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系統識別號 U0026-0812200915065645
論文名稱(中文) 以微衛星DNA基因序列探討葉芽阿拉伯芥之族群遺傳結構
論文名稱(英文) Population structuring of Arabidopsis halleri ssp. gemmifera based on microsatellite DNAs
校院名稱 成功大學
系所名稱(中) 生物多樣性研究所
系所名稱(英) Institute of Biodiversity
學年度 97
學期 1
出版年 98
研究生(中文) 郭怡君
研究生(英文) Yi-chun Kuo
電子信箱 L8693103@mail.ncku.edu.tw
學號 L8693103
學位類別 碩士
語文別 中文
論文頁數 45頁
口試委員 口試委員-李亞夫
口試委員-宋皇模
指導教授-蔣鎮宇
中文關鍵字 葉芽阿拉伯芥  遺傳分化  族群結構  微衛星序列  遺傳變異 
英文關鍵字 genetic variation  genetic divergence  population structuring  microsatellite DNA  Arabidopsis halleri ssp. gemmifera 
學科別分類
中文摘要 葉芽阿拉伯芥是模式植物阿拉伯芥的近緣種,兩者大約在五百萬年前分化。相較於阿拉伯芥為一年生且自花授粉,葉芽阿拉伯芥為多年生、異花授粉,且對重金屬也有較高的耐受性。
隨著分子生物技術的進步,生物學者利用演化上屬中性遺傳標誌的微衛星序列來了解族群結構及遺傳變異。本研究利用8組微衛星引子分析來自台灣雪山和南湖大山、中國長白山及日本京都,共16族群,138個葉芽阿拉伯芥DNA樣本。結果發現遺傳變異主要存在族群內的個體間,達90.75%;同一地理區的族群間具有9.22%。然而,地理區間的遺傳變異卻很低,只有0.03%。推論可能因為葉芽阿拉伯芥族群棲地多半在岩坡及岩縫中因而限制地下莖生長範圍分佈在1~3公尺之間,加上種子的傳播會受到重力影響而限制傳播距離,所以造成族群內個體間的遺傳變異高。但葉芽阿拉伯芥的花粉會透過大黃蜂、小蜜蜂、蚜蠅和蝴蝶等昆蟲傳播較遠的距離,特別是大黃蜂和蝴蝶更能將花粉傳播數百公里以上的距離而形成地理區間的基因流動。因此,不同地理區的族群間亦有遺傳變異但地理區間的遺傳變異卻非常低。
英文摘要 Arabidopsis halleri ssp. gemmifera is one of the closest wild relatives of Arabidopsis thaliana, with an estimated divergence time of ~5million years. A. halleri ssp. gemmifera is a perennial outcrossing plant and is tolerant to heavy metals, whereas A.thaliana is an annual selfing species. Biologists use microsatellites DNA, a neutral genetic marker, to assess population structuring and genetic variation. In this study, I analysed 138 DNA samples of 16 populations of A. halleri ssp. gemmifera, which are distributed in Taiwan, Northeast China, and Japan, based on 8 microsatellites primers. Genetic analyses revealed that 90.75% of genetic varation was distributed within populations, while 9.22% among populations within groups. In contrast, very little genetic differentiation, 0.03% of variation, was detected among groups. It was propose that habitats of A. halleri ssp. gemmifera are rocky slopes and crevices to limite distributions of rhizomatous ramates between 1 and 3 meters. Limited dispersal distances for small, gravity-dispersaed seeds was probably one of the reasons, too. Therefore, genetic varation within populations was high. In another way, bumblebees, small bees, syrphids and butterflies can disperse the pollens of A. halleri ssp. gemmifera in the long distances, especially bumblebees and butterflies can disperse hundreds of kilometers. As a result, there was genetic variation among populations within groups but very little genetic differentiation among groups.
論文目次 中文摘要…………………………………………………………………….…I
英文摘要…………………………………………………………………....….II
誌謝…………………………………………………………………….………III
目錄………………………………………………………………………….....IV
表目錄……………………………………………………………………….....V
圖目錄……………………………………………………………………….....VI
第壹章 前言
一、族群遺傳學……………………………………….……………………...1
二、微衛星DNA的簡介……………………………………………………...1
三、微衛星序列的優點…………………………………………………..…..2
四、阿拉伯芥的重要性……………………………………………………....3
五、葉芽阿拉伯芥(Arabidopsis halleri ssp. gemmifera)………………….6
六、為何研究阿拉伯芥的近緣物種………………………………………....8
七、研究目的………………………………………………………………....8
第貳章 材料與方法
一、研究材料………………………………………………………….……...9
二、研究方法………………………………………………………………....9
三、資料分析………………………………………………………………..11
第參章 結果
一、遺傳變異以及遺傳結構分析….……………………………………….13
二、族群變動分析…………………………………………………………..13
三、族群結構分析……………………………...………………………….14
第肆章 討論
一、葉芽阿拉伯芥之遺傳變異………………………………………….….15
二、不同地理區間族群分化…………………………………………….….16
三、廣泛的基因流動範圍……………………………………………….….17
四、遺傳關係樹圖分析………………………………………………….….17
五、族群結構分析……………………………………………………….….18
第伍章 結論…………………………………………………………….. …..19
第陸章 參考文獻………………………………………………………..…...20
表……………………………………………………………………….….....25
圖…………………………………………………………………….…….....42
自述……………………………………………………………….……….....45
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