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系統識別號 U0026-0812200910240319
論文名稱(中文) 雌二醇對重症癲癇成年雌鼠 學習與記憶的影響作用
論文名稱(英文) Effect of 17b-Estradiol on Learning and Memory in Adult Female Rats after Status Epilepticus
校院名稱 成功大學
系所名稱(中) 生理學研究所
系所名稱(英) Department of Physiology
學年度 90
學期 2
出版年 91
研究生(中文) 高碧蓮
研究生(英文) Pi-Lang Kao
電子信箱 lingene@seed.net.tw
學號 s3688101
學位類別 碩士
語文別 中文
論文頁數 66頁
口試委員 口試委員-莊季瑛
口試委員-黃阿敏
指導教授-吳豐森
中文關鍵字 重症癲癇  學習與記憶  雌二醇 
英文關鍵字 learning and memory  17B-estradiol  status epilepticus 
學科別分類
中文摘要 許多的研究證據顯示,雌二醇(E2)對人和實驗動物的學習與記憶能力有增強的作用。亦有文獻報告指出在大腦顳葉海馬迴區域產生病變所造成的癲癇病患中,其學習與記憶能力較正常人差。我們實驗室先前的研究顯示,由kainic acid所誘發的癲癇大白鼠之學習與記憶能力較正常大白差,但由pilocarpine所誘發的癲癇大白鼠之學習與記憶能力是否也較正常大白鼠差,且E2對此種癲癇大白鼠的學習與記憶能力是否也如對正常大白鼠一樣具有增強作用則不甚清楚。在本研究中,我們利用被動逃避學習的測試方法,探討腹腔注射pilocarpine所誘發的癲癇大白鼠與正常大白鼠之學習與記憶能力是否有差異。同時,我們也研究E2對此二組動物之學習記憶能力的影響作用。成年雌鼠於兩側卵巢切除後第十天,腹腔注射pilocarpine以誘發重症癲癇40或60分鐘。我們將大白鼠分成五組:正常組、癲癇組、癲癇前E2處理組、癲癇後E2處理組及癲癇前後E2處理組,於誘發重症癲癇七天後,以被動逃避學習箱來測試並比較各組的學習與記憶能力。學習與記憶能力越好的大白鼠,訓練後,由亮室跑入暗室的時間(retention latency)越長。結果顯示重症癲癇40或60分鐘的大白鼠之retention latency顯著較正常大白鼠短,表示重症癲癇大白鼠之學習與記憶能力較正常大白鼠差。經由rotarod treadmills及電刺激敏感度的測試,得知重症癲癇40或60分鐘之大白鼠的retention latency之所以較短,並不是因為其活動能力較好或是對電刺激較不敏感所導致。此外,我們發現重症癲癇40分鐘之大白鼠海馬迴各區域無明顯受損,但重症癲癇60分鐘之大白鼠的海馬迴CA1區域則嚴重受損,表示此區域神經元的受損可能是導致重症癲癇60分鐘之大白鼠的學習與記憶能力變差的原因。雖然E2連續給予九天可顯著增強去卵巢大白鼠的學習與記憶能力,但是所有各種E2的處理(包括前處理、後處理及前後處理)對重症癲癇40或60分鐘之大白鼠受損的學習與記憶皆沒有預防或改善的效果。令人驚奇地,E2前處理、後處理或前後處理皆可使重症癲癇60分鐘之大白鼠海馬迴CA1區域的神經元保持完整無損。
英文摘要 Accumulating evidence from a variety of human and experimental animal studies indicates that 17b estradiol (E2) may act as a memory enhancer. Memory deficits, particularly deficits of long term memory, have often been reported in patients with temporal lobe epilepsy. Previous studies from our laboratory demonstrate that memory deficits also occur in kainic acid-induced seizure rats. However, whether memory impairments are also present in pilocarpine-induced seizure rats and whether E2 treatments can prevent or reverse memory impairments in seizure rats remain largely unknown. In present study, we used the one-way inhibitory avoidance learning task to explore the effect of intraperitoneally injected pilocarpine on rat memory performance. In addition, we also investigated the effect of E2 treatments on memory retention in normal and pilocarpine-induced seizure rats. Ten days after ovariectomy, the adult female rats were injected intraperitoneally with pilocarpine (400 mg/kg) to induce prolonged (40- or 60-min) seizures (status epilepticus). Seven days later, memory performance in normal, seizure, E2 pretreatment, E2 posttreatment and E2 pretreatment plus posttreatment rats was measured and compared. Results revealed that the retention latency in 40- or 60-min seizure rats was shorter than that in normal rats, indicating poorer memory performance in seizure rats. Rotarod treadmill performance and electric shock sensitivity test excluded the possibility that the shorter retention latency in 40- or 60-min seizure rats was due to their higher motor activity or lower shock sensitivity. Moreover, whereas 40-min seizures had no damaging effect on hippocampal neurons, 60-min seizures produced extensive neuronal damage in rat hippocampal CA1 area. These results suggest that neuronal loss in hippocampal CA1 area might be the cause of memory deficits in 60-min seizure rats. E2 treatment for 9 days increased memory performance in normal rats. However, all E2 treatments, including pretreatment, posttreatment and pretreatment plus posttreatment, did not prevent or reverse memory deficits in pilocarpine-induced seizure (40 or 60 min) rats. Unexpectedly, E2 pretreatment, posttreatment or pretreatment plus posttreatment prevented or reversed pilocarpine-induced neuronal damage in the hippocampal CA1 area of 60-min seizure rats.
論文目次 中文摘要…………………………………. I
英文摘要………………………………… III
緒論……………………………………… 1
材料與方法……………………………… 6
結果……………………………………… 21
討論……………………………………… 49
參考文獻………………………………… 54
附錄……………………………………… 61
致謝……………………………………… 65
履歷……………………………………… 66
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