||Social defeat /psychosocial stress pre-exposure produces increases in the acetic acid-induced writhing possibly via upregulation of TRPV1 receptor in the nucleus accumbens
||Department of Physiology
Exposure to repeated stressor is associated with the risk of our health. It has been documented that psychosocial stress may devastate the self-reported physical pain. Nevertheless, sparse reports ever address the impact of the psychosocial stress on modulating the magnitude of visceral pain. Visceral pain is used to describe pain initiating from the internal organs in viscera, which is distinct from somatic pain. This study was undertaken to assess psychosocial stress pre-exposure may modulate the magnitudes of visceral pain and group housing may diminish such the effect of psychosocial stress. Six consecutive days of social defeat mouse model were used to mimic psychosocial stress. A single intraperitoneal acetic acid injection was used to model acute visceral pain and the writhing responses were to indicate such visceral pain magnitudes. Group housing referred to observing the acid-induced writhing responses in a group of 3 mice. We found that 6-day psychosocial stress pre-exposure indeed facilitated the number of acid-induced writhing responses as compared to the mice receiving no such stress pre-exposure. Moreover, the presence of two other acid-treated conspecifics mice in this mouse model demonstrated effectively attenuate the number of writhing responses. Systemic administration (0.25 mg/kg) of SB366791, a transient receptor potential vanilloid receptor type 1 ( TRPV1 ) antagonist, prevented increment of acid-induced writhing responses following psychosocial stress exposure, although the SB366791 treatment did not affect the writhing in stress-free mice. In contrast, the SB366791 administration did not affect the group housing exerts effects against the psychosocial stress-primed increases in acid-induced writhing. Furthermore, the expression of TRPV1 receptors had increased in the nucleus accumbens on the psychosocial stress pre-exposure mice. Finally, we found that neuronal activity was lower in the medial septal nucleus in the presence of two other acid-treated conspecifics mice. Per the study, results showed that psychosocial stress pre-exposure may enhance the acid-induced writhing and increase TRPV1 receptor expression in the nucleus accumbens. Group housing may prevent increment of acid-induced writhing following psychosocial stress pre-exposure and effectively reduce the neuronal activity in the medial septal nucleus.
Table of contents·························································7
List of figures····························································8
Materials and Methods················································12-17
The model of social defeat /psychosocial stress··························12-13
The model of acetic acid-induced writhing test·····························13
Social interaction test·················································14
The model of marble-burying test·······································14
Fos-staining and quantification··········································15
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