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系統識別號 U0026-2208201021461600
論文名稱(中文) 探討在自發性失神癲癇大鼠長期給予lamotrigine對癲癇與似焦慮/憂鬱行為之影響
論文名稱(英文) Chronic lamotrigine treatment in seizure and anxiety-/depression-like behaviors of the rat with spontaneous absence epilepsy
校院名稱 成功大學
系所名稱(中) 認知科學研究所
系所名稱(英) Institute of Cognitive Science
學年度 98
學期 2
出版年 99
研究生(中文) 李曉薇
研究生(英文) Hsiao-Wei Lee
學號 u7697404
學位類別 碩士
語文別 英文
論文頁數 40頁
口試委員 指導教授-蕭富仁
口試委員-嚴震東
口試委員-梁勝富
中文關鍵字 Lamotrigine  Long-Evans大鼠  棘徐波  焦慮行為  憂鬱行為  開放空間  架高十字迷宮  糖水測試  強迫游泳測試 
英文關鍵字 Lamotrigine  Long-Evans rats  spontaneous spike-wave discharges  anxiety  depression  open field  elevated plus maze  sucrose consumption  forced swimming 
學科別分類
中文摘要 Lamotrigine (LTG)是一種新型的抗癲癇藥物,在臨床上常用來治療失神性癲癇。然而,目前LTG在癲癇動物實驗結果卻出現與臨床失神癲癇病患不一致的現象,結果的主要差異可能是給LTG的方式不同(單次[動物實驗]vs長期[臨床使用])所造成。為了進一步釐清LTG在失神癲癇動物實驗的效果以及了解LTG對於癲癇併發產生焦慮與憂鬱的影響,本論文比較會自發性產生棘徐波(spike-wave discharge, SWD)的Long-Evans大鼠在長期給予LTG或飲用水之後發現:(1)、單次給予LTG治療無法抑制SWD;(2)、經過35天的LTG治療,能夠有效抑制SWD發作的總時間、SWD次數、以及SWD平均時間,而控制組大鼠則沒有統計上的差異;長期給予LTG大鼠會比控制組大鼠出現較長時間穿越開放空間(open field test)的中央區域以及較長時間探索架高型十字迷宮(elevated plus maze test)的開放支架等現象,其結果都達到統計上顯著的差異;(4)、在強迫游泳測試(forced swimming test)中,長期給予LTG大鼠的不動時間顯著短於控制組大鼠的不動時間。本論文證實LTG在以臨床類似狀況下長期給予之後可以顯著地抑制失神癲癇,並且同時改善失神癲癇引發之似焦慮與似憂鬱行為。
英文摘要 Lamotrigine (LTG), an antiepileptic drug, is a prescription for both convulsive and absence seizures in clinic. However, LTG effects are quite controversial between epileptic animal experiments and patients. Different administering route and duration may be reasons to produce the discrepancy. To understand LTG effect on absence seizures and on its comorbidity of anxiety and depression, LTG (10 mg/kg) or water was chronically peroral administered twice daily on Long-Evans rats with spontaneous spike-wave discharges (SWDs) for 35 days. Our results showed that acute administration of LTG had no benefit in suppression of absence seizures. Total SWD duration, SWD number, and mean SWD duration were significantly decreased in rats received 35-day LTG compared to those of rats with vehicle. Moreover, rats having LTG treatment displayed significantly longer duration entering the central zone of an open field and longer duration exploring open arm of an elevated plus maze compared to those of the control group. Immobile duration during a forced swimming test was significantly shorter in rats with LTG than those of rats with vehicle. Our results suggest that chronic LTG treatment has a great benefit on suppression of absence seizures and also ameliorates anxiety and depression.
論文目次 中文摘要 I
Abstract II
Acknowledgment IV
Content VI
List of Figures VIII
Abbreviation IX
Introduction 1
Absence epilepsy 1
Depression/anxiety 2
Long-Evans rats with spontaneous spike wave discharges 3
Lamotrigine 4
Methods 6
Animal preparations and recordings 6
Drug Preparation 7
Behavioral tests 8
Procedural of experiment 11
Statistical analysis 12
Results 13
Electroencephalogram recordings 13
Open field test 15
Elevated plus maze test 15
Sucrose consumption test 16
Forced swimming test 16
Discussion 18
Major findings 18
Lamotrigine treatment 18
Anxiety-like behaviors under chronic lamotrigine 19
Depression-like behaviors under chronic lamotrigine 20
Conclusion 23
Future work 24
References 25
Figures 33
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