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系統識別號 U0026-1811201016070400
論文名稱(中文) 芝麻酚對於壓力引發胃潰瘍之保護作用探討
論文名稱(英文) Protective effect of 3, 4-methylenedioxyphenol (sesamol) on stress-related mucosal injury in rats
校院名稱 成功大學
系所名稱(中) 環境醫學研究所
系所名稱(英) Institute of Environmental and Occupational Health
學年度 99
學期 1
出版年 99
研究生(中文) 陳一瑋
研究生(英文) Yi-Wei Chen
學號 s7697406
學位類別 碩士
語文別 中文
論文頁數 57頁
口試委員 指導教授-劉明毅
口試委員-許德榮
召集委員-游一龍
中文關鍵字 壓力相關胃潰瘍  冷水束縛壓力誘導模式  芝麻酚 
英文關鍵字 stress-related gastric ulcer  water immersion restrain stress model  sesamol 
學科別分類
中文摘要 臨床上,壓力引發之胃潰瘍對於重症病人有明顯提高死亡率的情形。芝麻酚,為芝麻油中所含有的一種木質素,具有抗氧化作用及預防藥物引發胃潰瘍,但在作用於壓力所引起的胃潰瘍目前仍未知。因此,本研究主要目的為探討芝麻酚對於壓力引發胃潰瘍的保護作用及其機轉。本研究主要分成三部份,(一)冷水浸潤束縛模式誘導壓力性胃潰瘍模式之建立,運用不同溫度、時間、保定器及社會支持作用等變項進行實驗,找出最佳化壓力性胃潰瘍的誘發模式;(二)處理芝麻酚檢驗其保護作用,口服給予不同劑量芝麻酚觀察對壓力性潰瘍誘導傷害形成之影響;(三)保護作用產生可能的機制探討,測定經處理芝麻酚及誘導壓力性潰瘍大鼠胃部的各種傷害及保護因子,如脂質過氧化、胃黏液、發炎相關物質及發炎細胞於胃中含量。研究結果顯示,以保定板束縛於25°C水溫下四小時,能誘導出穩定之壓力性潰瘍,且無明顯的社會支持作用的保護影響;芝麻酚處理下能有效降低壓力引發的潰瘍及出血情形;脂質過氧化及黏液生成並未有顯著的變化,但在發炎相關之細胞激素、轉錄因子及巨噬細胞可觀察到有明顯下降之情形。芝麻酚可能經由降低發炎前細胞激素生成而降低因壓力引發的胃黏膜傷害。
英文摘要 Patients with stress-related mucosal disease have higher morbidity and mortality than the others at intensive care unit. 3,4-Methylenedioxyphenol (sesamol) has potent antioxidative and anti-inflammatory effects. The aim of this study is to investigate the effect of sesamol on water immersion restraint (WIR)-induced stress-related mucosal disease in rats. Rat gastric ulcer and hemorrhage were induced by WIR. The validation of WIR was tested first. For protective effect of sesamol, rats were pretreated orally with various doses of sesamol (0.1, 0.3, or 1 mg/kg) 30 min before WIR. Restrained on restraint board at 25℃ for 4 h induced severe ulcer and hemorrhage. Social support effect did not affect the induction of stress ulcer. WIR increased gastric mucosal ulcer and hemorrhage, tumor necrosis factor (TNF)-, interleukin (IL)-1, and IL-6 levels, compared with non-WIR group, but failed to affect mucosal lipid peroxidation and mucus secretion. Sesamol significantly decreased gastric ulceration and hemorrhage, decreased mucosal tumor necrosis factor  (TNF-, interleukin 1IL-1, and interleukin 6 (IL-6) productions, and increased interleukin 10 (IL-10), compared with WIR control group. Sesamol inhibited nuclear factor-B (NF-B) activity in sesamol-treated groups compared with WIR control group. In addition, increased myeloperoxidase (neutrophil marker) and CD68 (macrophage marker) levels in gastric mucosa were found in WIR-treated rats. Sesamol significantly decreased CD68 levels but not myeloperoxidase in mucosa compared to WIR-control groups. We hypothesize that sesamol protects against stress-related mucosal disease by inhibiting gastric mucosal proinflammatory cytokines production in rats.
論文目次 一、 致謝……………………………………………………………………1
二、 英文摘要……………………………………………...……………….2
三、 中文摘要………………………………………………………………3
四、 研究背景 ……………………………………………………………..4
五、 研究目的 ..…………………………………………………………...10
六、 實驗設計 …………………………………………………………….10
七、 材料與方法 ………………………………………………………….11
八、 實驗結果…………………………………………………..………….17
九、 討論……………………………………………………………..…….21
十、 研究結論 …………………………………………………..………...23
十一、 參考文獻 …………………………………………………….…... 24
十二、 圖表 ………………………………………………………….……31
十三、 附件 ………………………………………………..…...…………53
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