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系統識別號 U0026-0812200915205279
論文名稱(中文) 運動訓練對第一型糖尿病鼠周邊神經病變之成效
論文名稱(英文) Effects of Treadmill Exercise on Peripheral Neuropathy in Type 1 Diabetic Rats
校院名稱 成功大學
系所名稱(中) 物理治療研究所
系所名稱(英) Department of Physical Therapy
學年度 97
學期 2
出版年 98
研究生(中文) 謝佩伶
研究生(英文) Pei-ling Hsieh
電子信箱 t6696404@mail.ncku.edu.tw
學號 t6696404
學位類別 碩士
語文別 英文
論文頁數 58頁
口試委員 口試委員-鄭瑞棠
口試委員-陳郁文
指導教授-洪菁霞
中文關鍵字 糖尿病神經病變  熱休克蛋白72  運動訓練 
英文關鍵字 HSP72  diabetic neuropathy  exercise training 
學科別分類
中文摘要 研究背景與目的:糖尿病患常見的併發症其中一項便是糖尿病周邊神經病變。研究顯示熱休克蛋白72 是具有神經保護特性,而且在正常老鼠體內若經運動訓練可以誘發出熱休克蛋白。因此本研究希望以運動介入的方式來了解是否在糖尿病鼠的周邊神經也可以誘發熱休克蛋白72,並改善周邊神經病痛的症狀。研究方法:將Wistar 的公鼠分為四組:未訓練正常組、正常運動組、未訓練糖尿病組、糖尿病運動組。在誘發糖尿病後第三天開始運動介入,一直持續到第十四天、第二十八天或第五十六天。運動鼠一週跑五天,每天跑30-60 分鐘,每次以20-25 公尺/分鐘的速度進行。周邊神經病變的症狀則是藉以測量觸覺敏感的von Frey filaments 和痛覺敏感的plantar test
來表示。熱休克蛋白72 的表現量則是以西方墨點法來測量。結果:糖尿病運動組的老鼠在觸覺敏感與痛覺敏感的部分均有顯著改善,但經觀察此效果無法長期維持。熱休克蛋白72 在周邊神經的表現量則較未訓練糖尿病組來的多。結論:運動訓練有助於糖尿病周邊神經病變症狀的改善,而此情形可能與運動在周邊神經誘發出熱休克蛋白72 有關。
英文摘要 Background and Purpose: Diabetic patients suffer from many complications, and one of them is diabetic peripheral neuropathy (DPN). As heat shock protein 72 (HSP72) has the
neuroprotective function and can be induced from normal rats with exercise training. We hypothesize that exercise may induce HSP72 overexpression in peripheral nerves and
reduce the symptoms of DPN in streptozotocin-induced diabetic rats. Methods: Adult male Wistar rats were randomly assigned to one of the following groups: normal sedentary group, normal exercise group, and diabetic rats with/without exercise training groups.
Training started from day 3 after inducement until day 14, day 28 or day 56. The trained rats run on a treadmill 5 days/week, 30-60 min/day at 20-25m/min. We use von Frey
filaments and plantar test to evaluate the symptoms of DPN and compare the difference between each group. In addition, HSP72 expressions in peripheral nerves were determined
by western blot with monoclonal anti-HSP72 antibody. Results: There were significant improvements in tactile allodynia and thermal hyperalgesia in diabetic rats with exercise training. However, the effects on allodynia can not maintain for long term. HSP72 expressions in spinal cord, sciatic nerve and peripheral nerves were significantly greater in diabetic rats with exercise training than those without exercise. Conclusions: Exercise
training conferred significant improvements in the symptoms of DPN and that could be correlated with the overexpression of HSP72 in peripheral nerves
論文目次 Abstract ------------------------------------------------------------------------1
Chinese abstract---------------------------------------------------------------3
Acknowledge------------------------------------------------------------------4
List of figures------------------------------------------------------------------6
Abbreviation-------------------------------------------------------------------8
Introduction--------------------------------------------------------------------9
Methods-------------------------------------------------------------------------13
Results---------------------------------------------------------------------------17
Discussion-----------------------------------------------------------------------19
Reference------------------------------------------------------------------------27
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