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論文名稱(中文) 雌二醇透過活化BDNF-TrkB下游訊息分子PLCγ, PKC, PI3K 和MAPK來增強卵巢切除後之大白鼠杏仁體側核的長期增益現象
論文名稱(英文) 17β-Estradiol Acts through BDNF-TrkB Downstream Signaling Molecules PLCγ, PKC, PI3K and MAPK to Enhance Long-Term Potentiation in the Lateral Amygdala of Ovariectomized Rats
校院名稱 成功大學
系所名稱(中) 生理學研究所
系所名稱(英) Department of Physiology
學年度 104
學期 2
出版年 105
研究生(中文) 李維儒
研究生(英文) Wei-Ju Lee
學號 s36031085
學位類別 碩士
語文別 英文
論文頁數 49頁
口試委員 指導教授-吳豐森
召集委員-莊季瑛
口試委員-黃阿敏
中文關鍵字 腦源性神經滋養因子  雌二醇  杏仁體側核  長期增益現象  絲裂原活化蛋白質激酶  卵巢切除  磷酸肌醇3-激酶  磷酸激酶C  磷脂酶Cγ  TrkB受體 
英文關鍵字 BDNF  17β-Estradiol  Lateral amygdala  Long-term potentiation  MAPK  Ovariectomy  PI3K  PKC  PLCγ  TrkB receptor 
學科別分類
中文摘要 長期增益現象(LTP)是當突觸前神經纖維受到短暫高頻電刺激後,突觸間興奮性訊息傳遞持續性增強的一種現象。杏仁體側核所誘發的LTP(LA-LTP)已被指出是恐懼記憶形成的一種細胞模式。我們實驗室先前的研究發現,相較於雄性大鼠,雌性大鼠的LA-LTP顯著較大。此外,雌性類固醇激素雌二醇有參與在雌鼠LA-LTP比較大的現象中。最近的研究指出,成年母鼠給予雌二醇可透過活化腦源性神經滋養因子(BDNF)及其受體TrkB訊息路徑來增強海馬迴的LTP。然而,雌二醇是否亦能透過活化BDNF-TrkB訊息路徑來增強LA-LTP,則不得而知。因此在本研究中,我們使用胞外電生理記錄技術以及藥理學的方法,來探討BDNF-TrkB訊息路徑在雌二醇增強卵巢切除後之大鼠腦薄片LA-ATP的作用中所扮演的角色。我們的結果顯示,給予雌二醇的卵巢切除後之雌鼠腦薄片高頻電刺激所誘發的LA-LTP顯著被增強,確認我們先前的發現:補充給予雌二醇可增強卵巢切除後之雌鼠的LA-LTP。此外,在卵巢切除的雌鼠腦薄片中同時給予雌二醇與選擇性的TrkB受體拮抗劑ANA-12,可完全抑制雌二醇對LA-LTP所誘發的增強作用,暗示BDNF-TrkB訊息路徑介入了雌二醇所造成LA-LAP的增強作用。此外,分別給予磷脂酶C(PLC)抑制劑U73122、磷酸激酶C(PKC)抑制劑Bis I、磷酸肌醇3-激酶(PI3K)抑制劑wortmannin和MEK抑制劑PD98059,都可阻斷雌二醇對LA-LTP的增強作用,顯示雌二醇係透過活化BDNF-TrkB下游這些訊息傳遞分子來促進LA-LTP。綜合上述,我們的結果顯示,雌二醇透過活化BDNF-TrkB訊息路徑及其下游訊息傳遞分子包括PLC、PKC、PI3K與MAPK來增強卵巢切除後之雌鼠的LA-LTP。
英文摘要 Long-term potentiation (LTP) is a phenomenon in which brief high-frequency stimulation (HFS) of a synaptic pathway results in long-term enhancement of the efficacy of connections made by that pathway. LTP in the lateral nucleus of amygdala (LA) has been shown to be intimately involved in the cellular changes that underlie fear memory formation. Previous studies from our laboratory have demonstrated that LA-LTP is significantly greater in female rats than in male rats. In addition, the female sex steroid 17β-estradiol(E2) mediates female’s enhanced LA-LTP. Recent data indicate that E2 enhances hippocampal LTP via the brain-derived neurotrophic factor (BDNF)-tropomyosin-related kinase B (TrkB) pathway. However, whether the BDNF-TrkB pathway is also involved in E2-induced enhancement of LA-LTP remains unclear. In the present study, we thus used the extracellular electrophysiological recording technique and pharmacological methods to determine the role of the BDNF-TrkB pathway in E2-induced enhancement of LA-LTP in ovariectomized (OVX) female rat slices. Results revealed that LA-LTP in E2-treated OVX rat slices measured 40 min after HFS was significantly greater than that observed in vehicle-treated OVX rat slices, confirming our pervious finding that E2 replacement potentiated LA-LTP in OVX rat slices. In addition, bath perfusion of the selective TrkB antagonist ANA-12 abolished E2-induced enhancement of LA-LTP, suggesting the involvement of the BDNF-TrkB pathway in the enhancing effect of E2. Furthermore, bath perfusion of the PLCγ inhibitor U73122, the PKC inhibitor Bis I, the PI3K inhibitor wortmannin or the MEK inhibitor PD98059 completely blocked E2-induced enhancement of LA-LTP in the OVX rat slices, indicating that the enhancing effect of E2 was mediated by these BDNF-TrkB downstream signaling molecules. In summary, our results demonstrate that E2 acts through the BDNF-TrkB pathway and its downstream signaling molecules including PLCγ, PKC, PI3K and MAPK to enhance LA-LTP in OVX rats.
論文目次 Abstract in Chinese……………………………………………………...I
Abstract…...…………………………………………………………...III
Acknowledgement………...………………………...……………...…...V
Table of Contents………...…………….……………………...…...VI
List of Figures................................................................................IX
Abbreviations……………………………….………………………...XI
Introduction………………………………...…………………………...1
Amygdala, long-term potentiation, and fear memory………………………………1
Sex differences in hippocampal LTP and hippocampus-dependent learning and memory………………………………………………………………………………........3
Role of brain-derived neurotrophic factor (BDNF) in synaptic plasticity and memory…………………………………………………………………………………….5
Effects of sex steroids on BDNF expression levels………………………………...8
Rationale and the specific aim of this study…………………………………....8
Materials and Methods………………………….…………………….10
Animals………………………………………………………………………….10
Bilateral ovariectomy (OVX)….………………………………………...….10
Slice preparation…………………………………………………………...…...10
Extracellular electrophysiological recordings………………………………….11
Drugs and chemicals……………………………………………………………12
Statistical analysis……………………………………..…………………..12
Experimental design…………………………………………………………….12
Results…………………………………………………………….…….16
E2 enhances HFS-induced LA-LTP in OVX rat slices……………………………...16
Bath perfusion of ANA-12 abolishes E2-induced enhancement of LA-LTP in OVX rat slices………………………………………………………………………………….16
Bath perfusion of U73122 abolishes E2-induced enhancement of LA-LTP in OVX rat slices………………………………………………………………………………….17
Bath perfusion of Bis I abolishes E2-induced enhancement of LA-LTP in OVX rat slices………………………………………………………………………………………17
Bath perfusion of wortmannin abolishes E2-induced enhancement of LA-LTP in OVX rat slices…………………………………………………………………………...18
Bath perfusion of PD98059 abolishes E2-induced enhancement of LA-LTP in OVX rat slice…………………………………………………………………………….18
Discussion…………………………..…………………………………..20
Mechanisms underlying E2-induced activation of BDNF-TrkB in the LA are similar to those observed in the hippocampal CA1 area……………………..20
Role of PLCγ and PKC in E2-induced enhancement of LA-LTP………………….21
Role of PI3K in E2-induced enhancement of LA-LTP…………………………...21
Role of MAPK in E2-induced enhancement of LA-LTP………………....…………22
Activation of the BDNF-TrkB pathway is sufficient to enhance amygdala or hippocampal LTP……..…………………………………………………….............22
Physiology significance of our findings……………………………………………...23
Conclusion……………………...……………...……………………..23
References……………………....………………………………….…36
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