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系統識別號 U0026-0609201022573600
論文名稱(中文) 探討大鼠纖維肌痛症模型之睡眠及似焦慮/憂鬱行為
論文名稱(英文) Sleep and anxiety- / depression-like behaviors in a rat model of fibromyalgia
校院名稱 成功大學
系所名稱(中) 認知科學研究所
系所名稱(英) Institute of Cognitive Science
學年度 98
學期 2
出版年 99
研究生(中文) 邵彥文
研究生(英文) Yen-Wen Shao
學號 U7696103
學位類別 碩士
語文別 中文
論文頁數 52頁
口試委員 指導教授-蕭富仁
口試委員-嚴震東
口試委員-徐百川
口試委員-蔡玲玲
中文關鍵字 纖維肌痛症  痛覺過敏  睡眠障礙  淺睡期  深睡期  焦慮  憂鬱 
英文關鍵字 Fibromyalgia  Hyperalgesia  sleep disorder  NREM1  NREM2  anxiety  depression 
學科別分類
中文摘要 纖維肌痛症患者長期受苦於全身性肌肉疼痛而嚴重影響生活作息,臨床研究指出纖維肌痛症患者常伴隨淺睡增加之睡眠障礙及焦慮、憂鬱等情緒問題。目前臨床上尚無確切有效治療纖維肌痛症的方式,主要原因可能與纖維肌痛症病理機轉尚不清楚有關,因此Sluka(2001)提出於大鼠腓腸肌重複施打pH4.0溶液以誘發大鼠雙側腳掌長期性的痛覺過敏現象,為了驗證此動物模型的實用性,本研究進一步檢驗此動物模型的睡眠及焦慮、憂鬱表現是否跟纖維肌痛症患者有類似的表現。研究結果顯示,大鼠被誘發疼痛後,淺睡期時數顯著增加,腳掌退縮閾值與淺睡期時數呈負相關、而與深睡期時數呈正相關。在大鼠被誘發疼痛後可記錄到進入架高型十字迷宮的開放式空間時間顯著減短,且進入開放空間之中央區域時間也縮短,糖水之攝取量顯著減少,但強迫游泳測試中不動時間則沒有差異。本研究結果證實以酸性溶液誘發的大鼠纖維肌痛症模型會有與疼痛嚴重度相關之淺睡增加現象以及高焦慮且喪失興趣之憂鬱行為產生,這些現象支持利用酸性溶液誘發長期疼痛方式適合做為纖維肌痛症之動物模型。
英文摘要 Fibromyalgia patients suffer from chronic widespread muscle pain. Increased light sleep and anxiety/depression disorders are major comorbidities of fibromyalgia. To date, no effective treatment on fibromyalgia in clinic may be due to poor understanding of its mechanism. Sluka (2001) introduced an animal model with induced bilateral, long-lasting hyperalgesia by repeatedly injecting pH4.0 saline into left gastrocnemius muscle to mimic fibromyalgia. To further valid the fibromyalgia model, recordings of sleep and anxiety/depression behaviors were carried out. First phase of non-rapid eye movement (NREM) sleep was significantly increased with a negative trend with paw withdrawal threshold when pain occurred. Moreover, a significant positive relationship was found between paw withdrawal threshold and duration of second phase of NREM sleep, i.e., slow-wave sleep. Hyperalgesic rats displayed significantly longer duration entering open arm of an elevated plus maze and remarkably longer duration crossing central zone of an open field. They also showed anhedonic behavior on the sucrose consumption. However, there was no difference in immobility of forced swimming test. According to our results, acid-induced hyperalgesic rats display pain-related elevation on duration of light sleep, anxious behavior, and anhedonic depressive behavior. Thus, our data suggest that the acid-induced pain is an appropriate model of fibromyalgia.
論文目次 摘要 I
Abstract II
誌謝 IV
目錄 V
圖目錄 VII
符號 IX
緒論 1
慢性疼痛 1
纖維肌痛症 1
焦慮與憂鬱 2
睡眠障礙 3
大鼠纖維肌痛症模型 4
研究目的與實驗假設 5
研究方法 6
實驗動物 6
實驗設計 6
實驗一 6
誘發疼痛及疼痛反應測量 6
體重測量 7
似焦慮/憂鬱行為測量 8
實驗二 10
手術 10
訊號記錄及分析 11
資料分析 12
研究結果 13
誘發疼痛結果 13
似焦慮/憂鬱行為結果 14
睡眠結果 16
討論 21
結論 23
參考文獻 47
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