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系統識別號 U0026-0812200915335464
論文名稱(中文) 電生理與光學評估腦缺血大鼠之神經血管反應
論文名稱(英文) Electrophysiological and Optical Assessment of Neurovascular Coupling in Rat with Cerebral Ischemia
校院名稱 成功大學
系所名稱(中) 醫學工程研究所碩博士班
系所名稱(英) Institute of Biomedical Engineering
學年度 97
學期 2
出版年 98
研究生(中文) 連英淇
研究生(英文) Ying-chi Lien
電子信箱 p8696119@mail.ncku.edu.tw
學號 p8696119
學位類別 碩士
語文別 英文
論文頁數 45頁
口試委員 指導教授-陳家進
口試委員-鄭國順
口試委員-王德華
口試委員-葉秩光
口試委員-勞勃特律格
中文關鍵字 神經血管連結  近紅外光譜儀  缺血性中風  體感覺誘發電位 
英文關鍵字 ischemic stroke  near infrared spectroscopy  neurovascular coupling  somatosensory evoked potential 
學科別分類
中文摘要 為了研究缺血性中風模型下大腦功能失常,我們需要在急性期做持續且即時的量測,才能夠對於腦組織的存亡提供重要資訊。本研究結合電生理與光學影像系統來評估大鼠在腦缺血前後對於前腳電刺激的神經血管反應。儀器的部分包含了電刺激器以誘發體感覺皮質,電生理參數的放大器與紀錄系統,近紅外光譜儀以量測血流動力學的變化。我們設計了一個放置在大腦上的探頭,可以把電極與光纖排在定位,讓電訊號和光訊號同時被擷取。在系統驗證的階段,我們進行了健全的活體實驗去確認現有的機構能同時獲得體感覺誘發電位與事件相關的光訊號。接著我們做了一個引導性的動物實驗以檢視神經反應與血流反應對於重複刺激的時程變化,並且將神經血管連結的正常模式跟短暫頸動脈阻塞後的變異做比較。結果發現在腦缺血的傷害下,導致神經和血管反應的強度都有20至50%的衰減,而在誘發反應到尖峰的時間也有延遲的現象。總結來說,利用神經血管連結的評估將有助於非侵入監測大腦在局部缺血後的應變性與功能恢復。
英文摘要 For systemic study of cerebral dysfunction under ischemic stroke, continuous and instant measurement is crucial to provide the information about the eventual fate of brain tissue in the acute stage. This study combined electrophysiological measurement and optical imaging system for assessment of neurovascular responses to forepaw stimulation in rat model before and after experimental cerebral ischemia. The instrumentation contains an electrical stimulator to evoke somatosensory cortex, a biopotential amplifier to measure electrophysiological change, a near infrared spectroscopy to measure hemodynamic change. We designed a custom-made cranial probe which aligned electrodes and optic fibers for concurrent electrical and optical signal acquisition. At the stage of system validation, robust in-vivo experiments had been performed to verify the availability of somatosensory evoked potentials (SEP) and event related optical signals (EROS). We have conducted a pilot animal experiment to investigate the temporal evolution of neural response and hemodynamic response to iterative stimulations. The pattern of neurovascular coupling in normal was compared with the altered pattern after transient carotid artery occlusion. Both neuronal and vascular response showed 20-50% decay and delay in time-to-peak response due to ischemic insult. In conclusion, the utilization of neurovascular coupling assessment could help to investigate the noninvasive monitoring of cerebral reactivity and functional recovery after experimental focal ischemia.
論文目次 中文摘要 .................................. i
Abstract ................................. ii
誌謝......................................iii
Contents...................................iv
List of Tables.............................vi
List of Figures............................vi

Chapter1 Introduction ................................... 1
1.1 Pathophysiology and evaluation of stroke ............ 1
1.2 Physiological basis of neurovascular coupling ....... 2
1.3 Brain activity measurements ......................... 3
1.4 Application of optical imaging on neurovascular coupling ............................................... 10
1.5 Deviation of neurovascular response after ischemic stroke ................................................. 11
1.6 Motivations and purposes of this study ............. 12

Chapter 2 Materials and Methods ........................ 14
2.1 Instrumentation .................................... 14
2.1.1 Measurement of SEP ............................... 14
2.1.2 Optical imaging .................................. 17
2.2.3 Integrated cranial probe ......................... 21
2.2 Experimental design ................................ 23
2.2.1 Animal preparation ............................... 23
2.2.2 Measurement of neurovascular responses ........... 24
2.2.3 Cerebral ischemia ................................ 25
2.3 Data analysis ...................................... 25
2.3.1 SEP .............................................. 25
2.3.2 NIRS ............................................. 26

Chapter 3 Results ...................................... 27
3.1 In-vivo trials for instrumentational validation .... 27
3.1.1 SEP measurement .................................. 27
3.1.2 Optical imaging .................................. 30
3.2 Baseline response of neurovascular coupling ........ 31
3.3 Neurovascular response under focal cerebral ischemia 35

Chapter 4 Discussion and Conclusion .................... 38
References ............................................. 41
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