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系統識別號 U0026-1907201005044400
論文名稱(中文) 具加熱功能之微型化電穿孔儀的開發
論文名稱(英文) The Development of a Mini-Electroporator with Heating Function
校院名稱 成功大學
系所名稱(中) 電機工程學系專班
系所名稱(英) Department of Electrical Engineering (on the job class)
學年度 98
學期 2
出版年 99
研究生(中文) 蔡瑞安
研究生(英文) Jui-An Tsai
電子信箱 aUmbrella@gmail.com
學號 N2796126
學位類別 碩士
語文別 中文
論文頁數 95頁
口試委員 指導教授-楊明興
口試委員-婁世亮
共同指導教授-王德華
中文關鍵字 設計  微型化  脈衝  穿孔  經皮吸收   
英文關鍵字 Design  Mini  Pulse  Electroporator  Electroporation  Transdermal  Heat 
學科別分類
中文摘要 以電脈衝穿孔的方式加強藥物經皮吸收的成效,正被科學界廣泛地應用及研究當中,然而目前無論是實驗性或醫療性的電脈衝穿孔儀,皆處於大體積、重量沉、高售價的階段,且一律仰賴於室內電源插座的定點限制。有鑑於此,本研究希望能開發出一套既輕巧又廉價且由電池驅動的微型化電穿孔儀,藉以達成可攜式的目標,擺脫現有對電脈衝穿孔儀笨重且昂貴的既定框架,期使該儀器蛻變為一種極具機動性、便利性的行動式醫療或研究器材。本儀器在操作介面上除了採三鍵式的設計外,更賦予各種輔助性的功能設計,例如記憶卡的存取、實時時鐘、電池監控電路等,藉以方便使用者對系統的操作、監控與記錄,完善本儀器之主要及次要功能。儘管電脈衝穿孔法已被證實能有效強化藥物的經皮吸收率,目前各界研究人員依舊以各種物理性或化學性的輔佐方式,試圖與電脈衝穿孔法形成協同強化的效果。因此,本研究在設計微型化電脈衝穿孔儀的同時,亦嘗試加入熱輔助的功能,企圖進一步加倍強化因電脈衝穿孔而提高的藥物經皮吸收率,同時藉此補足因安全性考量而無法被提高的電脈衝電場強度。
英文摘要 The efftct of enhancing transdermal drug delivery (TDD) by electroporation (EP) is widely applied and researched in medical science. However, most of electroporators are huge, heavy, and high price for experiment or medical treatment, and also rely on fixed power supply. To solve this problem, in this thesis, we developed a light and low price mini-electroporator which is also a portable and convenient medical equipment. Besides 3-key design on user interface of the equipment, it also has some helpful function designs, for example, accessing memory card, real time clock, or circuit for monitoring batteries. All these designs can improve main and secondary functions of the equipment, and are useful when users are operating, monitoring, or recording the system.
Although EP was proved that it could enhance TDD effectively, researchers are still trying to make a synergy effect with EP by physical and chemical ways. Therefore, when designing the mini-electroporator, we try to put in a heating function and attempt to redouble TDD with EP. The heating function can also make up the limited strength of the electric pulsed field because of the security consideration.
論文目次 摘要.......................................................................................................Ⅰ
Abstract.................................................................................................Ⅱ
致謝.......................................................................................................Ⅲ
目錄.......................................................................................................Ⅳ
圖目錄....................................................................................................Ⅶ
表目錄....................................................................................................XI
第一章緒論 ............................................................................................1
1.1 開發背景...........................................................................................1
1.2 開發動機...........................................................................................2
1.3 開發目的...........................................................................................4
第二章文獻探討.......................................................................................5
2.1 電脈衝波形的定義與選用.....................................................................5
2.2 電脈衝穿孔電壓對人體皮膚的疼痛臨界值..............................................7
2.3 電脈衝穿孔合併熱輔助對藥物經皮吸收的協同強化.................................7
2.4 高溫對人體皮膚的熱傷害臨界值...........................................................8
2.5 市售電脈衝穿孔儀...............................................................................9
第三章系統設計與製作.............................................................................11
3.1 硬體架構...........................................................................................11
3.1.1 微控制器(Microcontroller) ................................................................13
3.1.2 高壓方波電脈衝電路 .......................................................................14
3.1.2.1 升壓電路(DC-DC Boost Converter)..................................................14
3.1.2.2 高壓方波電脈衝產生電路 ..............................................................20
3.1.3 溫控電路 ........................................................................................21
3.1.3.1 熱電偶與冷結點補償(Cold Junction Compensation) ..........................24
3.1.3.2 感溫與溫度補償電路 .....................................................................27
3.1.3.3 PI 軟式電熱片(Polyimide Heater) ...................................................29
3.1.3.4 加熱與溫控電路 ...........................................................................29
3.1.4 其他輔助性功能設計 ........................................................................31
3.1.4.1 安全數碼卡(Secure Digital Memory Card) .......................................35
3.1.4.2 實時時鐘(Real Time Clock) ...........................................................39
3.1.4.3 電池電壓偵測電路 ........................................................................41
3.2 軟體架構...........................................................................................42
3.2.1 「Desktop」功能設計......................................................................44
3.2.2 「Setting」功能設計........................................................................45
3.2.3 「Heating or Pulsing」與任意鍵中斷執行的功能設計..........................47
3.2.4 「Loading」功能設計.......................................................................48
3.2.5 「Time」功能設計...........................................................................49
3.2.6 隱藏式「輸出校驗用SW 電脈衝」功能設計........................................51
第四章系統測試與結果 ............................................................................52
4.1 系統介紹...........................................................................................52
4.1.1 硬體介紹 .......................................................................................53
4.1.1.1 主機與配件 .................................................................................53
4.1.1.2 使用者介面 .................................................................................54
4.1.1.3 其他硬體 ....................................................................................55
4.1.2 軟體介紹 .......................................................................................56
4.1.2.1 系統桌面 ....................................................................................56
4.1.2.2 「Set」功能................................................................................56
4.1.2.3 「Heating or Pulsing」與任意鍵中斷執行的功能.............................57
4.1.2.4 「Time」功能..............................................................................60
4.1.2.5 「Load」功能..............................................................................62
4.1.2.6 「輸出校驗用SW 電脈衝」功能....................................................64
4.2 系統測試 .........................................................................................65
4.2.1 方波電脈衝垂落(Drop)測試 .............................................................65
4.2.2 最低負載測試與限制 ......................................................................70
4.2.3 脈衝寬度之125 ns 解析度測試 ........................................................72
4.2.4 脈衝間隔測試 ................................................................................74
4.2.5 脈衝次數測試 ................................................................................74
4.2.6 溫控測試 .......................................................................................75
4.2.7 電壓或電流消耗測試 .......................................................................77
4.3 動物試驗...........................................................................................79
4.3.1 電脈衝穿孔法用以強化小鼠皮膚對甲苯胺藍的透皮吸收.......................79
4.3.1.1 材料與方法 .................................................................................80
4.3.1.2 試驗結果 ....................................................................................83
第五章討論.............................................................................................85
5.1 系統測試討論 ...................................................................................85
5.1.1 專屬於RE 應用範疇的電穿孔儀........................................................85
5.1.2 電脈衝干擾....................................................................................85
5.1.3 電脈衝穩壓期間仍處於脈衝釋出狀態.................................................86
5.1.4 個別的電極片與加熱片,無法確保其作用位置的一致性.......................87
5.2 動物試驗討論 ...................................................................................87
5.2.1 為BLAB/c 小鼠除毛的手續 ..............................................................87
5.2.2 麻醉劑於小鼠皮膚的施打位置...........................................................88
5.2.3 實驗後小鼠皮膚於顯微鏡下觀測時間過久,將導致TBO 的擴散............88
5.2.4 分子量僅有305.83 道爾頓的TBO,未能完全凸顯EP 強化TDD 的效果...88
第六章結論與未來發展.............................................................................89
6.1 結論..................................................................................................89
6.2 未來發展...........................................................................................90
參考文獻.................................................................................................92
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