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系統識別號 U0026-0812200911393120
論文名稱(中文) 針刺治療促進幼鼠海馬結構在Dexamethasone傷害後神經細胞增生的研究
論文名稱(英文) Study of Acupuncture Enhance Cell Proliferation in Dexamethasone Induced Hippocampal Damage of Neonatal Rat
校院名稱 成功大學
系所名稱(中) 細胞生物及解剖學研究所
系所名稱(英) Institute of Cell Biology and Anatomy
學年度 93
學期 2
出版年 94
研究生(中文) 汪宏南
研究生(英文) Hung-Nan Wang
電子信箱 T9692105@ccmail.ncku.edu.tw
學號 t9692105
學位類別 碩士
語文別 中文
論文頁數 85頁
口試委員 指導教授-簡基憲
口試委員-柯妙華
口試委員-傅毓秀
指導教授-張永賢
口試委員-林其和
中文關鍵字 針刺  海馬迴 
英文關鍵字 acupuncture  hippocampus 
學科別分類
中文摘要   合成的醣皮質類固醇(Dexamethasone, Dex)在新生兒臨床上常被用來促進早產兒肺部的成熟以降低呼吸器的使用時間、抑制肺部的炎症反應,並降低合併症的發生率,以提高存活率。Dex的副作用除了造成高血壓、腸穿孔、心室肥大、代謝異常等外,最近的研究指出接受類固醇治療的早產兒會造成大腦灰質及大腦容量的減少,身高較矮、體重較輕、頭圍較小,並出現神經功能的異常。在動物實驗方面,接受高劑量類固醇治療之老鼠或猴子的海馬結構有明顯細胞數目減少及傷害的現象。本實驗室證實Dex給藥傷害新生兒神經系統的發育,給予產後一日齡的老鼠單一高劑量的Dex即可造成海馬迴及齒狀迴細胞的傷害。針灸是傳統醫學中的一種治療方式,近年來在動物實驗或臨床上均證實針灸的療效。有更多的研究證實針灸足三里可以促進腦部缺血及糖尿病鼠海馬區細胞的增生。足三里是足陽明胃經的第36個腧穴(ST-36),位於膝下三寸,長總趾伸肌與前脛骨肌間,距脛骨一橫指處。本實驗的目的在於探討針刺足三里能否改善醣皮質類固醇對早產兒所造成的神經傷害。於產後一日齡幼鼠腹腔注射單一高劑量的Dex,於不同的日齡起 (第3、7天)針刺足三里以探討海馬迴細胞再生的能力。以5-Bromo-2’-deoxyuridine (BrdU)作為細胞增生的指標,結果顯示產後3日齡及7日齡起針刺治療的幼鼠其細胞增生的數目明顯的比未針刺組多。以Neuronal nuclei (NeuN)和Glial fibrillary acidic protein (GFAP)區別增生的前驅細胞是否分化成神經細胞或神經膠細胞,結果顯示各組前驅細胞分化成神經細胞或神經膠細胞星狀細胞的比率並無統計上明顯的差異。顯示針刺治療的確可以改善Dex造成海馬迴神經細胞的傷害,促進前驅細胞的增生。但無法決定新增的前驅細胞分化成神經細胞或神經膠細胞的機制。
英文摘要  Synthetic glucocorticoid-dexamethasone, have been widely used to accelerate pulmonary maturation and inhibit lung inflammation of preterm infants. However, follow-up studies showed that preterm infants received steroid therapy result in neurologic dysfunction, less cortical gray tissue and total brain volume. In our pilot studies, dexamethasone injected postnatal day one rat pups either with high (0.5 mg/kg) or low dose (0.2 mg/kg) result in the cellular irregularity, dendritic atrophy, increased number of pyknotic cells and vascularity in the hippocampus. The pathological changes can be observed predominantly in the CA1 subregion and the granular cell layer of dentae gyrus. Acupuncture has been applied in clinical treatment of various diseases in Oriental medicine. Growing evidence shows acupuncture enhance cell proliferation in dentate gyrus either in the global ischemic gerbil, in maternally separates stressed rats, or in the diabetic rat. Zusanli (ST-36), an acupoint located 3 mm lateral and distal to the anterior tubercle of the tibia, has been examined in various studies and has been proved to have various effects, including efficacy in the treatment of cerebrovascular diseases in humans and animals. Therefore, purpose of the study is to evaluate the effect of acupuncture at ST-36 enhanced the cell proliferation of dexamethasone damaged neonatal hippocampal formation. Through the 5-bromo-2’-deoxyuridine (BrdU) immunocytochemic stainning, our data show that dexamethasone inhibited cell proliferation and an increased number in the dentate gyrus of rats after acupuncture has been observed. The phenotype of BrdU positive cells were examined by double-labeling with the neuronal marker-NeuN, and astrocyte marker-GFAP. The percentages of cells colocalized with BrdU and NeuN in each group shows no virtually difference. The results will provide solid evidence to support that acupuncture treatment can improve the glucocorticoid damaged neuronal tissue. However the differentiation of precursor cells into neuron or glia cell needs further investigation.
論文目次 一、 中文摘要…………………………………4
二、 英文摘要…………………………………6
三、 緒論………………………………………8
四、 材料與方法………………………………11
五、 結果………………………………………26
六、 討論………………………………………32
七、 結論………………………………………46
八、 圖與表說明………………………………47
九、 參考文獻…………………………………77
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