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系統識別號 U0026-2207201909524400
論文名稱(中文) 社會失敗/社會心理壓力的預曝露可能透過伏隔核中TRPV1受體的上調使冰醋酸誘導的扭體增加
論文名稱(英文) Social defeat /psychosocial stress pre-exposure produces increases in the acetic acid-induced writhing possibly via upregulation of TRPV1 receptor in the nucleus accumbens
校院名稱 成功大學
系所名稱(中) 生理學研究所
系所名稱(英) Department of Physiology
學年度 107
學期 2
出版年 108
研究生(中文) 黃郁涵
研究生(英文) Yu-Han Huang
學號 S36064119
學位類別 碩士
語文別 英文
論文頁數 39頁
口試委員 指導教授-游一龍
口試委員-陳景宗
口試委員-楊尚訓
中文關鍵字 社會心理壓力  社交挫敗  群體陪伴  內臟疼痛  扭體反應 
英文關鍵字 psychosocial stress  social defeat  group housing  visceral pain  writhing responses 
學科別分類
中文摘要 重複暴露於壓力源與我們的健康風險有關。社會心理壓力可能加劇身體疼痛。然而,很少有報告指出社會心理壓力是否能調節內臟疼痛的程度。與身體疼痛不同的是,內臟疼痛是指來自於內臟器官所引發的疼痛。我們假設預先曝露社會心理壓力可能調節內臟疼痛的程度,而群體陪伴則能夠減少社會心理壓力的影響。我們利用連續六天社交挫敗的小鼠模型作為社會心理壓力,在腹腔注射冰醋酸作為模擬急性的內臟疼痛,並且以扭體反應表示內臟疼痛的程度,並且以三隻小鼠為一組作為群體陪伴觀察扭體反應。我們發現與沒有受到壓力的小鼠相比,預先曝露六天社會心理壓力確實增加了酸所誘導扭體反應的次數。此外,若有另外兩頭同樣注射冰醋酸的小鼠存在時,顯示出能夠有效減緩彼此扭體反應的次數。而全身性給予SB366791(0.25 mg/kg,一種瞬時受體電位香草酸第一類受體的拮抗劑)可以減緩預先曝露社會心理壓力所造成的扭體反應增加,但SB366791處理在未受到壓力時並不影響反應結果。給予SB366791並不會改變群體居住對於扭體反應的影響。另外,我們發現受到預先曝露社會心理壓力的小鼠在伏隔核中的TRPV1受體表現增加。最後,我們發現若有另外兩頭同樣處理的小鼠存在時,在內側隔核中的神經元活性較低。這些結果顯示預先曝露社會心理壓力可能加強酸誘導的扭體反應,並且增加了伏隔核中TRPV1受體的表現,而群體居住則可以減緩預先曝露社會心理壓力所導致的扭體反應的增加,以及顯著降低內側隔核中的神經元活性。
英文摘要 Exposure to repeated stressor is associated with the risk of our health. It has been documented that psychosocial stress may devastate the self-reported physical pain. Nevertheless, sparse reports ever address the impact of the psychosocial stress on modulating the magnitude of visceral pain. Visceral pain is used to describe pain initiating from the internal organs in viscera, which is distinct from somatic pain. This study was undertaken to assess psychosocial stress pre-exposure may modulate the magnitudes of visceral pain and group housing may diminish such the effect of psychosocial stress. Six consecutive days of social defeat mouse model were used to mimic psychosocial stress. A single intraperitoneal acetic acid injection was used to model acute visceral pain and the writhing responses were to indicate such visceral pain magnitudes. Group housing referred to observing the acid-induced writhing responses in a group of 3 mice. We found that 6-day psychosocial stress pre-exposure indeed facilitated the number of acid-induced writhing responses as compared to the mice receiving no such stress pre-exposure. Moreover, the presence of two other acid-treated conspecifics mice in this mouse model demonstrated effectively attenuate the number of writhing responses. Systemic administration (0.25 mg/kg) of SB366791, a transient receptor potential vanilloid receptor type 1 ( TRPV1 ) antagonist, prevented increment of acid-induced writhing responses following psychosocial stress exposure, although the SB366791 treatment did not affect the writhing in stress-free mice. In contrast, the SB366791 administration did not affect the group housing exerts effects against the psychosocial stress-primed increases in acid-induced writhing. Furthermore, the expression of TRPV1 receptors had increased in the nucleus accumbens on the psychosocial stress pre-exposure mice. Finally, we found that neuronal activity was lower in the medial septal nucleus in the presence of two other acid-treated conspecifics mice. Per the study, results showed that psychosocial stress pre-exposure may enhance the acid-induced writhing and increase TRPV1 receptor expression in the nucleus accumbens. Group housing may prevent increment of acid-induced writhing following psychosocial stress pre-exposure and effectively reduce the neuronal activity in the medial septal nucleus.
論文目次 中文摘要································································3
Abstract······························································4-5
誌謝····································································6
Table of contents·························································7
List of figures····························································8
Introduction··························································9-11
Materials and Methods················································12-17
Animals····························································12
Chemicals··························································12
The model of social defeat /psychosocial stress··························12-13
The model of acetic acid-induced writhing test·····························13
Social interaction test·················································14
The model of marble-burying test·······································14
Fos-staining and quantification··········································15
Western immunoblotting············································15-16
Statistical analysis·················································16-17
Results·····························································18-20
Discussion···························································21-22
References··························································23-27
Figures·····························································28-39
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