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系統識別號 U0026-0812200913470196
論文名稱(中文) 整合式電化學尿蛋白感測晶片系統
論文名稱(英文) Integrated Microfluidic System for Electrochemical Sensing of Urine Proteins
校院名稱 成功大學
系所名稱(中) 工程科學系碩博士班
系所名稱(英) Department of Engineering Science
學年度 95
學期 2
出版年 96
研究生(中文) 劉政祐
研究生(英文) Cheng-yu Liu
學號 n9694124
學位類別 碩士
語文別 中文
論文頁數 78頁
口試委員 指導教授-李輝煌
指導教授-李國賓
口試委員-周澤川
中文關鍵字 微幫浦  微閥門  疏水閥門  微流體系統  微機電系統 
英文關鍵字 micropump  microvalve  hydrophobic valve  MEMS  microfluidics 
學科別分類
中文摘要 本研究成功地結合微流體技術及電化學感測方法在一微流體晶片中來偵測尿蛋白。此微流體晶片由自動檢體稀釋晶片模組與尿蛋白檢測晶片模組組成。自動檢體稀釋晶片模組由疏水閥門、氣動式閥門與微流管道所構成,主要負責在實驗時調配所需的檢體濃度;尿蛋白檢測晶片模組則由氣動式環形幫浦、微流管道與尿蛋白感測晶片組成,負責檢體的傳輸與尿蛋白的偵測。
在晶片製作方面,二模組所需的微流體控制元件包含微流管道、疏水閥門、氣動式閥門、氣動式環形幫浦,皆以聚二甲基矽氧烷(Polydimethylsiloxane, PDMS)材料製作,而感測電極係利用電聚合方法在白金電極上電聚合吡咯(pyrrole, PY)和胺苯硼酸(aminophenylboronic acid, APBA)薄膜完成,最後再利用氧電漿將上板微流體晶片與感測電極封裝完成。
  研究中利用十倍的磷酸鹽緩衝液(Phosphate Buffer Saline, PBS)(pH=5)來模擬人體尿液,並以白蛋白做為感測目標物,運用此微流體感測晶片做快速偵測,並觀察溶液中是否有尿蛋白的存在,以判斷人體的健康情況。研究之最終目標是希望藉由此系統的發展,提供一個快速檢測的平台技術。
英文摘要 This paper presents a new microfluidic system capable of detecting urine proteins in an automatic fashion. The system integrated with two major functional devices including a sample dilution module and a urine protein detection module. The sample dilution module consisting of a hydrophobic valve, microvalves and microchannels is designed for diluting the concentration of protein samples. The urine protein detection module is composed of a new spiral-shape micropump, microchannels and an electrochemical sensor for urine protein detection. This module is used for transporting the protein sample solution and for detecting the urine proteins inside the solution. All of the microfluidic structures in the system are fabricated by using soft lithography of polydimethylsiloxane (PDMS). Then, the electrode for the urine protein sensor is formed by depositing and patterning a thin-film of platinum (Pt), followed by electro-depositing a thin polypyrrole layer and poly-aminophenylboronic acid to form the sensing layer. Finally, the sample dilution module and the urine protein detection module are bonded together using the oxygen plasma treatment to form an integrated system. Besides, the proposed integrated system can successfully detect the human urine proteins with a detection limit of 0.1 ppm, As a whole, this study may provide a powerful platform for electrochemical detection of urine proteins.
論文目次 目 錄

摘 要 i
Abstract ii
誌 謝 iv
目 錄 vi
表 目 錄 ix
圖 目 錄 x
縮 寫 說 明 xiii
符 號 說 明 xiv

第一章 緒論
1-1 前言 1
1-2 生醫微機電系統簡介 2
1-3 生物感測器簡介 4
1-4 微流體技術與生物感測器之整合 5
1-5 研究動機與目的 6
1-6 研究方法 8
1-7文獻回顧 10
1-7-1 微幫浦文獻回顧 10
1-7-2 微閥門文獻回顧 12
1-7-3 電化學感測器 14
1-8論文架構 16

第二章 晶片設計
2-1 晶片動作流程 18
2-2 微流體檢測系統 20
2-2-1 環形蠕動式微氣動幫浦與微閥門原理探討 20
2-2-2 尿蛋白感測電極 23
2-2-3 微流體操控系統晶片設計 24
2-2-4 微流體檢測系統之操作原理 27
2-3 濃度稀釋系統 29
2-3-1 疏水閥門原理探討 30
2-3-2 濃度稀釋系統晶片設計 33
2-3-3 濃度稀釋系統之操作原理 35

第三章 晶片製作
3-1感測底板製作 37
3-2微流體元件製作 40
3-3接合與封裝 45

第四章 實驗架設
4-1 實驗裝置與架設 49
4-2 尿蛋白感測電極之製備 51
第五章 結果與討論
5-1 微氣動幫浦和微閥門效能測試 52
5-2 濃度稀釋系統的效能測試 60
5-3 尿蛋白感測電極測試實驗 63

第六章 結論與未來展望
6-1 結論 69
6-2 未來展望 70

參 考 文 獻 71
自     述 76
著     作 77
參考文獻 參考文獻

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