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系統識別號 U0026-2108201211334000
論文名稱(中文) 台灣中部慣行與有機茶園之土壤線蟲動態
論文名稱(英文) Dynamics of Soil Nematode Communities under Conventional and Organic Tea Gardens in Central Taiwan
校院名稱 成功大學
系所名稱(中) 生命科學系碩博士班
系所名稱(英) Department of Life Sciences
學年度 100
學期 2
出版年 101
研究生(中文) 廖宸玉
研究生(英文) Chen-Yu Liao
學號 L86994017
學位類別 碩士
語文別 英文
論文頁數 38頁
口試委員 指導教授-張松彬
召集委員-陳琦玲
口試委員-邱慈暉
中文關鍵字 茶樹  慣行農法  有機農法  線蟲群集  生物多樣性 
英文關鍵字 tea plantation  conventional agriculture  organic agriculture  nematode communities  biodiversity 
學科別分類
中文摘要 為估計農業管理方式對土壤線蟲群集結構動態的影響,本研究採集慣行農法(conventional agriculture, CA)茶園、有機農法(organic agriculture, OA)茶園的茶樹下(under the tea trees, U)與茶樹走道間(between the tea trees, B)土壤表層0-10公分的土壤進行研究。結果顯示,總線蟲豐度、食細菌類(bacterivores)線蟲豐度、植物寄生類(plant-parasites)線蟲豐度在不同處理(CA-U, CA-B, OA-U, OA-B)及不同月份間皆有顯著差異。且除一月外,CA-B的總線蟲豐度、食細菌類線蟲豐度、優勢度(dominance)顯著高於其他處理,而屬豐度(genus richness)、生物多樣性指數(Shannon index)與均勻度(evenness)則顯著低於其他處理,推測可能原因是CA-B長期大量施肥,干擾大而有大量的小桿科(Rhabditid)線蟲。不同處理及採樣時間顯著的影響富集指數(enrichment index, EI)、基底指數(basal index, BI)及通道指數(channel index, CI)。其中,OA茶園中的食物網結構性指數(structure index, SI)值顯著的高於CA。總線蟲豐度、食細菌類線蟲豐度與優勢度與pH值、碳氮比(C/N)呈現負相關;屬豐度、生物多樣性指數、均勻度及SI則與pH值呈現正相關。此結果指出OA茶園中的土壤線蟲多樣性較CA高、且食物網結構較複雜。未來應可從增加慣行、有機茶園數量,以增加樣本數著手,或與微生物功能群結構分析結合,以進一步探討農業管理方式對土壤生態系的效應。
英文摘要 To evaluate the effect of agricultural management on the dynamic of soil nematode community structure, soil samples were collected 0-10 cm depth either under the canopy of the tea trees (U) or between the tea trees (B) in conventional agriculture (CA) and organic agriculture (OA) tea gardens. The results demonstrated that significant differences in the abundance of total nematodes, bacterivores and plant-parasites were found among treatments and months. The abundance of total nematode and bacterivores density were higher in CA-B than in other treatments. The dominance was significantly higher and the genus richness, Shannon index, and evenness were lower in CA-B than other treatments except in January. This may because fertilizers were applied in CA-B and cause the extreme dominance of Rhabditid in CA-B. Treatments and time (month) effect on the values of EI, BI and CI were significant. The values of SI were significantly higher in OA than in CA. The abundance of total nematode, bacterivores and the dominance were correlated negatively with pH and C/N, and the genus richness, Shannon index, evenness and SI were correlated positively with pH. The soil nematode diversity were higher and food webs were more stable structure in organic than in conventional agriculture. To clarify the effects of agricultural management on soil biodiversity, further studies with replicate sites or factorial experiments to integrate with microbial functional community structures are needed.
論文目次 CHINESE ABSTRACT…………………………………………………………………………………I

ENGLISH ABSTRACT…………………………………………………………………………………II

ACKNOWLEDGMENTS…………………………………………………………………………………III

LIST OF TABLES……………………………………………………………………………………VI

LIST OF FIGURES…………………………………………………………………………………VII


CHAPTER

1.INTRODUCTION……………………………………………………………………………………1

2.MATERIALS AND METHODS

2.1 Description of the study site………………………………………………………4
2.2 Soil sampling and procedures…………………………………………………………5
2.3 Soil physicochemical properties……………………………………………………5
2.4 Extraction and identification
of soil nematodes………………………………………………………………………6
2.5 Calculation of nematode
ecological indices………………………………………………………………………7
2.6 Statistical analysis……………………………………………………………………7

3.RESULTS

3.1 Soil physicochemical properties……………………………………………………9
3.2 Total Abundance of soil nematodes…………………………………………………9
3.3 Nematode community structure
(trophic groups)………………………………………………………………………10
3.4 Nematode ecological indices…………………………………………………………10
3.5 Correlation between soil nematodes
and soil chemical properties………………………………………………………11

4.DISCUSSION

4.1 Comparison of soil nematode
community between conventional
and organic tea gardens………………………………………………………………13
4.2 Variation of nematode ecological indices
in conventional and organic tea gardens…………………………………………15
4.3 Correlation between soil nematodes and
soil chemical properties and ecological
significance of organic agriculture………………………………………………18

5.CONCLUSIONS……………………………………………………………………………………21

REFERENCES…………………………………………………………………………………………22

TABLE I~VII………………………………………………………………………………………28

FIGURE 1~4…………………………………………………………………………………………35
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