系統識別號 U0026-2408201316441200
論文名稱(中文) 甲硫胺酸限制飲食與耐力運動對去卵巢大鼠骨骼之代謝、組織型態與生物力學特性的影響
論文名稱(英文) The Effects of Methionine Restriction Diets and Endurance Exercise on Metabolism, Histomorphometry and Biomaterial Properties in Bone of Ovariectomized Rats
校院名稱 成功大學
系所名稱(中) 體育健康與休閒研究所
系所名稱(英) Institute of Physical Education, Health & Leisure Studies
學年度 101
學期 2
出版年 102
研究生(中文) 蘇亦秀
研究生(英文) I-Hsiu Su
學號 rb6001011
學位類別 碩士
語文別 英文
論文頁數 39頁
口試委員 指導教授-黃滄海
中文關鍵字 甲硫胺酸限制  耐力運動  骨代謝  骨質疏鬆 
英文關鍵字 Methionine restriction  Endurance exercise  Bone metabolism  Osteoporosis 
中文摘要 目的:本研究探討以甲硫胺酸限制飲食以及耐力運動之介入對切除卵巢大鼠骨骼之代謝、組織型態及生物力學特性的影響。
方法:週齡 35週之雌性Sprague-Dawley (SD) 大鼠分為六組,依照有無去除卵巢 (ovariectomy, Ovx)、飲食中甲硫胺酸 (methionine, M) 兩種含量正常 (0.86%) 和甲硫胺酸限制 (0.172%, MR)、運動訓練 (exercise, Exe) 和雌激素施打 (estrogen, Est) 等介入,將實驗動物分成Sham (偽手術組)、Ovx、Ovx+Exe、Ovx+MR、Ovx+MR+Exe和Ovx+Est 等六組。耐力運動訓練內容為16公尺/分鐘、1小時/天和5天/週 (為期12週) 之跑步訓練,於去除卵巢前兩週開始介入。甲硫胺酸限制飲食在去除卵巢後一週介入,採取自由取食之方式餵食,每週記錄兩天動物之體重與飲食飲水量。動物犧牲後所得之血液與骨骼樣本,分別進行血清骨代謝指標、骨組織型態分析、動態組織學分析和骨組織生物力學特性等變項分析。統計方法以單因子變異數分析 (one-way ANOVA) 或單因子共變數分析 (one-way ANCOVA) 進行組間各變項之考驗,當p< .05時,以LSD方法進行組間事後比較。
英文摘要 Purpose: To investigate the effects of methionine-restricted diets and endurance exercise on metabolism, histomorphometry and biomaterial properties in bone of ovariectomized rats.
Methods: Females Sprague-Dawley (SD) rats (35-week-old) were body weight matched and assigned to six groups according to methionine (M) content (e.g. 0.86% or 0.17%) in diet, sham or ovariectomy (Ovx) surgery, exercise (Exe) training and estrogen (Est) injection, which were: the (1) Sham, (2) Ovx, (3) Ovx+Exe, (4) Ovx+MR, (5) Ovx+MR+Exe and (6) Ovx+Est groups. Rats in the exercise group were subjected to a 12-week treadmill running training regime (16 m/min, 60 min/day and 5 days/week) started from two weeks before sham or Ovx surgery. Methionine restriction diet was begun 1 week after Ovx surgery. All animals were free access to feed, and body weight and food intake were measured twice per week. After the end of 12-week intervention, all animals were sacrificed under deep anesthesia. Samples of serum and bone were collected and prepared for analyses of serum marker assays, static histomorphometry, dynamic histomorphometry and biomechanical properties. One-way ANOVA or one-way ANCOVA was used for data processing in the current study. Fisher’s least significant difference (LSD) method was used for post hoc comparison when p< .05.
Results: After 12-week experiment period, Exe and MR diet, respectively, showed different efficacies in mitigating ovx-induced body weight gain. In serum bone markers, the Sham and the Ovx+Est groups revealed significantly lower osteocalcin and C-telopeptide fragments of collagen type 1 (CTX-1) levels as compared to the other groups and the Ovx group, respectively. In static histomorphometry, the Ovx+Exe group showed significantly lower bone volume (BV)and bone volume ratio (BV/TV) than the Sham and Ovx+Est groups whereas significantly higher as compared to the Ovx, Ovx+MR and Ovx+MR+Exe groups. In trabecular thickness, two MR groups were significantly lower as compared to the Sham, Ovx+Exe and Ovx+Est groups. The Ovx+Exe group showed significantly higher Tb.N and Conn.Dn. as compared to the Ovx and two MR groups. In densitometry, the Ovx group was significantly lowerer in whole femoral bone mineral content (BMC) value than the other groups. The Ovx+Exe and Ovx+MR groups were significantly higher in whole femoral bone mineral density (BMD) than the Sham and Ovx+Est groups. In dynamic histomorphometry, the Ovx, Ovx+Exe and Ovx+MR groups were significantly higher in mineral surface/bone surface (MS/BS) of secondary spongiosa as compared to the other groups. The Ovx group showed significantly higher MS/BS in endocortical bone surface than all other groups except for the Ovx+Exe group. And, the Ovx+Exe group was significantly higher in periosteal MS/BS than the other groups except for its non-difference to the Ovx group. In biomechanical properties, the Ovx group was significantly lower than the other groups in yield load, max load and fracture load.
Conclusion: MR diet and Exe showed difference efficacies in protecting ovariectomy caused spongy and/or cortical bone loss. And, endurance exercise revealed a more significant efficiency in defending bone from ovx-induced osteopenia. Since MR diet is one of the diet models, which simulates vegan diet in vivo experiment, further studies would be worthy to investigate the effects of MR diet on bone metabolism as well as bone qualities through clinical trials in normal/menopause subjects.
論文目次 摘 要 I
Abstract III
致 謝 V
Table of Contents VI
List of Tables VII
List of Figures VIII
Abbreviations IX
Introduction 1
Operational Definition 4
Materials and Methods 5
Animals 5
Experimental design 5
MR diet 5
Exercise training protocol 7
Ovariectomy surgery 7
Animal sacrifice 7
Blood samples 8
Bone sample collection and measurements 8
Static histomorphometry 9
Dynamic histomorphometry 10
Biomechanical three-point bending testing 11
Statistical analysis 12
Results 13
Discussion 31
Conclusion 33
References 34
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