進階搜尋


 
系統識別號 U0026-0812200912113815
論文名稱(中文) 利用定量高壓液態層析質譜儀大範圍的分析在心肺傷害中雌激素接受器Beta所調控的蛋白質表現
論文名稱(英文) Large Scale Analysis of Estrogen Receptor Beta-mediated Proteins by Quantitative LC/MS/MS in Heart and Lung Injury
校院名稱 成功大學
系所名稱(中) 生理學研究所
系所名稱(英) Department of Physiology
學年度 94
學期 2
出版年 95
研究生(中文) 陳永祥
研究生(英文) Yun-Hsiang Chen
電子信箱 doskoi168@yahoo.com.tw
學號 s3692112
學位類別 碩士
語文別 英文
論文頁數 80頁
口試委員 口試委員-陳淑慧
指導教授-蔡美玲
口試委員-蘇五洲
中文關鍵字 心肺  高壓液態層析質譜儀  定量  雌激素接受器Beta 
英文關鍵字 Estrogen Receptor Beta  Quantitative LC/MS/MS  Heart  Lung 
學科別分類
中文摘要 在生物體內,女性荷爾蒙雌激素(estrogen)對於所作用的器官系統具有很廣泛的影響。雌激素的作用是透過兩種不同的雌激素接受器(estrogen receptor)來影響生物體的功能。這兩種雌激素接受器分別是雌激素接受器alpha和雌激素接受器beta。先前的研究已經指出,在雌激素機接受器beta表現佔優勢的系統中,像是心臟和肺臟。雌激素扮演著很重要的腳色,影響著心肺的功能。但是雌激素在心肺系統的詳細作用並不是非常清楚。因此,本實驗將利用蛋白質體學的分析方法來大範圍的分析雌激素對於心肺系統的影響。
本實驗中,利用心肌梗塞和經由超細微碳粒所誘發的肺發炎的動物模式來探討雌激素接受器beta在心肺傷害中所扮演的腳色。藉由雌激素接受器beta的促效劑genistein和DPN來活化雌激素接受器,以便分析雌激素接受器所扮演的腳色。並且結合一維膠電泳與液態層析串聯是質譜儀來分析在心肺傷害中雌激素接受器所調控的蛋白質變化。
實驗結果顯示,在這兩個動物模式中,雌激素機接受器beta可以減少因為發炎而引起的能量代謝增加。本研究藉由蛋白質體學的方法提供了一個新的觀點,雌激素接受器beta在心肺受到傷害時,可以抑制能量的代謝,來達到保護心肺作用的功能。




英文摘要 The female steroidal hormone estrogen triggers a wide variety of biological effects in a number of target systems. The effects of estrogen are mediated by 2 different nuclear hormone receptors, ERα and ERβ.
Previous study reported that estrogen play an important role in ERβ dominant systems, heart and lung. But the effects of ERβ on heart and lung are ont all clear. Proteomics approaches provide a large scale analysis of ERβ-mediated proteins in cardiopulmonary system.
In this study, we use the myocardial infarction model and ultra fine carbon black (ufCB)-induced lung inflammation model. Estrogen receptor beta agonists, DPN and genistein, are applied to analyze the effect of ERβ on this two models. And 1-D gel combined with quantitative LC/MS/MS is applied to analyze the estrogen receptor beta mediated proteins alteration in the two models. In the result, quantitative LC/MS/MS reveals that estrogen receptor β agonist decrease energy metabolism proteins expression in this two models. This study provides a new viewpoint. Estrogen receptor β may repress the upraised energy metabolism after stress in heart and lung.




論文目次 誌謝..................................................1
中文摘要..............................................2
Abstract..............................................3
Introduction..........................................4
Aims..................................................7
Materials and methods.................................8
Results..............................................13
Discussion...........................................22
References...........................................28
Figures..............................................36
Tables...............................................48
Curriculum vitae.....................................80
參考文獻 Baker AE, Brautigam VM, Watters JJ.
Estrogen Modulates Microglial Inflammatory Mediator Production via Interactions with Estrogen Receptor beta
Endocrinology. 2004 Nov;145(11):5021-32. Epub 2004 Jul 15.

Bjornstrom L, Sjoberg M.
Mechanisms of estrogen receptor signaling: convergence of genomic and nongenomic actions on target genes
Mol Endocrinol. 2005 Apr;19(4):833-42. Epub 2005 Feb 3.

Brancaccio D, Tetta C, Gallieni M, Panichi V.
Inflammation,CRP, calcium overload and a high calcium-phosphate product
Nephrol Dial Transplant. 2002 Feb;17(2):201-3.

Brown DM, Stone V, Findlay P, MacNee W, Donaldson K.
Increased inflammation and intracellular calcium caused by ultrafine carbon black is independent of transition metals or other soluble components
Occup Environ Med. 2000 Oct;57(10):685-91.

Chang CC, Chiu HF, Wu YS, Li YC, Tsai ML, Shen CK, Yang CY.
The Induction of Vascular Endothelial Growth Factor by Ultrafine Carbon
Black Contributes to the Increase of Alveolar-Capillary Permeability
Environ Health Perspect. 2005 Apr;113(4):454-60.

Chu SH, Goldspink P, Kowalski J, Beck J, Schwertz DW.
Effect of estrogen on calcium-handling proteins, β-adrenergic receptors, and function in rat heart
Life Sci. 2006 Apr 7; [Epub ahead of print]

Fontaine J, Herchuelz A, Deberg E, Pochet R, Famaey JP.
Calcium movements in aortae from arthritic rats.
Int J Tissue React. 1986;8(4):295-301.

Fukano Y, Yoshimura H, Yoshida T.
Heme oxygenase-1 gene expression in human alveolar epithelial cells (A549) following exposure to whole cigarette smoke on a direct in vitro exposure system
Exp Toxicol Pathol. 2006 Jul;57(5-6):411-8. Epub 2006 May 15.

Graeber GM, Clagett GP, Wolf RE, Cafferty PJ, Harmon JW, Rich NM.
Alterations in serum creatine kinase and lactate dehydrogenase. Association with abdominal aortic surgery, myocardial infarction and bowel necrosis
Chest. 1990 Mar;97(3):521-7.

Green S, Walter P, Kumar V, Krust A, Bornert JM, Argos P, Chambon P.
Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A
Nature. 1986 Mar 13-19;320(6058):134-9

Gulbis B, Unger P, Lenaers A, Desmet JM, Ooms HA.
Mass concentration of creatine kinase MB isoenzyme and lactate dehydrogenase isoenzyme 1 in diagnosis of perioperative myocardial infarction after coronary bypass surgery.
Clin Chem. 1990 Oct;36(10):1784-8.

Gutteridge JM.
Antioxidant properties of the proteins caeruloplasmin, albumin and transferrin. A study of their activity in serum and synovial fluid from patients with rheumatoid arthritis
Biochim Biophys Acta. 1986 Jan 30;869(2):119-27

Hartley DM, Kurth MC, Bjerkness L, Weiss JH, Choi DW.
Glutamate receptor-induced 45Ca2+ accumulation in cortical cell culture correlates with subsequent neuronal degeneration.
J Neurosci. 1993 May;13(5):1993-2000.

Hsu JL, Huang SY, Chow NH, Chen SH.
Stable-Isotope Dimethyl Labeling for Quantitative Proteomics
Anal Chem. 2003 Dec 15;75(24):6843-52.

Korte T, Fuchs M, Arkudas A, Geertz S, Meyer R, Gardiwal A, Klein G, Niehaus M, Krust A, Chambon P, Drexler H, Fink K, Grohe C.
Female mice lacking estrogen receptor beta display prolonged ventricular repolarization and reduced ventricular automaticity after myocardial infarction
Circulation. 2005 May 10;111(18):2282-90. Epub 2005 May 2.

Krause EG, Will H, Bohm M, Wollenberger A.
The assay of glycogen phosphorylase in human blood serum and its application to the diagnosis of myocardial infarction
Clin Chim Acta. 1975 Jan 20;58(2):145-54.

Kuiper GG, Enmark E, Pelto-Huikko M, Nilsson S, Gustafsson JA.
Cloning of a novel receptor expressed in rat prostate and ovary
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5925-30.

Mattson MP.
Calcium as sculptor and destroyer of neural circuitry.
Exp Gerontol. 1992;27(1):29-49.

Nilsen J, Diaz Brinton R.
Mechanism of estrogen-mediated neuroprotection: Regulation of mitochondrial calcium and Bcl-2 expression
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2842-7. Epub 2003 Feb 25.

Pelzer T, Loza PA, Hu K, Bayer B, Dienesch C, Calvillo L, Couse JF, Korach KS, Neyses L, Ertl G.
Increased mortality and aggravation of heart failure in estrogen receptor-beta knockout mice after myocardial infarction
Circulation. 2005 Mar 29;111(12):1492-8. Epub 2005 Mar 21.

Reimer KA, Ideker RE.
Myocardial ischemia and infarction: anatomic and biochemical substrates for ischemic cell death and ventricular arrhythmias
Hum Pathol. 1987 May;18(5):462-75.

Sattari S, Dryden WF, Eliot LA, Jamali F.
Despite increased plasma concentration, inflammation reduces potency of calcium channel antagonists due to lower binding to the rat heart
Br J Pharmacol. 2003 Jul;139(5):945-54.

Sidney S, Petitti DB, Quesenberry CP Jr.
Myocardial infarction and the use of estrogen and estrogen-progestogen in postmenopausal women.
Ann Intern Med. 1997 Oct 1;127(7):501-8

Smith PJ, Ornatsky O, Stewart DJ, Picard P, Dawood F, Wen WH, Liu PP, Webb DJ, Monge JC.
Effects of estrogen replacement on infarct size, cardiac remodeling, and the endothelin system after myocardial infarction in ovariectomized rats
Circulation. 2000 Dec 12;102(24):2983-9.

Solakidi S, Psarra AM, Nikolaropoulos S, Sekeris CE.
Estrogen receptors alpha and beta (ERalpha and ERbeta) and androgen receptor (AR) in human sperm: localization of ERbeta and AR in mitochondria of the midpiece.
Hum Reprod. 2005 Dec;20(12):3481-7. Epub 2005 Aug 25.

Speyer CL, Rancilio NJ, McClintock SD, Crawford JD, Gao H, Sarma JV, Ward PA.
Regulatory effects of estrogen on acute lung inflammation in mice
Am J Physiol Cell Physiol. 2005 Apr;288(4):C881-90.

Strehlow K, Rotter S, Wassmann S, Adam O, Grohe C, Laufs K et al.
Modulation of Antioxidant Enzyme Expression and Function by Estrogen
Circ Res. 2003 Jul 25;93(2):170-7. Epub 2003 Jun 19.

Titov VN, Krauze EG, Filippov IK, Rabich G, Filippova VA.
Glycogen phosphorylase activity in acute myocardial infarction
Kardiologiia. 1983 Mar;23(3):51-5.

Tiwari BS, Belenghi B, Levine A.
Oxidative Stress Increased Respiration and Generation of Reactive Oxygen Species, Resulting in ATP Depletion, Opening of Mitochondrial Permeability Transition, and
Programmed Cell Death
Plant Physiol. 2002 Apr;128(4):1271-81.

Vyhlidal C, Li X, Safe S.
Estrogen regulation of transferrin gene expression in
MCF-7 human breast cancer cells
J Mol Endocrinol. 2002 Dec;29(3):305-17.

Wang X, Simpkins JW, Dykens JA, Cammarata PR.
Oxidative Damage to Human Lens Epithelial Cells in Culture: Estrogen Protection of Mitochondrial Potential, ATP, and Cell Viability
Invest Ophthalmol Vis Sci. 2003 May;44(5):2067-75.

Yang SH, Liu R, Perez EJ, Wen Y, Stevens SM Jr, Valencia T, Brun-Zinkernagel AM, Prokai L, Will Y, Dykens J, Koulen P, Simpkins JW.
Mitochondrial localization of estrogen receptor beta
Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4130-5. Epub 2004 Mar 15.
論文全文使用權限
  • 同意授權校內瀏覽/列印電子全文服務,於2009-08-30起公開。
  • 同意授權校外瀏覽/列印電子全文服務,於2016-08-30起公開。


  • 如您有疑問,請聯絡圖書館
    聯絡電話:(06)2757575#65773
    聯絡E-mail:etds@email.ncku.edu.tw