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系統識別號 U0026-2706201011175600
論文名稱(中文) 卡路里限制下的基因表現及其代謝路徑的探討
論文名稱(英文) Gene Expression in Caloric Restriction and Pathway Analysis
校院名稱 成功大學
系所名稱(中) 統計學系碩博士班
系所名稱(英) Department of Statistics
學年度 98
學期 2
出版年 99
研究生(中文) 曾怡靜
研究生(英文) Yi-Jing Tzseng
學號 r2697107
學位類別 碩士
語文別 中文
論文頁數 23頁
口試委員 口試委員-李隆安
口試委員-陳君厚
口試委員-辛致煒
口試委員-鄧致剛
指導教授-詹世煌
中文關鍵字 多時期互補核酸微陣列晶片  蛋白激酶  卡路里限制法  轉折時間點  代謝路徑 
英文關鍵字 time-course cDNA microarray data  protein kinase  gene expression  calorie restriction  change time point  pathway 
學科別分類
中文摘要 大部分的生物現象或疾病表現通常都是由許多基因一起發揮作用產生的結果。近年來,科學家發現卡路里限制法(Caloric restriction)可使生物延長壽命,且與蛋白激酶的調控機制有關。 王佩力(2009)分析多時期互補核酸微陣列晶片(cDNA microarray),篩選出210條蛋白激酶。本文進一步分析此210條蛋白激酶之表現行為。以無母數迴歸之lowess法概估基因表現,之後就此lowess估計值利用piecewise線性模型來估計轉折時間點和基因表現速率後分群,挑選出32個感興趣的基因。進一步分析,發現其中有4個基因,其Locus分別為TVAG_089630、TVAG_339340、 TVAG_089630與 TVAG_339340,並經實驗驗證其表現(王儷霖,2010),此4個基因經統計方法驗證其顯著性。由於此等基因和MAPK family pathway及AMPK Enzyme Complex pathway有關,對基因間互動行為的探討或許有助於我們對長壽基因的了解。
英文摘要 Most of the biological phenomena or disease is the result of the interactions of multiple genes. Extensive studies on the restriction of caloric (CR) intake showed that CR can extend the longevity in many organisms. Wang (2009) examined the expression level of 210 T.vaginals protein kinase genes under CR by using time-course cDNA microarray. In this thesis, we smoothed the gene expression of the 210 protein kinase, then used piecewise linear model to estimate the changing time that gene expression is dramatically different from the previous behavior, and the rate of gene expression. Applying complete linkage clustering method, thirty-two genes are found to have large changing point or with increasing pattern in gene expression after changing point. Statistical analysis shows that those patterns are highly significant, and four out of these thirty-two genes, TVAG_089630, TVAG_339340,TVAG_123500 and TVAG_456650,have been verified (Wang, 2010). The association of the found 32 genes is to be studied further, hoping that the interaction behaviors of these significant genes can be understood.


論文目次 第一章 緒論 ...1
第二章 有意義基因集的搜尋 2
2.1. 文獻探討 2
2.2. 尋找相關性較高之基因集的方法 3
第三章 陰道鞭毛蟲的長壽基因 4
3.1. 多時期微陣列基因晶片資料 4
3.2. 基因表現行為的模型配適 6
3.3. 長壽基因的選取 7
3.4. 基因集之信賴水準和可接受域 10
3.5. 長壽基因的選取 15
3.6. 基因集之顯著關聯性分析 17
3.7. 基因所屬的代謝路徑 18
第四章 結論 19
參考文獻 20
附錄 22
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15. Tian, L. et al. (2005), Discovering statistically significant pathways in expression profiling studies, Proceedings National Academy of Science, 102, 13544–13549.

16. Vergenes, B. et al. (2002), Cytoplasmic SIR2 jomologue overexpression promotes survival of Leishmania parasites by preventing programmed cell death. Gene, 296, 139-150.
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表達,台北醫學大學,碩士論文。
19. 王儷霖(2010),探討陰道鞭毛蟲蛋白激酶調控延長壽命的機轉,長庚大學,碩
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