進階搜尋


下載電子全文  
系統識別號 U0026-2306201113324100
論文名稱(中文) 口腔癌細胞特定位點氮基醣鏈之分析
論文名稱(英文) Site-specific N-glycan Analysis of Oral Cancer Cell
校院名稱 成功大學
系所名稱(中) 醫學檢驗生物技術學系碩博士班
系所名稱(英) Department of Medical Laboratory Science and Biotechnology
學年度 99
學期 2
出版年 100
研究生(中文) 張逸敏
研究生(英文) Yi-Min Chong
學號 T36984051
學位類別 碩士
語文別 英文
論文頁數 90頁
口試委員 指導教授-張權發
口試委員-謝達斌
口試委員-廖寶琦
口試委員-羅順原
中文關鍵字 氮基醣鏈  核心岩藻醣化  唾液酸醣化  口腔癌 
英文關鍵字 N-glycan  Core-fucosylation  Sialylation  Oral Cancer 
學科別分類
中文摘要 在真核細胞內超過半數的蛋白質會被醣化,而這些醣類結構的改變在癌症的病程發展中扮演了重要的角色。在實驗中,我們分析了三種不同狀態的細胞上的氮基醣鏈,包括正常人類初代口腔細胞 (HOK)、正常口腔齒齦細胞株 (SG) 和人類口腔癌細胞株(OC2)。利用化學標定的方式,我們偵測在這三株細胞內的兩群組酵素的活性:岩藻醣轉移酶 (fucosyltransferases, FUT)和唾液酸轉移酶 (sialyltransferases, ST)。接著我們進一步利用即時定量聚合酶連鎖反應來偵測各個岩藻醣轉移酶和唾液酸轉移酶的mRNA的表現量。相較於HOK和SG這兩個正常細胞株,OC2具有較高的FUT8表現量,其整體唾液酸轉移酶(ST)的表現量也是較高的。接著我們利用質譜的方式去分析細胞上方的氮基醣鏈,發現OC2上含有較豐富的核心岩藻醣化 (core-fucosylated) 以及唾液酸醣化 (sialylated) 的氮基醣鏈 (complex type),然而HOK和SG上是以高甘露醣鏈 (high mannose) 類型的氮基醣鏈為主。從上述實驗結果,我們推論蛋白質上的唾液酸醣化以及核心岩藻醣化對於口腔癌的病程發展可能具有一定的意義。進一步利用凝集素親和性層析法純化和質譜儀,我們純化並鑑定了OC2當中有核心岩藻醣化的蛋白質。其中,參與在密切參與在細胞間交互作用和細胞移行的蛋白質分子例如basigin、ALCAM、4F2和integrins可做為未來探討口腔癌生物標誌的參考。
英文摘要 More than half proteins in eukaryotes are glycosylated. Alteration of glycosylation in glycoproteins is known to involve in mediating key pathophysiological events during tumorigenesis. In this study, we aimed to establish a MS based strategy for glycomic mapping and sequencing for cell lines and investigated the N-glycosylation status of OC2 (oral cancer cells), SG cells (Smulow-Glikcman gingival cells) which were immortalized from normal human gingival and HOK (primary human oral keratinocytes). The semiquantitative mRNA results indicated that OC2 have higher FUT8 and overall sialyltransferases mRNA levels compared to HOK and SG cells. In addition, we analyzed the cell surface N-glycan profiles and monosaccharide composition of OC2, SG and HOK cells by MALDI-mass spectrometry and tandem mass spectrometry. We found that the carbohydrate patterns of OC2 were different from SG and HOK cells. The results also indicated that the sialylated and core-fucosylated N-glycans were highly expressed on the cell surface of OC2, whereas the major N-glycans on the SG cells and HOK were high mannose types. Combine these information, we suggested that the α1-6 core fucosylation and terminal sialylation were significant issues during tumorigenesis. Using AAL lectin-affinity chromatography combined with MS, we identified the core-fucosylated proteins that derived from OC2. The key adhesion molecules such as basigin, ALCAM, 4F2 and integrins could be considered as the candidates of potential oral cancer biomarkers in the future studies.
論文目次 摘要 I
Abstract II
Acknowledgement III
Abbreviations IV
Contents VI
List of Figures IX
List of Tables X
Chapter 1 Introduction 1
1-1 Glycosylation 1
1-2 Alteration of Glycosylation in Cancer 4
1-3 Oral Cancer in Taiwan 5
1-4 Introduction of Glycomics & Glycoproteomics 6
1-4.1 Definition of Glycomics & Glycoproteomics 6
1-4.2 Glycoproteomics & Glycomics strategies 6
Chapter 2 Study Objective and Specific Aims 9
2-1 Study Objective 9
2-2 Specific Aims 9
2-3 Study Flow Chart 10
Chapter 3 Experimental Procedures 11
3-1 Cell culture 11
3-2 RNA isolation and real-time quantitative PCR analysis 11
3-3 Metabolic labeling for fluorescent imaging of fucosylated and sialylated glycans 12
3-4 MALDI-MS analysis of permethylated N-glycans 12
3-4.1 Cell membrane preparation and membrane protein extraction 12
3-4.2 Trypsin digestion of proteins 13
3-4.3 Release and purification of N-glycans 13
3-4.4 Permethylation of N-glycans 14
3-4.5 Mass analysis and data evaluation 14
3-5 Lectin affinity chromatography and protein identification 14
3-5.1 AAL – Affinity chromatography 14
3-5.2 TCA protein precipitation 15
3-5.3 SDS-PAGE and gel staining 15
3-5.4 In-gel digestion 16
3-5.5 MS analysis and proteins identification 16
Chapter 4 Results 17
4-1 Metabolic labeling of fucosylated and sialylated glycoproteins in HOK, SG and OC2 cells. 17
4-2 Expression levels of fucosyltransferase genes in HOK, SG and OC2 cells. 18
4-3 Expression levels of sialyltransferase genes in HOK, SG and OC2 cells. 18
4-4 N-glycome analysis in HOK, SG and OC2 cells. 19
4-4.1 MS analysis of N-glycan pools 19
4-4.2 The MS/MS analysis of N-glycan pools 21
4-5 Enrichment and identification of core-fucosylated glycoproteins in OC2 cells. 23
Chapter 5 Discussions 24
5-1 Alteration of fucosylated and sialylated glycoconjugates in cancer 24
5-2 The cell models in this work 25
5-3 The transcript levels of fucosyltransferase and sialyltransferase family in HOK, SG and OC2 cells. 25
5-4 The biological roles of FUT8 in OC2 cells. 26
5-5 The N-glycome analysis in HOK, SG and OC2 cells. 27
5-6 Enrichment and identification of core-fucosylated glycoproteins in OC2 cells. 28
Conclusion 32
References 33
Figures and Figure legends 42
Table 58
Appendix -1 84
Appendix -2 85
Appendix -3 86
Appendix -4 87
參考文獻 Abd Hamid, U. M., L. Royle, et al. (2008). "A strategy to reveal potential glycan markers from serum glycoproteins associated with breast cancer progression." Glycobiology 18(12): 1105-1118.
Aoyagi, Y. (1995). "Carbohydrate-based measurements on alpha-fetoprotein in the early diagnosis of hepatocellular carcinoma." Glycoconj J 12(3): 194-199.
Apweiler, R., H. Hermjakob, et al. (1999). "On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database." Biochimica Et Biophysica Acta-General Subjects 1473(1): 4-8.
Arcari, P., R. Martinelli, et al. (1984). "The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species." Nucleic Acids Res 12(23): 9179-9189.
Arnold, J. N., M. R. Wormald, et al. (2007). "The impact of glycosylation on the biological function and structure of human immunoglobulins." Annual Review of Immunology 25: 21-50.
Axford, J. (1997). "Glycobiology and medicine: an introduction." J R Soc Med 90(5): 260-264.
Babich, H. and D. A. Tipton (1999). "In vitro cytotoxicity of bisphenol A to human gingival epithelial S-G cells." In Vitro & Molecular Toxicology-a Journal of Basic and Applied Research 12(4): 233-244.
Becker, D. J. and J. B. Lowe (2003). "Fucose: biosynthesis and biological function in mammals." Glycobiology 13(7): 41R-53R.
Bond, M. R. and J. J. Kohler (2007). "Chemical methods for glycoprotein discovery." Current Opinion in Chemical Biology 11(1): 52-58.
Bordador, L. C., X. W. Li, et al. (2000). "Expression of EMMPRIN by oral squamous cell carcinoma." International Journal of Cancer 85(3): 347-352.
Bryne, M., P. S. Thrane, et al. (1990). "Loss of Expression of Blood-Group Antigen-H Is Associated with Cellular Invasion and Spread of Oral Squamous-Cell Carcinomas." Cancer 66(10): 2118-2123.
Bumol, T. F., D. O. Chee, et al. (1982). "Immunochemical and biosynthetic analysis of monoclonal antibody-defined melanoma-associated antigen." Hybridoma 1(3): 283-292.


Carlsson, S. R. and M. Fukuda (1990). "The Polylactosaminoglycans of Human Lysosomal Membrane-Glycoproteins Lamp-1 and Lamp-2 - Localization on the Peptide Backbones." Journal of Biological Chemistry 265(33): 20488-20495.
Chang, H. (2005). "Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA." Biomaterials 26(7): 745-753.
Chen, R., X. N. Jiang, et al. (2009). "Glycoproteomics Analysis of Human Liver Tissue by Combination of Multiple Enzyme Digestion and Hydrazide Chemistry." Journal of Proteome Research 8(2): 651-661.
Dall'Olio, F. and M. Chiricolo (2001). "Sialyltransferases in cancer." Glycoconjugate Journal 18(11-12): 841-850.
Dalpathado, D. S. and H. Desaire (2008). "Glycopeptide analysis by mass spectrometry." Analyst 133(6): 731-738.
Dang, D. M., A. Atakilit, et al. (2008). "EMMPRIN modulates migration and deposition of TN-C in oral squamous carcinoma." Anticancer Research 28(4B): 2049-2054.
de Leoz, M. L. A., L. J. T. Young, et al. (2010). "High-Mannose Glycans are Elevated during Breast Cancer Progression." Molecular & Cellular Proteomics 10(1): M110.002717-M002110.002717.
Dell, A. (2001). "Glycoprotein Structure Determination by Mass Spectrometry." Science 291(5512): 2351-2356.
Dennis, J. W. and S. Laferte (1989). "Oncodevelopmental Expression of -Glcnac-Beta-1-6man-Alpha-1-6man-Beta-1-Branched Asparagine-Linked Oligosaccharides in Murine Tissues and Human-Breast Carcinomas." Cancer Research 49(4): 945-950.
Dennis, J. W., S. Laferte, et al. (1987). "Beta-1-6 Branching of Asn-Linked Oligosaccharides Is Directly Associated with Metastasis." Science 236(4801): 582-585.
Driouich, A., P. Gonnet, et al. (1989). "The Role of High-Mannose and Complex Asparagine-Linked Glycans in the Secretion and Stability of Glycoproteins." Planta 180(1): 96-104.
Esseghir, S., J. S. Reis, et al. (2006). "Identification of transmembrane proteins as potential prognostic markers and therapeutic targets in breast cancer by a screen for signal sequence encoding transcripts." Journal of Pathology 210(4): 420-430.
Esteban, F., F. Ruizcabello, et al. (1990). "Relationship of 4f2-Antigen with Local Growth and Metastatic Potential of Squamous-Cell Carcinoma of the Larynx." Cancer 66(7): 1493-1498.

Fenczik, C. A., T. Sethi, et al. (1997). "Complementation of dominant suppression implicates CD98 in integrin activation." Nature 390(6655): 81-85.
G.N. Raval, L. J. P., D.D. Patel, F.P. Jha, R.N. Sainger and P.S. Patel (2004). "Clinical Usefulness of Alterations in Sialic Acid, Sialyltransferase and Sialoproteins in Breast Cancer." Indian Journal of Clinical Biochemistry 19(2): 60-71.
Geyer, H. and R. Geyer (2006). "Strategies for analysis of glycoprotein glycosylation." Biochimica Et Biophysica Acta-Proteins and Proteomics 1764(12): 1853-1869.
Giancotti, F. G. and E. Ruoslahti (1999). "Transduction - Integrin signaling." Science 285(5430): 1028-1032.
Goelz, S. E., C. Hession, et al. (1990). "ELFT: a gene that directs the expression of an ELAM-1 ligand." Cell 63(6): 1349-1356.
Goetz, J. A., Y. Mechref, et al. (2009). "Glycomic profiling of invasive and non-invasive breast cancer cells." Glycoconj J 26(2): 117-131.
Grundmann, U., C. Nerlich, et al. (1990). "Complete cDNA sequence encoding human beta-galactoside alpha-2,6-sialyltransferase." Nucleic Acids Res 18(3): 667.
Harduin-Lepers, A., V. Vallejo-Ruiz, et al. (2001). "The human sialyltransferase family." Biochimie 83(8): 727-737.
Helenius, A. and M. Aebi (2004). "Roles of N-linked glycans in the endoplasmic reticulum." Annu Rev Biochem 73: 1019-1049.
Hiroyasu, D. S. K. H. K. H. T. K. O. Y. T. M. I. S. I. T. M. (2003). "Sialyl Lex and Sialyl Lea expression in human extrahepatic bile duct adenocarcinoma." Biomedical research 24(6): 277-283.
Holmes, C. H., B. Gunn, et al. (1982). "Expression of a monoclonal antibody-defined liver-associated antigen in normal rat hepatocytes and hepatocellular carcinoma cells." Int J Cancer 29(5): 559-565.
Hsu, T. L., S. R. Hanson, et al. (2007). "Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells." Proceedings of the National Academy of Sciences of the United States of America 104(8): 2614-2619.
Hynes, R. O. (2002). "Integrins: Bidirectional, allosteric signaling machines." Cell 110(6): 673-687.
Ikehara, Y., N. Kojima, et al. (1999). "Cloning and expression of a human gene encoding an N-acetylgalactosamine-alpha 2,6-sialyltransferase (ST6GalNAc I): a candidate for synthesis of cancer-associated sialyl-Tn antigens." Glycobiology 9(11): 1213-1224.
Imai-Nishiya, H., K. Mori, et al. (2007). "Double knockdown of alpha1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC." BMC Biotechnology 7(1): 84.
Imperiali, B. and S. E. O'Connor (1999). "Effect of N-linked glycosylation on glycopeptide and glycoprotein structure." Curr Opin Chem Biol 3(6): 643-649.
Ishii, A., M. Ohta, et al. (1998). "Expression cloning and functional characterization of human cDNA for ganglioside GM3 synthase." Journal of Biological Chemistry 273(48): 31652-31655.
Ito, Y., A. Miyauchi, et al. (2003). "Expression of alpha1,6-fucosyltransferase (FUT8) in papillary carcinoma of the thyroid: its linkage to biological aggressiveness and anaplastic transformation." Cancer Lett 200(2): 167-172.
Kaira, K., N. Oriuchi, et al. (2008). "l-type amino acid transporter 1 and CD98 expression in primary and metastatic sites of human neoplasms." Cancer Science 99(12): 2380-2386.
Kanekura, T., X. Chen, et al. (2002). "Basigin (CD147) is expressed on melanoma cells and induces tumor cell invasion by stimulating production of matrix metalloproteinases by fibroblasts." International Journal of Cancer 99(4): 520-528.
Kao, S. Y., Y. W. Chu, et al. (2009). "Detection and Screening of Oral Cancer and Pre-cancerous Lesions." Journal of the Chinese Medical Association 72(5): 227-233.
Kasten, F. H., L. F. R. Pineda, et al. (1989). "Biocompatibility Testing of an Experimental Fluoride Releasing Resin Using Human Gingival Epithelial-Cells Invitro." In Vitro Cellular & Developmental Biology 25(1): 57-62.
Kelly, R. J., S. Rouquier, et al. (1995). "Sequence and expression of a candidate for the human Secretor blood group alpha(1,2)fucosyltransferase gene (FUT2). Homozygosity for an enzyme-inactivating nonsense mutation commonly correlates with the non-secretor phenotype." J Biol Chem 270(9): 4640-4649.
Kim, Y. J., K. S. Kim, et al. (1996). "Molecular cloning and expression of human Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (hST3Gal II)." Biochemical and Biophysical Research Communications 228(2): 324-327.
King, J. A., S. F. Ofori-Acquah, et al. (2004). "Activated leukocyte cell adhesion molecule in breast cancer: prognostic indicator." Breast Cancer Research 6(5): R478-R487.
Kitagawa, H. and J. C. Paulson (1993). "Cloning and Expression of Human Gal-Beta-1,3(4)Glcnac Alpha-2,3-Sialyltransferase." Biochemical and Biophysical Research Communications 194(1): 375-382.
Ko, Y. C., Y. L. Huang, et al. (1995). "Betel quid chewing, cigarette smoking and alcohol consumption related to oral cancer in Taiwan." J Oral Pathol Med 24(10): 450-453.
Kornfeld, R. and S. Kornfeld (1985). "Assembly of Asparagine-Linked Oligosaccharides." Annual Review of Biochemistry 54: 631-664.
Krolikowski, F. J., K. Reuter, et al. (1976). "Serum sialic acid levels in lung cancer patients." Pharmacology 14(1): 47-51.
Kyselova, Z., Y. Mechref, et al. (2007). "Alterations in the serum glycome due to metastatic prostate cancer." Journal of Proteome Research 6(5): 1822-1832.
Larsen, R. D., L. K. Ernst, et al. (1990). "Molecular cloning, sequence, and expression of a human GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase cDNA that can form the H blood group antigen." Proc Natl Acad Sci U S A 87(17): 6674-6678.
Lee, Y. C. and R. T. Lee (1995). "Carbohydrate-Protein Interactions - Basis of Glycobiology." Accounts of Chemical Research 28(8): 321-327.
Lewandrowski, U., J. Moebius, et al. (2006). "Elucidation of N-glycosylation sites on human platelet proteins - A glycoproteomic approach." Molecular & Cellular Proteomics 5(2): 226-233.
Li, L. B., N. H. Chen, et al. (2004). "The role of N-glycosylation in function and surface trafficking of the human dopamine transporter." Journal of Biological Chemistry 279(20): 21012-21020.
Li, W. (2006). "Down-regulation of trypsinogen expression is associated with growth retardation in 1,6-fucosyltransferase-deficient mice: attenuation of proteinase-activated receptor 2 activity." Glycobiology 16(10): 1007-1019.
Liang, S. M., C. P. Huang, et al. (2011). "Activated Leukocyte Cell Adhesion Molecule Expression Is Up-Regulated in the Development of Endometrioid Carcinoma." International Journal of Gynecological Cancer 21(3): 523-528.
Liu, T., W. J. Qian, et al. (2005). "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Journal of Proteome Research 4(6): 2070-2080.
Lopez-Morales, D., J. Reyes-Leyva, et al. (2010). "Increased expression of sialic acid in cervical biopsies with squamous intraepithelial lesions." Diagn Pathol 5: 74.
Lu, X. Y., A. Mehta, et al. (1995). "Evidence That N-Linked Glycosylation Is Necessary for Hepatitis-B Virus Secretion." Virology 213(2): 660-665.
Manoharan, S., M. Padmanabhan, et al. (2004). "Analysis of glycoconjugates in patients with oral squamous cell carcinoma." Clinica Chimica Acta 339(1-2): 91-96.
Merlin, D., S. Sitaraman, et al. (2001). "CD98-mediated links between amino acid transport and beta(1) integrin distribution in polarized columnar epithelia." Journal of Biological Chemistry 276(42): 39282-39289.
Miyoshi, E., K. Noda, et al. (1999). "The alpha1-6-fucosyltransferase gene and its biological significance." Biochim Biophys Acta 1473(1): 9-20.




Montiel, M. D., M. A. Krzewinski-Recchi, et al. (2003). "Molecular cloning, gene organization and expression of the human UDP-GalNAc : Neu5Ac alpha 2-3Gal beta-R beta l,4-N-acetylgalactosaminyltransferase responsible for the biosynthesis of the blood group Sd(a)/Cad antigen: evidence for an unusual extended cytoplasmic domain." Biochemical Journal 373: 369-379.
Morelle, W. and J. C. Michalski (2007). "Analysis of protein glycosylation by mass spectrometry." Nat Protoc 2(7): 1585-1602.
Morishima, S., I. Morita, et al. (2003). "Expression and role of mannose receptor/terminal high-mannose type oligosaccharide on osteoclast precursors during osteoclast formation." J Endocrinol 176(2): 285-292.
Muinelo-Romay, L., C. Vazquez-Martin, et al. (2008). "Expression and enzyme activity of alpha(1,6)fucosyltransferase in human colorectal cancer." International Journal of Cancer 123(3): 641-646.
Muraoka, K., K. Nabeshima, et al. (1993). "Enhanced Expression of a Tumor-Cell-Derived Collagenase-Stimulatory Factor in Urothelial Carcinoma - Its Usefulness as a Tumor-Marker for Bladder Cancers." International Journal of Cancer 55(1): 19-26.
Noda, K., E. Miyoshi, et al. (1998). "Gene expression of alpha 1-6 fucosyltransferase in human hepatoma tissues: A possible implication for increased fucosylation of alpha-fetoprotein." Hepatology 28(4): 944-952.
North, S. J., P. G. Hitchen, et al. (2009). "Mass spectrometry in the analysis of N-linked and O-linked glycans." Curr Opin Struct Biol 19(5): 498-506.
Norton, P. A., M. A. Comunale, et al. (2008). "N-linked glycosylation of the liver cancer biomarker GP73." J Cell Biochem 104(1): 136-149.
Ofori-Acquah, S. F. and J. A. King (2008). "Activated leukocyte cell adhesion molecule: a new paradox in cancer." Transl Res 151(3): 122-128.
Osumi, D., M. Takahashi, et al. (2009). "Core fucosylation of E-cadherin enhances cell-cell adhesion in human colon carcinoma WiDr cells." Cancer Science 100(5): 888-895.
Polette, M., C. Gilles, et al. (1997). "Tumor collagenase stimulatory factor (TCSF) expression and localization in human lung and breast cancers." Journal of Histochemistry & Cytochemistry 45(5): 703-709.
Prager, G. W., M. Poettler, et al. (2009). "CD98hc (SLC3A2), a novel marker in renal cell cancer." European Journal of Clinical Investigation 39(4): 304-310.
Rajpura, K. B., P. S. Patel, et al. (2005). "Clinical significance of total and lipid bound sialic acid levels in oral pre-cancerous conditions and oral cancer." J Oral Pathol Med 34(5): 263-267.
Roseman, S. (2001). "Reflections on glycobiology." Journal of Biological Chemistry 276(45): 41527-41542.
Samyn-Petit, B., M. A. Krzewinski-Recchi, et al. (2000). "Molecular cloning and functional expression of human ST6GalNAc II. Molecular expression in various human cultured cells." Biochimica Et Biophysica Acta-General Subjects 1474(2): 201-211.
Sasaki, K., K. Kurata, et al. (1994). "Expression cloning of a novel alpha 1,3-fucosyltransferase that is involved in biosynthesis of the sialyl Lewis x carbohydrate determinants in leukocytes." J Biol Chem 269(20): 14730-14737.
Sasaki, K., E. Watanabe, et al. (1993). "Expression Cloning of a Novel Gal-Beta(1-3/1-4)G1cnac Alpha-2,3-Sialyltransferase Using Lectin Resistance Selection." Journal of Biological Chemistry 268(30): 22782-22787.
Saussez, S., H. Marchant, et al. (1998). "Quantitative glycohistochemistry defines new prognostic markers for cancers of the oral cavity." Cancer 82(2): 252-260.
Sawa, M., T. L. Hsu, et al. (2006). "Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo." Proc Natl Acad Sci U S A 103(33): 12371-12376.
Sekine, C., Y. Aoyagi, et al. (1987). "The reactivity of alpha-1-antitrypsin with Lens culinaris agglutinin and its usefulness in the diagnosis of neoplastic diseases of the liver." Br J Cancer 56(3): 371-375.
Shah, M., S. Telang, et al. (2008). "Serum fucosylation changes in oral cancer and oral precancerous conditions." Cancer 113(2): 336-346.
Shang, J., R. Qiu, et al. (1999). "Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase from human fetal liver." Eur J Biochem 265(2): 580-588.
Shida, K., Y. Misonou, et al. (2009). "Unusual accumulation of sulfated glycosphingolipids in colon cancer cells." Glycobiology 19(9): 1018-1033.
Shpitzer, T., Y. Hamzany, et al. (2009). "Salivary analysis of oral cancer biomarkers." British Journal of Cancer 101(7): 1194-1198.
Sjoberg, E. R., H. Kitagawa, et al. (1996). "Molecular cloning of a developmentally regulated N-acetylgalactosamine alpha 2,6-sialyltransferase specific for sialylated glycoconjugates." Journal of Biological Chemistry 271(13): 7450-7459.
Smulow, J. B. and I. Glickman (1966). "An epithelial-like cell line in continuous culture from normal adult human gingiva." Proc Soc Exp Biol Med 121(4): 1294-1296.
Sozzani, P., R. Arisio, et al. (2008). "Is sialyl Lewis x antigen expression a prognostic factor in patients with breast cancer?" International Journal of Surgical Pathology 16(4): 365-374.
Spiro, R. G. (2002). "Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds." Glycobiology 12(4): 43r-56r.
Stanley, P. (1992). "Glycosylation engineering." Glycobiology 2(2): 99-107.

Strohalm, M., D. Kavan, et al. (2010). "mMass 3: A Cross-Platform Software Environment for Precise Analysis of Mass Spectrometric Data." Analytical Chemistry 82(11): 4648-4651.
Su, J., X. Chen, et al. (2009). "A CD147-targeting siRNA inhibits the proliferation, invasiveness and VEGF production of human malignant melanoma cells by down-regulating glycolysis." Cancer Letters 273(1): 140-147.
Suzuki, Y., T. Ito, et al. (2000). "Sialic acid species as a determinant of the host range of influenza A viruses." Journal of Virology 74(24): 11825-11831.
Takahashi, M., Y. Kuroki, et al. (2009). "Core fucose and bisecting GlcNAc, the direct modifiers of the N-glycan core: their functions and target proteins." Carbohydrate Research 344(12): 1387-1390.
Takahashi, T., Y. Ueda, et al. (2000). "alpha 1,6Fucosyltransferase is highly and specifically expressed in human ovarian serous adenocarcinomas." International Journal of Cancer 88(6): 914-919.
Takano, R., M. Nose, et al. (1990). "Increase of beta 1-6-branched oligosaccharides in human esophageal carcinomas invasive against surrounding tissue in vivo and in vitro." Am J Pathol 137(5): 1007-1011.
Turner, G. A. (1992). "N-glycosylation of serum proteins in disease and its investigation using lectins." Clin Chim Acta 208(3): 149-171.
Varki, A. (2009). Essentials of glycobiology. Cold Spring Harbor, N.Y., Cold Spring Harbor Laboratory Press.
Varki, A., R. D. Cummings, et al. (2009). "Symbol nomenclature for glycan representation." Proteomics 9(24): 5398-5399.
Verma, A., N. K. Shukla, et al. (2005). "MEMD/ALCAM: A potential marker for tumor invasion and nodal metastasis in esophageal squamous cell carcinoma." Oncology 68(4-6): 462-470.
Vigneswaran, N., S. Beckers, et al. (2006). "Increased EMMPRIN (CD 147) expression during oral carcinogenesis." Experimental and Molecular Pathology 80(2): 147-159.
Weichert, W., T. Knosel, et al. (2004). "ALCAM/CD166 is overexpressed in colorectal carcinoma and correlates with shortened patient survival." Journal of Clinical Pathology 57(11): 1160-1164.
West, I. and O. Goldring (1994). Lectin affinity chromatography. Mol Biotechnol. 2: 147-155.
Weston, B. W., P. L. Smith, et al. (1992). "Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes." J Biol Chem 267(34): 24575-24584.
Wollscheid, B., D. Bausch-Fluck, et al. (2009). "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins (vol 27, pg 378, 2009)." Nature Biotechnology 27(9): 864-864.
Wong, D. Y., K. W. Chang, et al. (1990). "Characterization of two new cell lines derived from oral cavity human squamous cell carcinomas--OC1 and OC2." J Oral Maxillofac Surg 48(4): 385-390.
Wu, J. Y., C. Yi, et al. (2010). "Potential biomarkers in saliva for oral squamous cell carcinoma." Oral Oncology 46(4): 226-231.
Yamashita, F., M. Tanaka, et al. (1996). "Prognostic significance of Lens culinaris agglutinin A-reactive alpha-fetoprotein in small hepatocellular carcinomas." Gastroenterology 111(4): 996-1001.
Yamashita, K., N. Kochibe, et al. (1985). "Fractionation of L-Fucose-Containing Oligosaccharides on Immobilized Aleuria-Aurantia Lectin." Journal of Biological Chemistry 260(8): 4688-4693.
Yanagidani, S., N. Uozumi, et al. (1997). "Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells." J Biochem 121(3): 626-632.
Zaia, J. (2010). "Mass Spectrometry and Glycomics." Omics-a Journal of Integrative Biology 14(4): 401-418.
Zhang, H., X. J. Li, et al. (2003). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Nature Biotechnology 21(6): 660-666.
Zhang, S., C. Cordon-Cardo, et al. (1997). "Selection of tumor antigens as targets for immune attack using immunohistochemistry: I. Focus on gangliosides." Int J Cancer 73(1): 42-49.
Zhang, S., H. S. Zhang, et al. (1998). "Selection of tumor antigens as targets for immune attack using immunohistochemistry: protein antigens." Clin Cancer Res 4(11): 2669-2676.
Zhao, Y., Y. Sato, et al. (2008). "Branched N-glycans regulate the biological functions of integrins and cadherins." Febs Journal 275(9): 1939-1948.

論文全文使用權限
  • 同意授權校內瀏覽/列印電子全文服務,於2012-07-18起公開。
  • 同意授權校外瀏覽/列印電子全文服務,於2013-07-18起公開。


  • 如您有疑問,請聯絡圖書館
    聯絡電話:(06)2757575#65773
    聯絡E-mail:etds@email.ncku.edu.tw