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系統識別號 U0026-0812200910444629
論文名稱(中文) 以Melatonin治療暫時性局部腦缺血白鼠有助其電生理及神經行為之改善
論文名稱(英文) Delayed Treatment with Melatonin Improves Electrophysiological and Neurobehavioral Outcomes in Rats Subjected to Transient Focal Cerebral Ischemia
校院名稱 成功大學
系所名稱(中) 醫學工程研究所碩博士班
系所名稱(英) Institute of Biomedical Engineering
學年度 91
學期 2
出版年 92
研究生(中文) 蔡宜殷
研究生(英文) Yi-Yin Tsai
電子信箱 demaratw@yahoo.com.tw
學號 p8690101
學位類別 碩士
語文別 中文
論文頁數 49頁
口試委員 指導教授-張冠諒
口試委員-張志涵
口試委員-李宜堅
口試委員-鄭國順
中文關鍵字 體感覺誘發電位  電生理  褪黑激素  中風 
英文關鍵字 SSEPs  melatonin  electrophysiology  stroke 
學科別分類
中文摘要 一般而言,腦部動脈因梗塞、栓塞或其他因素突然停止供血時,會引發細胞內鈣離子的堆積,間接使得自由基的產生而導致細胞膜的破壞,最後造成不可逆性的細胞損傷及神經行為功能上之障礙。因此以一具神經保護功效之保護劑進行治療則為一重要之程序。
而褪黑激素Melatonin在近年來的研究報告中已被指出為一有效直接的自由基清除者,及一間接之抗氧化劑。而在研究中亦指出Melatonin針對腦部局部缺血進行治療,發現可降低因缺血所造成之腦部損傷。在此篇研究中,以具神經保護效能之劑量(5 mg/kg)於再灌流同時進行股靜脈注射。除了可達成降低腦部梗塞之程度外,更著眼於電生理以及神經行為上的測量及評估。因為以臨床的觀點,對於中風病人而言,其行為功能上的復原狀況相對而言較治療所降低之腦部損傷程度更為重要。
從我們的結果顯示,Melatonin不僅可減少因大腦缺血-再灌流所造成之腦梗塞體積,且對於缺血但未受治療之控制組白鼠,其於缺血側上肢及下肢皮質記錄之體感覺誘發電位( SSEPs )振幅,降至原始值之39%及36%。相對而言,以Melatonin治療之白鼠其振幅則可明顯提升至65%及74%,並且於神經行為功能上亦有明顯進步之結果。
英文摘要 In general, cerebral ischemia elicits calcium accumulation and production of free radicals leading to irreversible cell damage and neurobehavioral dysfunction. Thus an effective neuroprotective agent is needed to protect ischemic brain after thrombolytic therapies.

Melatonin is well known a direct free radical scavenger and an indirect antioxidant, and was reported to reduce infarct volume recently. In the current study, we investigate weather delayed treatment with melatonin (5mg/kg iv) could improve electrophysiology and neurobehavioral function in the transient cerebral ischemia model. Because for stroke patients through treatment, the improvement of functional outcome is more important than reduced brain damage

In controls, the amplitude of somatosensory evoked potentials (SSEPs) recorded from fore- and hindpaw cortical fields decreased to 39% and 36% of baselines. Respectively, the amplitude of SSEPs recorded from cerebral ischemia rats treat with melatonin improved to 65% and 74% of baselines. Additionally, melatonin also reduced cerebral infarction volume. In conclusion, delayed treatment with melatonin could improve the electrophysiology and neurobehavioral outcomes and reduce brain damage induced by brain ischemia and reperfusion.
論文目次 中文摘要--------------------------------------------------I
英文摘要-------------------------------------------------II
總目錄--------------------------------------------------III
圖目錄----------------------------------------------------V
表目錄--------------------------------------------------VII

第一章 緒論
1-1腦血管疾病的概論-----------------------------1
1-1-1腦血管循環的病理生理學---------------------2
1-2 抗氧化劑------------------------------------7
1-2-1 抗氧化劑在腦血管疾病上的功效--------------7
1-2-2 抗氧化劑melatonin-------------------------7
1-3 電生理學的神經傳遞信號監測-----------------11
1-3-1動作電位傳導------------------------------11
1-3-2體感誘發電位(Cortical-SSEP)---------------13
1-4研究動機及目的------------------------------17
第二章 實驗方法與步驟
2-1實驗流程------------------------------------19
2-2-1 動物的準備,麻醉以及參數的監測-----------18
2-2-2 體感覺誘發電位(SSEPs)之記錄--------------19
2-2-3 中腦動脈阻塞手術(MCA occlusion)----------24
2-2-4鼠隻的犧牲及量化缺血所造成傷害------------26
2-3神經學檢查----------------------------------27
2-4 統計分析-----------------------------------28
第三章 結果
3-1 生理參數記錄之結果----------------------29
3-2體感覺誘發電位SSEPs之記測-----------30
3-3神經行為之評估結果------------------34
3-4 腦梗塞大小之比較--------------------------35
第四章 討論
4-1 體感覺誘發電位與腦損傷程度之相關性-----------36
4-2神經行為的評估--------------------------------38
4-3 Melatonin之神經保護效果----------------------39
4-4就實驗程序探討影響誘發電位記測之因素----------40
第五章 結論與未來展望
5-1 結論-----------------------------------------42
5-2未來展望--------------------------------------43
參考文獻-----------------------------------------44
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