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系統識別號 U0026-1607201512571600
論文名稱(中文) 常壓非熱平衡電漿處理對可產生黃麴毒素之真菌及其宿主的影響
論文名稱(英文) Effects of atmospheric pressure non-thermal plasma treatments on aflatoxigenic fungi and its host
校院名稱 成功大學
系所名稱(中) 太空與電漿科學研究所
系所名稱(英) Institute of Space and Plasma Sciences
學年度 103
學期 2
出版年 104
研究生(中文) 陳伯辰
研究生(英文) Bo-Chen Chen
學號 LA6021014
學位類別 碩士
語文別 英文
論文頁數 49頁
口試委員 口試委員-柳克強
口試委員-談永頤
指導教授-向克強
中文關鍵字 電漿處理  黃麴毒素  真菌 
英文關鍵字 plasma treatments  aflatoxin  fungi 
學科別分類
中文摘要 本實驗之目的在於試驗常壓非熱平衡態電漿處理對於預防可產生黃麴毒素之真菌感染的效用。電漿中含有帶電粒子、電場、自由基和紫外線等成分,而這些成分都有能力在非熱平衡態電漿處理時與處理表面引起不同的化學或物理效應。在本實驗中,實驗樣本接受不同時間及不同氣體組成的非直接接觸電漿處理,非直接接觸處理也就表示僅有由電漿處理所引起的遠距離效應能被觀察到。在電漿源方面,本實驗所使用之電漿是由介電質放電的方式產生。而放電中所使用的氣體為空氣和一為 97%氦氣與 3%氧氣所混和而成之氣體。在電漿處理過後,樣本之真菌感染情形可藉由照片記錄所觀察出,而產生黃麴毒素與否可藉由紫外線螢光法所做出的定性檢測確認。而實驗結果顯示出,不論是由空氣電漿或是由氦氧混和氣體電漿所產生之遠距離效應皆增強了真菌的成長速率。此同時表示,接受過非直接接觸電漿處理之樣本的真菌成長速率較未接受過電漿處理的控制組顯著。最後再由黃麴毒素定性檢測的結果來確認生長在樣本上之真菌確實是可產生黃麴毒素之真菌。
英文摘要 This experiment tests the ability of atmospheric pressure non-thermal plasma treatments in the prevention of aflatoxigenic fungi infection. There are charged particles, electric field, radicals and UV light inside plasmas and these elements might trigger different physical or chemical effects during non-thermal plasma treatments. In this experiment, the experimental samples received indirect plasma treatments with different time duration and gas compositions which mean only the remote effects caused by plasma treatments could be seen. In this work, plasmas were produced by dielectric barrier discharge method. The operation gases were air and a mixed gas of 97% He and 3% O2. After plasma treatments, fungi growth rate was observed by taking pictures and the existence of aflatoxin was qualitatively detected by black light method. The final results show that the radicals in both He/O2 and air plasma might facilitate fungi growth rate which means peanuts received indirect plasma treatments grew fungi faster than control group. The outcomes of aflatoxin detection also show that the fungi grown on all the sample are aflatoxigenic fungi.
論文目次 Abstract..............................................I
Acknowledgements....................................III
Contents.............................................IV
List of figures.......................................V
List of abbreviations...............................VII
Symbols............................................VIII
1 Introduction........................................1
2 Literature review...................................2
2.1 Basics of plasma and gas discharge...............2
2.1.1 Introduction to plasma........................2
2.1.2 Gas discharge process and plasma classification........................................3
2.2 Reviews of atmospheric pressure discharge methods..............................................10
3 Materials and Methods..............................16
3.1 Experimental device configuration...............16
3.2 Plasma treatment on peanut......................21
3.3 Fungi growing process...........................22
3.4 Aflatoxin detection methods.....................23
4 Results and discussion.............................25
4.1 Effect on fungi growth..........................25
4.2 Effect on aflatoxin production..................35
5 Conclusions........................................45
References...........................................46
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