
研究生(中文) 丁可欣
研究生(英文) Ke-Sin Ding
學號 T46034078
學位類別 碩士
語文別 中文
論文頁數 54頁
口試委員 指導教授-黃振勳
口試委員-謝達斌
口試委員-陳弘森
中文關鍵字 早期齲齒病灶  樹脂浸潤  窩溝封填劑  表面粗糙度  奈米壓痕 
英文關鍵字 early caries lesion  resin infiltration  sealant  surface roughness  nanoindentation 
學科別分類
中文摘要 早期齲齒病灶主要位於牙釉質,通常出現在牙齒之唇側面或鄰接面。針對早期齲齒病灶,目前被認為有效的表面處理方法為樹脂浸潤,市售商品名為Icon infiltrant,其宣稱可減緩早期齲齒病灶甚至終止病程。
關於應用Icon infiltrant於鄰接面齲齒的體內研究,普遍認為有良好的結果;於體外實驗則顯示Icon infiltrant有良好的滲透力,甚至對牙齒表面的硬度有正向幫助。
但也有研究指出窩溝封填劑比Icon infiltrant有較低的溶解度,以及被Icon infiltrant處理過的牙釉質仍需擔心染上污漬的問題。然而,目前只有少數文獻提及被Icon infiltrant處理過的牙齒表面於微觀下會有如何的材料表現。本研究之目的是比較樹脂浸潤材料Icon infiltrant與窩溝封填劑應用在人造早期齲齒病灶後的機械性質。
本研究屬於體外實驗,以牛門牙進行3天的酸鹼循環 (pH值為5.0)後製成人造早期齲齒病灶(early caries lesion),再以樹脂浸潤材料(Icon infiltrant,Icon組)以及窩溝封填劑(UltraSeal XT® hydro,Seal組) 分別進行處理後,一半樣本浸泡於去離子水3天(Icon-T及Seal-T組),另一半樣本則再次進行3天的酸鹼循環(Icon-R及Seal-R組)。之後以掃描式電子顯微鏡、能量散射光譜儀、表面粗度儀與奈米壓痕觀察樣本,比較兩種材料在再次酸鹼循環前後機械性質之差異。
本研究之結論為牛門牙早期齲齒病灶之表面處理,樹脂浸潤材料較窩溝封填劑來的粗糙,在奈米硬度方面,樹脂浸潤材料也較弱;若在材料皆未至牙齒表面脫落的情況下,兩者抗酸能力則無差異。
英文摘要 Early caries lesions are mainly located on labial and/or proximal surfaces of enamel. The most referred method of surface management is resin infiltration such as the Icon infiltrant. It was claimed to have the effects of decreasing or even eliminating the process of early caries lesion.
According to the previous studies, it is generally accepted that proximal caries could be treated with Icon infiltrant as an effective method in vivo. Other in vitro studies indicated that Icon infiltrant has a preferable penetration capability, and even showed the positive effect to the hardness of surface.
However, other study revealed that the solubility of sealants is lower than that of Icon infiltrant. In vitro studies also revealed that enamel treated with Icon infiltrant could be susceptible to staining. Moreover, the main content of Icon infiltrant is resin matrix, very few studies referred to the performance of Icon infiltrant at microscopic level. The aims of this study were to compare the mechanical properties between the resin infiltration material and the dental sealants on the artificial early caries lesions.
In this in-vitro study, we created artificial early caries lesions over bovine incisors by pH cycling (pH 5.0). The early caries lesions were treated with the resin infiltration material (Icon infiltrant, Icon group), or the fissure sealant (UltraSeal XT® hydro, Seal group). Half of Icon group and half of Seal group (Icon-T and Seal-T) placed in deionized water for 3 days, and the rest samples (Icon-R and Seal-R) were subjected to the second pH cycling (the second acid challenge). Scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), the surface profilometer and the nanoindentor were conducted to compare the mechanical properties and topography of these two materials before and after the second acid challenge.
The results of this study revealed the surface roughness of the resin infiltration being higher than the sealants but the nanohardeness of the resin infiltration being lower than the sealants. Both materials have the similar acid resistance under the 2nd acidic challenge.
論文目次 中文摘要 I
Abstract III
誌謝 VIII
目錄 IX
表目錄 XI
圖目錄 XII
第一章 序論 (Introduction) 1
1.1. 牙釉質與早期齲齒病灶 (Enamel and early caries lesions) 1
1.2. 微侵襲治療的研究回顧(Review of microinvasive intervention) 4
1.3. 以牛牙作為人牙替代物 (Bovine teeth as substitute for human teeth) 6
1.4. 酸鹼循環的回顧 (Review of pH cycling) 6
1.5. 奈米壓痕測試 (Nanoindentation test) 8
1.6 動機與目標 (Motivation and purposes) 9
第二章 材料與方法 (Materials and methods) 10
2.1. 牛牙樣品備製 (Sample preparation of bovine teeth) 11
2.2. 人造早期齲齒病灶備製(Preparation of artificial early caries lesion) 13
2.3. 操作樹脂浸潤材料與窩溝封填劑 (Icon and sealant application) 15
2.4. 掃描式電子顯微鏡/能量散佈光譜儀(SEM/EDS, Scanning Electron Microscope / Energy Dispersive Spectrometer) 16
2.5. 表面粗糙度計算 (Measurements of surface roughness) 17
2.6. 奈米壓痕計算 (Nanoindentation measurements) 18
2.7. 統計分析 (Statistical analysis) 19
第三章 結果 (Results) 20
3.1. 各組影像紀錄 (Results of images) 20
3.2. 表面形貌 (Surface topograph) 22
3.3. 比較表面粗糙度 (Comparison of surface roughness) 34
3.4. 比較奈米硬度(H) (Comparison of nanohardness(H)) 37
第四章 討論 (Discussion) 45
第五章 結論 (Conclusion) 50
參考文獻 (References) 51
參考文獻 [1] Xu C, Yao X, Walker MP, Wang Y. Chemical/molecular structure of the dentin-enamel junction is dependent on the intratooth location. Calcified tissue international 2009;84:221-8.
[2] Besinis A, van Noort R, Martin N. Remineralization potential of fully demineralized dentin infiltrated with silica and hydroxyapatite nanoparticles. Dental materials 2014;30:249-62.
[3] White DJ. The application of in vitro models to research on demineralization and remineralization of the teeth. Advances in dental research 1995;9:175-93.
[4] Roberson TM, Lundeen T. Cariology: The lesion, etiology, prevention and control. Sturdevant’s Art and Sience of Operative Dentistry, 4th ed. Mosby 2002:98-129.
[5] Srivastava K, Tikku T, Khanna R, Sachan K. Risk factors and management of white spot lesions in orthodontics. Journal of orthodontic science 2013;2:43-9.
[6] Paris S, Meyer-Lueckel H. Inhibition of caries progression by resin infiltration in situ. Caries research 2010;44:47-54.
[7] Tyas MJ, Anusavice KJ, Frencken JE, Mount GJ. Minimal intervention dentistry--a review. FDI Commission Project 1-97. International dental journal 2000;50:1-12.
[8] Vila Verde A, Ramos MM, Stoneham AM. Benefits in cost and reduced discomfort of new techniques of minimally invasive cavity treatment. Journal of dental research 2009;88:297-9.
[9] Paris S, Schwendicke F, Seddig S, Muller WD, Dorfer C, Meyer-Lueckel H. Micro-hardness and mineral loss of enamel lesions after infiltration with various resins: Influence of infiltrant composition and application frequency in vitro. Journal of dentistry 2013;41:543-8.
[10] Waggoner WF, Johnston WM, Schumann S, Schikowski E. Microabrasion of human enamel in vitro using hydrochloric acid and pumice. Pediatric dentistry 1989;11:319-23.
[11] Akin M, Basciftci FA. Can white spot lesions be treated effectively? Angle orthodontist 2012;82:770-5.
[12] Gray GB, Shellis P. Infiltration of resin into white spot caries-like lesions of enamel: an in vitro study. The European journal of prosthodontics and restorative dentistry 2002;10:27-32.
[13] Bidarkar A. In vitro prevention of secondary demineralization by icon (infiltration concept). MS (Master of Science) thesis, University of Iowa, 2011.
[14] Robinson C, Hallsworth AS, Weatherell JA, Kunzel W. Arrest and control of carious lesions: a study based on preliminary experiments with resorcinol-formaldehyde resin. Journal of dental research 1976;55:812-8.
[15] Robinson C, Brookes SJ, Kirkham J, Wood SR, Shore RC. In vitro studies of the penetration of adhesive resins into artificial caries-like lesions. Caries research 2001;35:136-41.
[16] Luebbers D, Spieler-Husfeld K, Staude C. In vitro color stability of infiltrated carious lesions. 2009, Data on file. DMG, Hamburg,Germany. http://drilling-no-thanks.info/downloads/Icon_Science_Book_3rd%20 Edition.pdf.
[17] Dorri M, Dunne SM, Walsh T, Schwendicke F. Micro-invasive interventions for managing proximal dental decay in primary and permanent teeth. Cochrane database of systematic reviews 2015.
[18] Martignon S, Ekstrand KR, Gomez J, Lara JS, Cortes A. infiltrating/sealing proximal caries lesions: A 3-year randomized clinical trial. Journal of dental research 2012;91:288-92.
[19] Gelani R, Zandona AF, Lippert F, Kamocka MM, Eckert G. In vitro progression of artificial white spot lesions sealed with an infiltrant resin. Operative dentistry 2014;39:481-8.
[20] Oliveira GC, Boteon AP, Ionta FQ, Moretto MJ, Honorio HM, Wang L, Rios D. In vitro effects of resin infiltration on enamel erosion inhibition. Operative dentistry 2015;40:492-502.
[21] Horuztepe SA, Firat E, Gurgan S, Onen A, Abaci S. Preventing capability of resin-infiltration-technique on occlusal fissures. Conference paper 2010.
[22] Taher NM, Alkhamis HA, Dowaidi SM. The influence of resin infiltration system on enamel microhardness and surface roughness: An in vitro study. Saudi dental journal 2012; 24:79–84.
[23] Taher NM. Atomic force microscopy and tridimensional topography analysis of human enamel after resinous infiltration and storage in water. Saudi medical journal 2013;34:408-14.
[24] Borges A, Caneppele T, Luz M, Pucci C, Torres C. Color stability of resin used for caries infiltration after exposure to different staining solutions. Operative dentistry 2014;39:433-40.
[25] Araujo GS, Naufel FS, Alonso RC, Lima DA, Puppin-Rontani RM. Influence of staining solution and bleaching on color stability of resin used for caries infiltration. Operative dentistry 2015;40:E250-6.
[26] Yassen GH, Platt JA, Hara AT. Bovine teeth as substitute for human teeth in dental research: a review of literature. Journal of oral science 2011;53:273-82.
[27] Lippert F, Juthani K. Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions. Clinical oral investigations 2015;19:1947-54.
[28] Cummins. Working group report 3: Role of models in assessing new agents for caries prevention. Advances in dental research 1995;9:338-9.
[29] Buzalaf MA, Hannas AR, Magalhaes AC, Rios D, Honorio HM, Delbem AC. pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations. Journal of applied oral science 2010;18:316-34.
[30] Maia LC, de Souza IP, Cury JA. Effect of a combination of fluoride dentifrice and varnish on enamel surface rehardening and fluoride uptake in vitro. European journal of oral sciences 2003;111:68-72.
[31] Page DJ. A study of the effect of fluoride delivered from solution and dentifrices on enamel demineralization. Caries research 1991;25:251-5.
[32] ten Cate JM. The caries preventive effect of a fluoride dentifrice containing Triclosan and zinc citrate, a compilation of in vitro and in situ studies. International dental journal 1993;43:407-13.
[33] ten Cate JM, Buijs MJ, Damen JJ. pH-cycling of enamel and dentin lesions in the presence of low concentrations of fluoride. European journal of oral sciences 1995;103:362-7.
[34] ten Cate JM, Exterkate RA, Buijs MJ. The relative efficacy of fluoride toothpastes assessed with pH cycling. Caries research 2006;40:136-41.
[35] Brighenti FL, Delbem AC, Buzalaf MA, Oliveira FA, Ribeiro DB, Sassaki KT. In vitro evaluation of acidified toothpastes with low fluoride content. Caries research 2006;40:239-44.
[36] Alves KM, Pessan JP, Brighenti FL, Franco KS, Oliveira FA, Buzalaf MA, Sassaki KT, Delbem AC. In vitro evaluation of the effectiveness of acidic fluoride dentifrices. Caries research 2007;41:263-7.
[37] Buchalla W, Imfeld T, Attin T, Swain MV, Schmidlin PR. Relationship between nanohardness and mineral content of artificial carious enamel lesions. Caries research 2008;42:157-63.
[38] Iijima M, Muguruma T, Brantley WA, Ito S, Yuasa T, Saito T, Mizoguchi I. Effect of bracket bonding on nanomechanical properties of enamel. American journal of orthodontics and dentofacial orthopedics 2010; 138:735-40.
[39] Tostes MA, Santos E Jr, Camargo SA Jr. Effect of resin infiltration on the nanomechanical properties of demineralized bovine enamel. Indian journal of dentistry 2014;5:116-22.
[40] ten Cate JM, Buijs MJ, Damen JJ. pH‐cycling of enamel and dentin lesions in the presence of low concentrations of fluoride. European journal of oral sciences 1995;103:362-7.
[41] Oliver WC, Pharr GM. An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments. Journal of materials research 1992;7:1564-83.
[42] Jeng YR, Lin TT, Huang JS, Peng SR, Shieh DB. Topical laser application enhances enamel fluoride uptake and tribological properties. Journal of dental research 2013;92:165-60.
[43] Guclu ZA, Donmez N, Tuzuner T, Odabas ME, Hurt AP, Coleman NJ. The impact of Er:YAG laser enamel conditioning on the microleakage of a new hydrophilic sealant-UltraSeal XT (R) hydro (TM). Lasers in medical science 2016;31:705-11.
[44] Yang Y, Lv XP, Shi W, Li JY, Li DX, Zhou XD, Zhang LL. 8DSS-promoted remineralization of initial enamel caries in vitro. Journal of dental research 2014;93:520-4.
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