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系統識別號 U0026-1608201016120500
論文名稱(中文) 自組裝單層膜修飾電極在尿蛋白生醫感測之應用
論文名稱(英文) Application of Self-Assembled Monolayer Modified Electrode for Albumin Biosensing in Urine
校院名稱 成功大學
系所名稱(中) 化學工程學系碩博士班
系所名稱(英) Department of Chemical Engineering
學年度 98
學期 2
出版年 99
研究生(中文) 李佳錚
研究生(英文) Chia-Cheng Li
學號 n3697442
學位類別 碩士
語文別 中文
論文頁數 153頁
口試委員 指導教授-楊明長
口試委員-周澤川
口試委員-杜景順
口試委員-黃炳照
口試委員-許梅娟
中文關鍵字 尿蛋白  循環伏安法  交流阻抗分析法  自組裝單層膜 
英文關鍵字 Urinary Albumin  Cyclic Voltammetry  AC Impedance  Self-assembled Monolayer (SAM) 
學科別分類
中文摘要 人體尿蛋白在臨床的腎臟病診斷時扮演重要角色,尿蛋白的檢測可以協助醫護人員判斷腎臟功能及其疾病的嚴重程度。本研究中擬製備電化學尿蛋白感測器,主要利用循環伏安法及交流阻抗分析法探討不同濃度、時間及酸鹼值下Fe(CN)63- / Fe(CN)64- 系統的峰電位、峰電流以及阻抗的變化,並利用自組裝單層膜修飾金電極提高靈敏度。
實驗結果顯示,利用N-(3-Dimethylaminopropyl)-N’-ethylcarbod- iimide hydrochloride ( EDC )及N-hydroxysuccinimide ( NHS ) 穩定電極表面的修飾層是可行的,以自組裝單層膜(SAM)修飾金電極與裸電極比較,電極在修飾前後皆為不可逆電化學反應,峰電位差及峰電流比率隨著吸附時間而改變,40分鐘後趨於穩定。隨著白蛋白濃度提高,不可逆性上升、反應活性變小,峰電位差對白蛋白的靈敏度從裸金電極的6.260 mV/ppm提升到SAM電極的7.398 mV/ ppm。而峰電流比率對白蛋白的靈敏度從裸電極的1.324 ppm-1(氧化)及1.506 ppm-1(還原)提升到SAM電極的1.703 ppm-1(氧化)及1.831 ppm-1(還原)。Rs及Rct 也隨著濃度及吸附時間成上升趨勢。
英文摘要 Human urinary albumin plays an important role in the clinical diagnosis of kidney disease. The examination of urine albumin can help medical staff determine kidney function and seriousness of disease. In this study, an electrochemical urinary albumin sensor was proceduced. It mainly used cyclic voltammetry and ac impedance analysis to detect peak potential, peak current and impedance in different concentrations and adsorption times of albumin, and pHs of electrolyte in a Fe(CN)63- / Fe(CN)64- system. SAM modified electrodes were also applied to increase sensitivity of the detection.
The result shows the feasibility by using EDC and NHS to stabilize the modification layer of electrode surface. According to the results from the SAM modified and bare electrodes, the reactions on electrodes both before and after modification were all irreversible eletochemical reaction. Peak potential and peak current ratio changed with adsorption time and became stable after 40 minutes. The sensitivity of peak potential difference was 6.260 mV/ppm with bare electrode, and was improved to 7.398 mV/ ppm with SAM electrode. When albumin concentration increased, the irreversibility increased and activity of electrode decreased. The sensitivity of peak current ratio was 1.324 μA/ppm (oxidation) and 1.506 μA/ppm (reduction) with bare electrodes, and was improved to 1.703 μA/ppm (oxidation) and 1.831 μA/ppm (reduction) with SAM electrodes. Rs and Rct increased with the albumin concentration and adsorption time.
論文目次 目 錄
中文摘要 I
Abstract II
誌 謝 IV
目 錄 V
圖目錄 IX
表目錄 XII
符號說明 XIII
第一章 緒論 1
1.1 前言 1
1.2 泌尿系統的簡介 3
1.2.1 主要泌尿器官及其功能 3
1.2.2 尿液的基本介紹 3
1.2.3 尿液形成的過程 4
1.2.4 尿液檢體採集 4
1.2.5 尿液常規檢驗 4
1.3 蛋白尿簡介 7
1.3.1 何謂蛋白尿 7
1.3.2 蛋白尿與腎臟的關係 8
1.3.3 蛋白尿分析方式 9

1.4 感測器的介紹 12
1.4.1 生醫感測器 12
1.4.2 生醫感測器種類 15
1.5 文獻回顧 20
1.6 研究動機與目的 22
第二章 理論 23
2.1 電化學原理 23
2.2 電化學測定原理 25
2.2.1循環伏安法 25
2.2.2 交流阻抗分析法 31
2.3 SAM修飾電極之原理 39
2.3.1 自組裝單層膜(SAM)簡介 39
2.3.2 自組裝單層膜之種類 40
2.3.3 自組裝單層膜與裸金電極間的關係 41
第三章 實驗 44
3.1 藥品及儀器設備 44
3.1.1 實驗藥品 44
3.1.2 儀器設備 46
3.2 藥品與溶液配製 47
3.3 電極之前處理 49
3.3.1 裸電極(Bare electrode) 49
3.3.2 修飾電極(Modified electrode) 49
3.3.3 相對電極(Counter electrode) 49
3.3.4 參考電極(Reference electrode) 49
3.4 白蛋白吸附實驗 53
3.4.1 裸電極之實驗步驟 53
3.4.2 SAM電極之實驗步驟 55
第四章 結果與討論 57
4.1 裸電極 58
4.1.1 白蛋白吸附時間之影響 58
4.1.1.1 循環伏安法分析 58
4.1.1.2 交流阻抗分析 71
4.1.2 白蛋白濃度之影響 77
4.1.2.1 循環伏安法分析 77
4.1.2.2 交流阻抗分析 84
4.1.3 待測電解液pH值之影響 89
4.1.3.1 循環伏安法分析 89
4.1.3.2 交流阻抗分析 93
4.1.4 小結 97
4.2 修飾金電極 99
4.2.1 白蛋白吸附時間於SAM電極之影響 99
4.2.1.1 循環伏安法分析 99
4.2.1.2 交流阻抗分析 107
4.2.2 白蛋白濃度於SAM電極之影響 113
4.2.2.1 循環伏安法分析 113
4.2.2.2 交流阻抗分析 120
4.2.3 待測電解液pH值之影響 125
4.2.3.1 循環伏安法分析 125
4.2.3.2 交流阻抗分析 129
4.2.4 小結 133
4.3 綜合討論 134
4.3.1 金電極在修飾前後峰電位之變化 134
4.3.2 金電極在修飾前後峰電流之變化 138
4.3.3 金電極在修飾前後阻抗之變化 142
第五章 結論 145
第六章 參考文獻 147

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