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系統識別號 U0026-0712201012043000
論文名稱(中文) 探討奈米碳球之生物相容性
論文名稱(英文) The Biocompatibility of Carbon Nanocapsules
校院名稱 成功大學
系所名稱(中) 醫學工程研究所碩博士班
系所名稱(英) Institute of Biomedical Engineering
學年度 99
學期 2
出版年 100
研究生(中文) 林鼎圍
研究生(英文) Ting-Wei Lin
學號 P8697402
學位類別 碩士
語文別 英文
論文頁數 48頁
口試委員 指導教授-謝清河
指導教授-張志涵
口試委員-王盈錦
中文關鍵字 奈米碳球  生物相容性  奈米材料 
英文關鍵字 Carbon nanomaterials  biocompatibility  nanoparticles 
學科別分類
中文摘要 奈米碳材近年來因其特殊之結構,電學特性以及能加以不同之表面修飾, 使其廣泛地被研究於各種領域,包括了生物醫學之應用。也因此,其安全性及可能之毒性也漸漸成為熱門之議題並充滿爭議,在各種奈米碳材中,奈米碳球因為其特殊且精細的結構及尺寸,使其有潛力成為最具有生物醫療應用的成員。在本研究中,我將利用不同之分散劑製備奈米碳球溶液,並完整且系統性的利用體外及體內實驗研究奈米碳球之生物相容性。結果顯示,利用聚乙烯醇分散之奈米碳球於體外實驗中具有最低之細胞毒性且並不會造成明顯之細胞免疫反應,利用靜脈注射聚乙烯醇分散之奈米碳球至小鼠體內後亦沒有產生顯著之不良反應。本實驗證明奈米碳球為具有相當不錯生物相容性之生醫材料,未來應可以進一步應用於生物醫學領域。
英文摘要 The safety and toxicity of carbon nanomaterials has recently become a major debate despite that the structural and electrical properties, in addition to the wide range of functionalization, had lent themselves to a variety of biomedical applications. Among the members in the carbon nanomaterials family, carbon nanocapsules (CNCs) may be the better candidate for biomedical use due to their delicate size and sophisticated structure. In this thesis, I report the preparation of CNCs using different surfactants and examine their in vitro and in vivo biocompatibility comprehensively and systemically. Polyvinyl alcohol (PVA)-stabilized CNCs have the lowest cytotoxicity to cultured stem cells, cancer cells and fibroblasts, and cause no obvious immunoresponse in human monocytes. Following intravenous injection in mice, no significant systemic inflammatory reaction was observed. Together these results suggest that CNCs are biocompatible nanomaterials and thus may be useful for biomedical applications.
論文目次 Abstract in English 1
Abstract in Chinese 2
Acknowledgement 3
Table of Contents 4
Index of figures 6

1. Introduction 7
1.1 Nanotechinology and the toxicity of nanoparticles 7
1.2 Carbon nanomaterials and carbon nanocapsules 7
1.3 The safety of carbon nanomaterials 8

2. Materials and Methods 10
2.1 Preparation of different surfactant stabilized CNCs solutions 10
2.2 Cell culture and in vitro cell viability and apoptosis assays 10
2.3 In vitro immunoresponse study by human monocyte cell lines (U937) 12
2.4 Animal experiments and in vivo biocompatibility of CNCs 13
2.5 Statistic analysis 15

3. Results and discussions 16
3.1 Preparation of water-soluble CNCs by surfactant stabilization 16
3.2 In vitro cytotoxicity of different surfactant stabilized CNCs 20
3.3 In vitro immunoresponse of different surfactant stabilized CNCs 28
3.4 In vivo biocompatibility of PVA stabilized CNCs 32

4. Conclusion 43
Reference 45
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