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系統識別號 U0026-1208201323231100
論文名稱(中文) 蛋胺酸限制飲食與耐力運動對去卵巢大鼠肝臟氧化壓力與老化相關蛋白之影響
論文名稱(英文) Effects of methionine restricted diet and endurance exercise on oxidative stress and aging related protein expression in liver of ovariectomized rats
校院名稱 成功大學
系所名稱(中) 體育健康與休閒研究所
系所名稱(英) Institute of Physical Education, Health & Leisure Studies
學年度 101
學期 2
出版年 102
研究生(中文) 蔡欣蓉
研究生(英文) Shin-Jung Tsai
學號 rb6001053
學位類別 碩士
語文別 英文
論文頁數 46頁
口試委員 指導教授-黃滄海
共同指導教授-蔡秀純
口試委員-郭家驊
中文關鍵字 更年期  蛋胺酸限制  耐力運動  氧化壓力 
英文關鍵字 menopause  methionine restriction  endurance exercise  oxidative stress 
學科別分類
中文摘要 老化是人人都會經歷的生命過程,隨著年齡的增加使得細胞的活性、再生能力與代謝功能退化。而婦女在老化的過程中伴隨著雌激素減少,產生許多生理上的改變這時期又稱為更年期。停經後的婦女由於雌激素減少,導致氧化壓力增加及發炎反應。藉由非藥物治療方式,如飲食限制及運動來改善氧化壓力達到健康促進。目的:本研究為探討蛋胺酸限制飲食與耐力運動對去卵巢大鼠肝臟氧化壓力與老化相關蛋白之影響。方法:將35週Sprague-Dawley (SD)大鼠分成6組,分別為Sham (偽手術控制組), Ovx(去除卵巢組), Ovx+Exe(去除卵巢+運動組), Ovx+MR(去除卵巢+蛋胺酸限制組), Ovx+MR+Exe(去除卵巢+蛋胺酸限制組+運動),Ovx+Est (去除卵巢+雌激素之正向控制組)。運動訓練共12週,1週運動5天,速度16 m/min,時間60 min/day。動物實驗所採取的血液樣本用來進一步分析血液中老化相關指標(IGF-1, insulin)及氧化壓力指標(TBARS濃度),以及肝臟胰島素受器、IGF-1受器及磷酸化胰島素/IGF-1受器蛋白表現量。統計分析方法以one-way ANOVA進行組間考驗,P值 < 0.05時,再以Fisher's LSD進行事後比較。結果:於體重增加數據顯示Ovx+MR+Exe 組顯著低於各組,Ovx 組顯著高於各組。血液及肝臟TBARS (硫代巴比妥酸反應物質) 指標,血液中TBARS含量以Sham及Ovx 組高於其他組別;於肝臟中TBARS含量Ovx組高於Sham, Ovx+Exe 及 Ovx+MR+Exe。血液IGF-1未達顯著差異,但以Ovx組的數據最高。血清中胰島素濃度Ovx組顯著高於所有組別;葡萄糖濃度在Sham, Ovx, Ovx+Exe組高於Ovx+MR及Ovx+Est組,且Ovx+MR+Exe組高於Ovx+Est組。肝臟胰島素受器(Insulin Receptor, IR)蛋白表現在Sham組顯著高於Ovx+Est及Ovx+MR組之外的組別。而胰島素生長因子-1受器 (IGF-I Receptor, IGF-1R)、磷酸化胰島素生長因子-1受器/胰島素受器 (Phospho-IGF-I Receptor /Insulin Receptor, p-IGF-1/ IR)、蛋白質激酶B (Protein kinase B, Akt)、磷酸化蛋白質激酶B (Phospho-Protein kinase B, p-Akt)蛋白表現各組間未達顯著差異。結論:蛋胺酸限制飲食及耐力性運動均對於去除卵巢所引起體重上升、全身與局部肝臟氧化壓力指標有顯著調降作用,此可能與減緩因去卵巢導致的IGF-1和胰島素增加有關。
英文摘要 Aging, a part of life cycle, is not only the procedure of increase in calendar age, but also includes the degradation of cell activity, metabolic function and cell regeneration. For women, the deficiency of estrogen in the process of aging, which accompany with various physiologically changes called the menopause. In postmenopausal women, estrogen deficiency caused higher oxidative stress and inflammation. There are various non-pharmacological strategies, such as diet restricted and exercise, capable to reduce postmenopausal related oxidative stress and inflammation. Purpose: To determine the effects of methionine restricted (MR) diet and endurance exercise (Exe) on oxidative stress and aging related protein expression in liver of ovariectomized rats. Methods: Female Sprague-Dawley (SD) rats of 35 weeks were subjected to sham/ovariectomy (Ovx) and assigned to six groups, which were the Sham (Control group), Ovx, Ovx+Exe, Ovx+MR, Ovx+MR+Exe and Ovx+estrogen (Est) (Positive control group) groups. A treadmill exercise was set for training groups with a regime of 16 m/min for 60 min/day, 5 days per week for 12 weeks. Assays of oxidative stress and aging related protein were conducted in serum and liver tissue. One-way analysis of variance (one-way ANOVA) and Fisher's LSD method were used for data processing and post hoc comparisons, respectively. Results: In body weight gain, the Ovx+MR+Exe group and the Ovx group were significantly lower and higher than all other groups, respectively. Serum thiobarbituric acid reactive substances (TBARS) level of the Sham and Ovx groups were significantly higher than other groups. TBARS level in liver tissue was significantly higher in the Ovx group as compared to the Sham, Ovx+Exe and Ovx+MR+Exe groups. Though none significance was shown in serum IGF-1, the Ovx group revealed a marginally higher level. Serum insulin of the Ovx group was significantly higher than all the other groups; serum glucose levels of the Sham, Ovx, Ovx+Exe groups were significantly higher than the Ovx+MR and Ovx+Est groups; besides, the Ovx+MR+Exe group was higher than the Ovx+Est group. Liver insulin receptor (IR) protein expression was significantly higher in the Sham group as compared to other groups except for the Ovx+Est and Ovx+MR groups. Conclusion: MR diet and endurance exercise show efficacy in reducing the estrogen-deficiency caused body weight gain and systemic/local oxidative stress, which were associated to the mitigations of IGF-1and insulin levels.
論文目次 摘要 I
Abstract II
誌謝 III
List of Tables VI
List of Figures VII
Abbreviations VIII
Chapter 1 Introduction 1
Operational definitions 3
Chapter 2 Literature review 4
2.1 Menopause and aging 4
2.1-1 Aging and related indices 4
2.1-2 Menopause and related aging index 5
2.2 The effects of dietary MR 5
2.2-1 MR diet and antioxidant system 6
2.2-2 MR diet and IGF-1 7
2.3 The effects of endurance exercise 7
2.3-1 Endurance exercise and antioxidant system 8
2.3-2 Endurance exercise and IGF-1 8
2.4 Summary 9
Chapter 3 Materials and Methods 10
3.1 Animal 10
3.2 Experimental Design 10
3.3 Diets 10
3.4 Exercise training design 12
3.5 Blood sample collection and analyses 13
3.6 Collection and analysis of liver tissue 13
3.7 Statistical analysis 17
Chapter4 Results 18
4-1 Body weight gain and foodintake 18
4.1-1 Body weight gain 18
4.1-2 Food intake (Food intake per kilogram of body weight) 21
4.2 Analyses of TBARS 24
4.2-1 Serum TBARS 24
4.2-2 Liver TBARS 25
4.3 Analyses of serum maker 26
4.3-1 Serum IGF-1 26
4.3-2 Serum insulin 27
4.3-3 Serum glucose 28
4.4 Analyses of western blotting 29
4.4-1 IR, IGF-1 R and p-IGF-1/IR 29
4.4-2 Akt and p-Akt 30
Chapter 5 Discussion 31
5.1 Body weight gain and food intake 31
5.2 Oxidative stress: Serum and liver TBARS 32
5.3 Aging and regulate of blood sugar related indices: IGF-1and insulin 32
5.4 Aging related protein expression 34
Conclusion 35
References 36
參考文獻 Ables, G. P., Perrone, C. E., Orentreich, D., & Orentreich, N. (2012). Methionine-restricted C57BL/6J mice are resistant to diet-induced obesity and insulin resistance but have low bone density. PLoS One, 7, 1-12.
Alexander, I. M. (2012). The history of hormone therapy use and recent controversy related to heart disease and breast cancer arising from prevention trial outcomes. Journal of Midwifery and Women's Health, 57, 547-557.
Anderson, E. (2004). Cellular homeostasis and the breast. Maturitas, 48, 13-17.
Armstrong, D., & Browne, R. (1994). The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds related to oxidative stress as applied to the clinical chemistry laboratory. Advances in Experimental Medicine and Biology, 366, 43-58.
Baeza, I., Fdez-Tresguerres, J., Ariznavarreta, C., & De la Fuente, M. (2010). Effects of growth hormone, melatonin, oestrogens and phytoestrogens on the oxidized glutathione (GSSG)/reduced glutathione (GSH) ratio and lipid peroxidation in aged ovariectomized rats. Biogerontology, 11, 687-701.
Baksu, B., Davas, I., Agar, E., Akyol, A., & Uluocak, A. (2007). Do different delivery systems of estrogen therapy influence serum lipids differently in surgically menopausal women? Journal of Obstetrics and Gynaecology Research, 33, 346-352.
Balci, S. S., & Pepe, H. (2012). Effects of gender, endurance training and acute exhaustive exercise on oxidative stress in the heart and skeletal muscle of the rat. The Chinese Journal of Physiology, 55, 236-244.
Bartke, A. (2008). Impact of reduced insulin-like growth factor-1/insulin signaling on aging in mammals: novel findings. Aging Cell, 7, 285-290.
Bartke, A., Brown-Borg, H., Mattison, J., Kinney, B., Hauck, S., & Wright, C. (2001). Prolonged longevity of hypopituitary dwarf mice. Experimental Gerontology, 36, 21-28.
Baxi, D. B., Singh, P. K., Vachhrajani, K. D., & Ramachandran, A. V. (2013). Melatonin supplementation in rat ameliorates ovariectomy-induced oxidative stress. Climacteric, 16, 274-283.
Ben Ounis, O., Elloumi, M., Zouhal, H., Makni, E., Denguezli, M., Amri, M., . . . Tabka, Z. (2010). Effect of individualized exercise training combined with diet restriction on inflammatory markers and IGF-1/IGFBP-3 in obese children. Annals of Nutrition and Metabolism, 56, 260-266.
Blagosklonny, M. V. (2008). Aging: ROS or TOR. Cell Cycle, 7, 3344-3354.
Borras, C., Gambini, J., & Vina, J. (2007). Mitochondrial oxidant generation is involved in determining why females live longer than males. Frontiers in Bioscience, 12, 1008-1013.
Borras, C., Sastre, J., Garcia-Sala, D., Lloret, A., Pallardo, F. V., & Vina, J. (2003). Mitochondria from females exhibit higher antioxidant gene expression and lower oxidative damage than males. Free Radical Biology and Medicine, 34, 546-552.
Broughton, S., & Partridge, L. (2009). Insulin/IGF-like signalling, the central nervous system and aging. Biochemical Journal, 418, 1-12.
Brown-Borg, H. M., Borg, K. E., Meliska, C. J., & Bartke, A. (1996). Dwarf mice and the ageing process. Nature, 384, 33.
Caro, P., Gomez, J., Lopez-Torres, M., Sanchez, I., Naudi, A., Jove, M., . . . Barja, G. (2008). Forty percent and eighty percent methionine restriction decrease mitochondrial ROS generation and oxidative stress in rat liver. Biogerontology, 9, 183-196.
Caro, P., Gomez, J., Sanchez, I., Naudi, A., Ayala, V., Lopez-Torres, M., . . . Barja, G. (2009). Forty percent methionine restriction decreases mitochondrial oxygen radical production and leak at complex I during forward electron flow and lowers oxidative damage to proteins and mitochondrial DNA in rat kidney and brain mitochondria. Rejuvenation Research, 12, 421-434.
Cervellati, C., Bonaccorsi, G., Cremonini, E., Bergamini, C. M., Patella, A., Castaldini, C., . . . Massari, L. (2012). Bone mass density selectively correlates with serum markers of oxidative damage in post-menopausal women. Clinical Chemistry and Laboratory Medicine, 51, 333-338.
Christ, C. Y., Hunt, D., Hancock, J., Garcia-Macedo, R., Mandarino, L. J., & Ivy, J. L. (2002). Exercise training improves muscle insulin resistance but not insulin receptor signaling in obese Zucker rats. Journal of Applied Physiology, 92, 736-744.
Colpani, V., Oppermann, K., & Spritzer, P. M. (2013). Association between habitual physical activity and lower cardiovascular risk in premenopausal, perimenopausal, and postmenopausal women: a population-based study. Menopause, 20, 525-531.
Cornel, K. M., Kruitwagen, R. F., Delvoux, B., Visconti, L., Van de Vijver, K. K., Day, J. M., . . . Romano, A. (2012). Overexpression of 17 beta-hydroxysteroid dehydrogenase type 1 increases the exposure of endometrial cancer to 17beta-estradiol. The Journal of Clinical Endocrinology and Metabolism, 97, E591-E601.
Eliakim, A., & Nemet, D. (2010). Exercise training, physical fitness and the growth hormone-insulin-like growth factor-1 axis and cytokine balance. Medicine and Sport Science, 55, 128-140.
Esterbauer, H., Schaur, R. J., & Zollner, H. (1991). Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radical Biology and Medicine, 11, 81-128.
Fontana, L., Klein, S., & Holloszy, J. O. (2006). Long-term low-protein, low-calorie diet and endurance exercise modulate metabolic factors associated with cancer risk. American Journal of Clinical Nutrition, 84, 1456-1462.
Fontana, L., Weiss, E. P., Villareal, D. T., Klein, S., & Holloszy, J. O. (2008). Long-term effects of calorie or protein restriction on serum IGF-1 and IGFBP-3 concentration in humans. Aging Cell, 7, 681-687.
Hasek, B. E., Stewart, L. K., Henagan, T. M., Boudreau, A., Lenard, N. R., Black, C., . . . Gettys, T. W. (2010). Dietary methionine restriction enhances metabolic flexibility and increases uncoupled respiration in both fed and fasted states. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 299, R728-R739.
Holzenberger, M., Dupont, J., Ducos, B., Leneuve, P., Geloen, A., Even, P. C., . . . Le Bouc, Y. (2003). IGF-1 receptor regulates lifespan and resistance to oxidative stress in mice. Nature, 421, 182-187.
Huntley, A. L., & Ernst, E. (2004). Soy for the treatment of perimenopausal symptoms-a systematic review. Maturitas, 47, 1-9.
Jung, B. H., Jeon, M. J., & Bai, S. W. (2008). Hormone-dependent aging problems in women. Yonsei Medical Journal, 49, 345-351.
Junnila, R. K., List, E. O., Berryman, D. E., Murrey, J. W., & Kopchick, J. J. (2013). The GH/IGF-1 axis in ageing and longevity. Nature Reviews Endocrinology, 9, 366-376.
Kakarla, P., Vadluri, G., & Reddy Kesireddy, S. (2005). Response of hepatic antioxidant system to exercise training in aging female rat. Journal of Experimental Zoology Part A: Comparative Experimental Biology, 303, 203-208.
Kang, N. H., Hwang, K. A., Kim, T. H., Hyun, S. H., Jeung, E. B., & Choi, K. C. (2012). Induced growth of BG-1 ovarian cancer cells by 17beta-estradiol or various endocrine disrupting chemicals was reversed by resveratrol via downregulation of cell cycle progression. Molecular Medicine Reports, 6, 151-156.
Kankofer, M., Radzki, R. P., Bienko, M., & Albera, E. (2007). Anti-oxidative/oxidative status of rat liver after ovariectomy. Journal of Veterinary Medicine Series A-physiology Pathology Clinical Medicine, 54, 225-229.
Keller, C., Larkey, L., Distefano, J. K., Boehm-Smith, E., Records, K., Robillard, A., . . . O'Brian, A. M. (2010). Perimenopausal obesity. Journal of Womens Health (Larchmt), 19, 987-996.
Kiray, M., Ergur, B. U., Bagriyanik, A., Pekcetin, C., Aksu, I., & Buldan, Z. (2007). Suppression of apoptosis and oxidative stress by deprenyl and estradiol in aged rat liver. Acta Histochemica, 109, 480-485.
Kireev, R. A., Tresguerres, A. F., Vara, E., Ariznavarreta, C., & Tresguerres, J. A. (2007). Effect of chronic treatments with GH, melatonin, estrogens, and phytoestrogens on oxidative stress parameters in liver from aged female rats. Biogerontology, 8, 469-482.
Lawrence, M. C., McKern, N. M., & Ward, C. W. (2007). Insulin receptor structure and its implications for the IGF-1 receptor. Current Opinion in Structural Biology, 17, 699-705.
LeRoith, D., & Roberts, C. T., Jr. (2003). The insulin-like growth factor system and cancer. Cancer Letters, 195, 127-137.
Lespessailles, E., Jaffre, C., Beaupied, H., Nanyan, P., Dolleans, E., Benhamou, C. L., & Courteix, D. (2009). Does exercise modify the effects of zoledronic acid on bone mass, microarchitecture, biomechanics, and turnover in ovariectomized rats? Calcified Tissue International, 85, 146-157.
Lien, T. F., Hsu, Y. L., Lo, D. Y., & Chiou, R. Y. (2009). Supplementary health benefits of soy aglycons of isoflavone by improvement of serum biochemical attributes, enhancement of liver antioxidative capacities and protection of vaginal epithelium of ovariectomized rats. Nutrition and Metabolism, 6, 1-7.
Liu, J., Yeo, H. C., Overvik-Douki, E., Hagen, T., Doniger, S. J., Chyu, D. W., . . . Ames, B. N. (2000). Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants. Journal of Applied Physiology, 89, 21-28.
Lovejoy, J. C., Champagne, C. M., de Jonge, L., Xie, H., & Smith, S. R. (2008). Increased visceral fat and decreased energy expenditure during the menopausal transition. International Journal of Obesity, 32, 949-958.
Lu, T., & Finkel, T. (2008). Free radicals and senescence. Experimental Cell Research, 314, 1918-1922.
Malloy, V. L., Krajcik, R. A., Bailey, S. J., Hristopoulos, G., Plummer, J. D., & Orentreich, N. (2006). Methionine restriction decreases visceral fat mass and preserves insulin action in aging male Fischer 344 rats independent of energy restriction. Aging Cell, 5, 305-314.
Masoro, E. J. (2005). Overview of caloric restriction and ageing. Mechanisms of ageing and development, 126, 913-922.
Miller, R. A., Buehner, G., Chang, Y., Harper, J. M., Sigler, R., & Smith-Wheelock, M. (2005). Methionine-deficient diet extends mouse lifespan, slows immune and lens aging, alters glucose, T4, IGF-I and insulin levels, and increases hepatocyte MIF levels and stress resistance. Aging Cell, 4, 119-125.
Miquel, J., Ramirez-Bosca, A., Ramirez-Bosca, J. V., & Alperi, J. D. (2006). Menopause: a review on the role of oxygen stress and favorable effects of dietary antioxidants. Archives of Gerontology and Geriatrics, 42, 289-306.
Murakami, S. (2006). Stress resistance in long-lived mouse models. Experimental Gerontology, 41, 1014-1019.
Nelson, H. D. (2008). Menopause. Lancet, 371, 760-770.
Nigam, S., & Schewe, T. (2000). Phospholipase A(2)s and lipid peroxidation. Biochimica et Biophysica Acta, 1488, 167-181.
Nishida, Y., Matsubara, T., Tobina, T., Shindo, M., Tokuyama, K., Tanaka, K., & Tanaka, H. (2010). Effect of low-intensity aerobic exercise on insulin-like growth factor-I and insulin-like growth factor-binding proteins in healthy men. International Journal of Endocrinology, 2010, 1-8.
Orentreich, N., Matias, J. R., DeFelice, A., & Zimmerman, J. A. (1993). Low methionine ingestion by rats extends life span. Journal of Nutrition, 123, 269-274.
Ozgonul, M., Oge, A., Sezer, E. D., Bayraktar, F., & Sozmen, E. Y. (2003). The effects of estrogen and raloxifene treatment on antioxidant enzymes in brain and liver of ovarectomized female rats. Endocrine Research, 29, 183-189.
Perrone, C. E., Malloy, V. L., Orentreich, D. S., & Orentreich, N. (2012). Metabolic adaptations to methionine restriction that benefit health and lifespan in rodents. Experimental Gerontology, 48, 654-660.
Piche, M. E., Lemieux, S., Weisnagel, S. J., Corneau, L., Nadeau, A., & Bergeron, J. (2005). Relation of high-sensitivity C-reactive protein, interleukin-6, tumor necrosis factor-alpha, and fibrinogen to abdominal adipose tissue, blood pressure, and cholesterol and triglyceride levels in healthy postmenopausal women. American Journal of Cardiology, 96, 92-97.
Pichini, S., Ventura, R., Palmi, I., di Carlo, S., Bacosi, A., Langohr, K., . . . Zuccaro, P. (2010). Effect of physical fitness and endurance exercise on indirect biomarkers of growth hormone and insulin misuse: Immunoassay-based measurement in urine samples. Journal of Pharmaceutical and Biomedical Analysis, 53, 1003-1010.
Plaisance, E. P., Greenway, F. L., Boudreau, A., Hill, K. L., Johnson, W. D., Krajcik, R. A., . . . Gettys, T. W. (2011). Dietary methionine restriction increases fat oxidation in obese adults with metabolic syndrome. The Journal of Clinical Endocrinology and Metabolism, 96, E836-E840.
Radak, Z., Zhao, Z., Koltai, E., Ohno, H., & Atalay, M. (2012). Oxygen Consumption and Usage During Physical Exercise: The Balance Between Oxidative Stress and ROS-Dependent Adaptive Signaling. Antioxidants and Redox Signaling, 18, 1208-1246.
Riant, E., Waget, A., Cogo, H., Arnal, J. F., Burcelin, R., & Gourdy, P. (2009). Estrogens protect against high-fat diet-induced insulin resistance and glucose intolerance in mice. Endocrinology, 150, 2109-2117.
Ristow, M., & Schmeisser, S. (2011). Extending life span by increasing oxidative stress. Free Radical Biology and Medicine, 51, 327-336.
Roddam, A. W., Allen, N. E., Appleby, P., Key, T. J., Ferrucci, L., Carter, H. B., . . . Galan, P. (2008). Insulin-like growth factors, their binding proteins, and prostate cancer risk: analysis of individual patient data from 12 prospective studies. Annals of Internal Medicine, 149, 461-471.
Rosety-Rodriguez, M., Rosety, I., Fornieles-Gonzalez, G., Diaz-Ordonez, A. J., Camacho, A., Rosety, M. A., . . . Ordonez, F. J. (2012). A 6-week training program increased muscle antioxidant system in elderly diabetic fatty rats. Medical Science Monitor, 18, BR346-BR350.
Sanchez-Roman, I., Gomez, A., Perez, I., Sanchez, C., Suarez, H., Naudi, A., . . . Barja, G. (2012). Effects of aging and methionine restriction applied at old age on ROS generation and oxidative damage in rat liver mitochondria. Biogerontology, 13, 399-411.
Sanz, A., Caro, P., & Barja, G. (2004). Protein restriction without strong caloric restriction decreases mitochondrial oxygen radical production and oxidative DNA damage in rat liver. Journal of Bioenergetics and Biomembranes, 36, 545-552.
Shimokawa, I., Higami, Y., Tsuchiya, T., Otani, H., Komatsu, T., Chiba, T., & Yamaza, H. (2003). Life span extension by reduction of the growth hormone-insulin-like growth factor-1 axis: relation to caloric restriction. FASEB Journal, 17, 1108-1121.
Shimokawa, I., Higami, Y., Utsuyama, M., Tuchiya, T., Komatsu, T., Chiba, T., & Yamaza, H. (2002). Life span extension by reduction in growth hormone-insulin-like growth factor-1 axis in a transgenic rat model. American Journal of Pathology, 160, 2259-2265.
Stampfer, M. J., Colditz, G. A., Willett, W. C., Manson, J. E., Rosner, B., Speizer, F. E., & Hennekens, C. H. (1991). Postmenopausal estrogen therapy and cardiovascular disease. Ten-year follow-up from the nurses' health study. New England Journal of Medicine, 325, 756-762.
Sun, L., Sadighi Akha, A. A., Miller, R. A., & Harper, J. M. (2009). Life-span extension in mice by preweaning food restriction and by methionine restriction in middle age. Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 64, 711-722.
Taysi, S., Oztasan, N., Efe, H., Polat, M. F., Gumustekin, K., Siktar, E., . . . Gul, M. (2008). Endurance training attenuates the oxidative stress due to acute exhaustive exercise in rat liver. Acta Physiologica Hungarica, 95, 337-347.
Thompson, P. D., Buchner, D., Pina, I. L., Balady, G. J., Williams, M. A., Marcus, B. H., . . . Wenger, N. K. (2003). Exercise and physical activity in the prevention and treatment of atherosclerotic cardiovascular disease: a statement from the Council on Clinical Cardiology (Subcommittee on Exercise, Rehabilitation, and Prevention) and the Council on Nutrition, Physical Activity, and Metabolism (Subcommittee on Physical Activity). Circulation, 107, 3109-3116.
Turgut, S., Erken, H. A., Erken, G., Ayada, C., Genc, O., & Turgut, G. (2011). The effects of docosahexaenoic acid supplementation and exercise on growth hormone and insulin-like growth factor I serum levels during chronic hypoxia in rats. Journal of Basic and Clinical Physiology and Pharmacology, 22, 103-107.
Vina, J., Borras, C., Gambini, J., Sastre, J., & Pallardo, F. V. (2005). Why females live longer than males: control of longevity by sex hormones. Science of Aging Knowledge Environment, 2005, pe17.
Vina, J., Sastre, J., Pallardo, F. V., Gambini, J., & Borras, C. (2006). Role of mitochondrial oxidative stress to explain the different longevity between genders: protective effect of estrogens. Free Radical Research, 40, 1359-1365.
Wang, H. L., Tai, M. K., Hung, H. M., & Chen, C. H. (2012). Unique symptoms at midlife of women with osteoporosis and cardiovascular disease in Taiwan. Menopause, 20, 315-321.
Warburton, D. E., Nicol, C. W., & Bredin, S. S. (2006). Health benefits of physical activity: the evidence. Canadian Medical Association Journal, 174, 801-809.
Weitzmann, M. N., & Pacifici, R. (2006). Estrogen deficiency and bone loss: an inflammatory tale. Journal of Clinical Investigation, 116, 1186-1194.
Weroha, S. J., & Haluska, P. (2012). The insulin-like growth factor system in cancer. Endocrinology and Metabolism Clinics of North America, 41, 335-350.
Wieczorek-Baranowska, A., Nowak, A., Michalak, E., Karolkiewicz, J., Pospieszna, B., Rutkowski, R., . . . Pilaczynska-Szczesniak, L. (2011). Effect of aerobic exercise on insulin, insulin-like growth factor-1 and insulin-like growth factor binding protein-3 in overweight and obese postmenopausal women. Journal of Sports Medicine and Physical Fitness, 51, 525-232.
Wilcox, G. (2005). Insulin and insulin resistance. Clinical Biochemist Reviews, 26, 19.
Wu, G., Fang, Y. Z., Yang, S., Lupton, J. R., & Turner, N. D. (2004). Glutathione metabolism and its implications for health. Journal of Nutrition, 134, 489-492.
Yagi, K. (1998). Simple assay for the level of total lipid peroxides in serum or plasma. Methods in Molecular Biology, 108, 101-106.
Yang, J., Anzo, M., & Cohen, P. (2005). Control of aging and longevity by IGF-I signaling. Experimental Gerontology, 40, 867-872.
Zimmerman, J. A., Malloy, V., Krajcik, R., & Orentreich, N. (2003). Nutritional control of aging. Experimental Gerontology, 38, 47-52.
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