||Miniaturized Automatic Cell Culture System
||Department of Electrical Engineering
In recent years, Cancer has become one of most dangerous disease in the word. People tend to pursue career, while ignoring the importance of health. It leads an increasing number of cancer patients. Thus, a lot of research have been put forward to analysis the viability of cells in these decades. Among those methods, electric analysis develop rapidly. However, it is a defectiveness that electric analysis chips cannot culture cells on chip while facing a long term measurement. This study proposes a miniaturized automatic cell culture system to achieve those conditions. This system provide a saturated temperature by an electric heating sheet, a PMMA cover to avoid the gas evaporate, and using the CO2 solution to supply the CO2 gas for cell chamber. This system choose micropump to deliver the medium because of its mini scale. Moreover, the medium measuring electrodes were designed into the chamber to detect the medium and transfer signal to pump driving circuit automatically. In this case, this cell culture system not only reduce the size of the entire system to realize the miniaturization but also achieve the automatic culturing. After applying this proposed system and a normal incubator to culture cells respectively for several times, there is nearly no obvious difference in cell growth.
LIST OF FIGURES VI
LIST OF TABLE VIII
CHAPTER 1 INTRODUCTION 1
1.1 Background and Motivation 1
1.2 Cell Culture 3
1.3 Tiny-scale Device 4
CHAPTER 2 DEVICE DESIGN 5
2.1 Hardware Design 5
2.1.1 PMMA Cover Design 6
2.1.2 Chamber Design 9
2.2 Electrodes Design 12
2.2.1 Interdigitated Electrodes 12
2.2.2 Medium Detector 15
2.3 Micropump 17
2.3.1 Peristaltic Pump 17
2.3.2 Driving Circuit 19
2.4 Miniaturization 23
2.5 Automation 25
CHAPTER 3 EXPERIMENTAL SETUPS 27
3.1 Chamber Fabrication 27
3.2 System Setup 30
3.3 Materials 32
3.4 Culturing Setup 34
CHAPTER 4 RESULTS AND DISCUSSION 37
4.1 Miniaturization 37
4.2 Medium Detector 39
4.3 Morphology 41
4.4 Difference of Impedance 43
CHAPTER 5 CONCLUSION AND FUTURE WORK 45
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