系統識別號 U0026-1208201323231100
論文名稱(中文) 蛋胺酸限制飲食與耐力運動對去卵巢大鼠肝臟氧化壓力與老化相關蛋白之影響
論文名稱(英文) Effects of methionine restricted diet and endurance exercise on oxidative stress and aging related protein expression in liver of ovariectomized rats
校院名稱 成功大學
系所名稱(中) 體育健康與休閒研究所
系所名稱(英) Institute of Physical Education, Health & Leisure Studies
學年度 101
學期 2
出版年 102
研究生(中文) 蔡欣蓉
研究生(英文) Shin-Jung Tsai
學號 rb6001053
學位類別 碩士
語文別 英文
論文頁數 46頁
口試委員 指導教授-黃滄海
中文關鍵字 更年期  蛋胺酸限制  耐力運動  氧化壓力 
英文關鍵字 menopause  methionine restriction  endurance exercise  oxidative stress 
中文摘要 老化是人人都會經歷的生命過程,隨著年齡的增加使得細胞的活性、再生能力與代謝功能退化。而婦女在老化的過程中伴隨著雌激素減少,產生許多生理上的改變這時期又稱為更年期。停經後的婦女由於雌激素減少,導致氧化壓力增加及發炎反應。藉由非藥物治療方式,如飲食限制及運動來改善氧化壓力達到健康促進。目的:本研究為探討蛋胺酸限制飲食與耐力運動對去卵巢大鼠肝臟氧化壓力與老化相關蛋白之影響。方法:將35週Sprague-Dawley (SD)大鼠分成6組,分別為Sham (偽手術控制組), Ovx(去除卵巢組), Ovx+Exe(去除卵巢+運動組), Ovx+MR(去除卵巢+蛋胺酸限制組), Ovx+MR+Exe(去除卵巢+蛋胺酸限制組+運動),Ovx+Est (去除卵巢+雌激素之正向控制組)。運動訓練共12週,1週運動5天,速度16 m/min,時間60 min/day。動物實驗所採取的血液樣本用來進一步分析血液中老化相關指標(IGF-1, insulin)及氧化壓力指標(TBARS濃度),以及肝臟胰島素受器、IGF-1受器及磷酸化胰島素/IGF-1受器蛋白表現量。統計分析方法以one-way ANOVA進行組間考驗,P值 < 0.05時,再以Fisher's LSD進行事後比較。結果:於體重增加數據顯示Ovx+MR+Exe 組顯著低於各組,Ovx 組顯著高於各組。血液及肝臟TBARS (硫代巴比妥酸反應物質) 指標,血液中TBARS含量以Sham及Ovx 組高於其他組別;於肝臟中TBARS含量Ovx組高於Sham, Ovx+Exe 及 Ovx+MR+Exe。血液IGF-1未達顯著差異,但以Ovx組的數據最高。血清中胰島素濃度Ovx組顯著高於所有組別;葡萄糖濃度在Sham, Ovx, Ovx+Exe組高於Ovx+MR及Ovx+Est組,且Ovx+MR+Exe組高於Ovx+Est組。肝臟胰島素受器(Insulin Receptor, IR)蛋白表現在Sham組顯著高於Ovx+Est及Ovx+MR組之外的組別。而胰島素生長因子-1受器 (IGF-I Receptor, IGF-1R)、磷酸化胰島素生長因子-1受器/胰島素受器 (Phospho-IGF-I Receptor /Insulin Receptor, p-IGF-1/ IR)、蛋白質激酶B (Protein kinase B, Akt)、磷酸化蛋白質激酶B (Phospho-Protein kinase B, p-Akt)蛋白表現各組間未達顯著差異。結論:蛋胺酸限制飲食及耐力性運動均對於去除卵巢所引起體重上升、全身與局部肝臟氧化壓力指標有顯著調降作用,此可能與減緩因去卵巢導致的IGF-1和胰島素增加有關。
英文摘要 Aging, a part of life cycle, is not only the procedure of increase in calendar age, but also includes the degradation of cell activity, metabolic function and cell regeneration. For women, the deficiency of estrogen in the process of aging, which accompany with various physiologically changes called the menopause. In postmenopausal women, estrogen deficiency caused higher oxidative stress and inflammation. There are various non-pharmacological strategies, such as diet restricted and exercise, capable to reduce postmenopausal related oxidative stress and inflammation. Purpose: To determine the effects of methionine restricted (MR) diet and endurance exercise (Exe) on oxidative stress and aging related protein expression in liver of ovariectomized rats. Methods: Female Sprague-Dawley (SD) rats of 35 weeks were subjected to sham/ovariectomy (Ovx) and assigned to six groups, which were the Sham (Control group), Ovx, Ovx+Exe, Ovx+MR, Ovx+MR+Exe and Ovx+estrogen (Est) (Positive control group) groups. A treadmill exercise was set for training groups with a regime of 16 m/min for 60 min/day, 5 days per week for 12 weeks. Assays of oxidative stress and aging related protein were conducted in serum and liver tissue. One-way analysis of variance (one-way ANOVA) and Fisher's LSD method were used for data processing and post hoc comparisons, respectively. Results: In body weight gain, the Ovx+MR+Exe group and the Ovx group were significantly lower and higher than all other groups, respectively. Serum thiobarbituric acid reactive substances (TBARS) level of the Sham and Ovx groups were significantly higher than other groups. TBARS level in liver tissue was significantly higher in the Ovx group as compared to the Sham, Ovx+Exe and Ovx+MR+Exe groups. Though none significance was shown in serum IGF-1, the Ovx group revealed a marginally higher level. Serum insulin of the Ovx group was significantly higher than all the other groups; serum glucose levels of the Sham, Ovx, Ovx+Exe groups were significantly higher than the Ovx+MR and Ovx+Est groups; besides, the Ovx+MR+Exe group was higher than the Ovx+Est group. Liver insulin receptor (IR) protein expression was significantly higher in the Sham group as compared to other groups except for the Ovx+Est and Ovx+MR groups. Conclusion: MR diet and endurance exercise show efficacy in reducing the estrogen-deficiency caused body weight gain and systemic/local oxidative stress, which were associated to the mitigations of IGF-1and insulin levels.
論文目次 摘要 I
Abstract II
誌謝 III
List of Tables VI
List of Figures VII
Abbreviations VIII
Chapter 1 Introduction 1
Operational definitions 3
Chapter 2 Literature review 4
2.1 Menopause and aging 4
2.1-1 Aging and related indices 4
2.1-2 Menopause and related aging index 5
2.2 The effects of dietary MR 5
2.2-1 MR diet and antioxidant system 6
2.2-2 MR diet and IGF-1 7
2.3 The effects of endurance exercise 7
2.3-1 Endurance exercise and antioxidant system 8
2.3-2 Endurance exercise and IGF-1 8
2.4 Summary 9
Chapter 3 Materials and Methods 10
3.1 Animal 10
3.2 Experimental Design 10
3.3 Diets 10
3.4 Exercise training design 12
3.5 Blood sample collection and analyses 13
3.6 Collection and analysis of liver tissue 13
3.7 Statistical analysis 17
Chapter4 Results 18
4-1 Body weight gain and foodintake 18
4.1-1 Body weight gain 18
4.1-2 Food intake (Food intake per kilogram of body weight) 21
4.2 Analyses of TBARS 24
4.2-1 Serum TBARS 24
4.2-2 Liver TBARS 25
4.3 Analyses of serum maker 26
4.3-1 Serum IGF-1 26
4.3-2 Serum insulin 27
4.3-3 Serum glucose 28
4.4 Analyses of western blotting 29
4.4-1 IR, IGF-1 R and p-IGF-1/IR 29
4.4-2 Akt and p-Akt 30
Chapter 5 Discussion 31
5.1 Body weight gain and food intake 31
5.2 Oxidative stress: Serum and liver TBARS 32
5.3 Aging and regulate of blood sugar related indices: IGF-1and insulin 32
5.4 Aging related protein expression 34
Conclusion 35
References 36
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