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系統識別號 U0026-0812200911144765
論文名稱(中文) 長期純音處理對年輕大白鼠下丘核區抑制性GABAA受體功能之影響作用
論文名稱(英文) Effects of Prolonged Pure Tone Exposure on Inhibitory GABAA Receptor Functions in the Inferior Colliculus of Young Rats
校院名稱 成功大學
系所名稱(中) 生理學研究所
系所名稱(英) Department of Physiology
學年度 92
學期 2
出版年 93
研究生(中文) 賴維斌
研究生(英文) Wei-Bin Lai
學號 s3690401
學位類別 碩士
語文別 中文
論文頁數 71頁
口試委員 指導教授-吳豐森
召集委員-潘偉豐
口試委員-莊季瑛
中文關鍵字 長期純音處理  下丘 
英文關鍵字 GABAA receptor  inferior colliculus 
學科別分類
中文摘要   下丘是聽覺訊息傳遞路徑中一個重要的轉換站,其功能包括與語言相關訊息的處理、聲音定位及頭部對聲音刺激的轉向反應。有許多的實驗證據顯示,下丘核區的神經細胞對聽覺反應的敏感度很容易受到外來聲音刺激的影響而產生持久性的改變,例如:先前電生理的研究指出,大白鼠在出生後7天到14天接受一頻率為4 k Hz,聲音強度為65 dB SPL之長期純音處理後,中腦下丘核區單一聽神經對聲音有反應的最低閾值會提高及最敏感的聲音頻率為4 k Kz的細胞數目會增加。有證據顯示,抑制性GABA調控機制可能是造成上述改變的重要因素。除了突觸前GABA的釋放量可能受到改變外,突觸後GABAA受體的性質(如GABAA受體對GABA的敏感度及神經類固醇對GABAA受體的調控作用)也有可能會受到聲音處理的影響而產生持久性的變化。本研究的主要目的就是在利用全細胞膜電位固定的電生理技術和藥理學的方法,來研究長期純音處理(以4 k Hz, 65 dB SPL的純音,自老鼠出生後第7天至第14天連續處理7天)對GABA濃度反應曲線及類固醇(如:PS和5α3α)調控GABAA受體能力的影響作用,我們所用的材料為從大白鼠腦幹切片快速分離出來的下丘核區神經細胞。我們發現,長期純音處理會降低GABA的EC10-EC30值和Hill係數,但對GABA的最大反應及EC50則無影響作用,此結果顯示長期純音處理會增強低濃度GABA所誘發的電流反應。此外,長期純音處理對PS抑制GABAA受體的能力並無顯著作用,但會顯著增強5α3α對GABAA受體所媒介電流的促進作用。本研究證實在下丘核區神經細胞上之GABAA受體的性質,經過長期純音處理後,會產生持久性的改變,這些改便可能會提高對聲音有反應的最低閾值,也可能會使得神經細胞對重複聲音的刺激產生適應的現象。

英文摘要   The inferior colliculus (IC) is a major auditory midbrain structure that is critical for speech-related information processing, sound localization and head reflex to sound. Increasing evidence indicates that IC neurons are very plastic to sound manipulation. For instance, previous electrophysiological studies have shown that in adult IC more neurons are found near the frequency of a priming tone (4 kHz, 65 dB SPL) presented to the animal during the neonatal period and their minimum thresholds are elevated. Currently the GABA-mediated inhibitory neural mechanism has been implicated in sharpening the response properties of IC neurons, which may be reflected in localized changes in properties of the GABAA receptor such as its sensitivity to GABA and its modulation by neurosteriods. Using the whole-cell voltage-clamp technique and pharmacological method, the major goal of this study is thus to determine the effect of prolonged pure tone exposure (4 kHz, 65 dB SPL for 7 days form postnatal day 7 to 14) on the GABA concentration-response curve and the activity of neurosteriods such as pregnenolone sulfate (PS) and 5α-pregnan-3α-ol-20-one (5α3α to modulate the GABA-induced current in acutely dissociated rat IC neurons. Results revealed that sound treatment decreased the GABA EC10-EC30 and the Hill coefficient with little effect on the GABA maximal response and EC50, indicating the enhancing effect of sound exposure on the response induced by lower concentrations of GABA. In addition, prolonged pure tone exposure had no effect on the inhibition by PS of the GABA-induced current. However, the same sound treatment dramatically enhanced the activity of 5α3α to positively modulate the GABAA receptor. Taken together, our results support the idea that the properties of GABAA receptors in IC neurons are subject to plastic changes by prolonged pure tone exposure, which may cause the IC neurons to elevate their minimum thresholds and to become habituated after repeated sound exposure.

論文目次 中文摘要...................................Ⅰ
英文摘要...................................III
緒論....................................... 1
材料與方法................................. 7
結果....................................... 18
討論....................................... 42
參考文獻................................... 51
附錄....................................... 58
誌謝....................................... 63
履歷....................................... 64
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