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系統識別號 U0026-0602201812083300
論文名稱(中文) 厭氧系統中甲烷菌與乙酸菌競爭關係之研究
論文名稱(英文) Interaction and Competition Between Methanogens and Acetogens in Anaerobic System
校院名稱 成功大學
系所名稱(中) 環境工程學系
系所名稱(英) Department of Environmental Engineering
學年度 106
學期 1
出版年 107
研究生(中文) 陳威威
研究生(英文) Michael Setiadi Tanera
學號 P56057032
學位類別 碩士
語文別 英文
論文頁數 93頁
口試委員 口試委員-郭獻文
口試委員-童心欣
指導教授-黃良銘
中文關鍵字 none 
英文關鍵字 acetogens  ampicillin  inhibition  methanogens  2-bromoethanesulfonate 
學科別分類
中文摘要 none
英文摘要 Nowadays, energy demand is one of the hottest issue that we have. Caused by human population exponential growth in last decades, energy is predicted would not enough for fulfilling human needs, but higher bio-waste. Anaerobic bacteria have ability to convert common bio-waste such as polysaccharide to make a methane gas which has a big energy storage. In this research, we focus on the last step of methane production anaerobic bacteria, which are methanogens and acetogens. We examine the effect of acetogens and methanogens inhibition in anaerobic system. We use 2-bromoethanesulfonate (BES) and ampicillin for doing inhibition on methanogens and acetogens, respectively. Batch culture has done in different initial condition to analyze their performance at 35oC temperature and on 120 rpm shaker.
Results shows that 50 mM of BES can fully inhibit 5 mL of sludge in 150 mL working volume, meanwhile 300 ppm of ampicillin can inhibit acetogens. Moreover, from the result we can conclude that methanogens will produce methane gas when the hydrogen gas concentration inside the system is low. Hydrogenotrophic methanogens and acetoclastic methanogens will have better performance when each of them work separately. Thus, the higher acetate concentration will generate acetoclastic methanogens to increase the ratio between methane and carbon dioxide production. Acetoclastic methanogens and hydrogenotrophic methanogens has 48 hours and 72 hours of lag phase, respectively, before they produce methane gas. Nevertheless, Acetoclastic methanogens has higher rate than Hydrogenotrophic methanogens, but after around 2.5 mmol x carbon atom of substrate is exist, the Hydrogenotrophic methanogens has higher methane production rate.
論文目次 ABSTRACT i
ACKNOWLEDGEMENT ii
TABLE OF CONTENTS iv
LIST OF TABLES viii
LIST OF FIGURES ix
CHAPTER 1 INTRODUCTION 1
1.1 Background 1
1.2 Objectives of Study 3
CHAPTER 2 LITERATURE REVIEW 4
2.1 World Energy Demand and The Problem 4
2.3 Methane Combustion as Source of Renewable Energy 6
2.2 Anaerobic Digestion 9
2.4 Methanogens 10
2.4.1 Overview of Methanogens 10
2.4.2 Methanogens Order 11
2.4.3 Methanogens Ecology 15
2.4.4 Methanogens Metabolic Pathway 27
2.5 Acetogens 33
2.5.1 Overview of Acetogens 33
2.5.2 Acetogens Habitat 37
2.5.3 Acetogens Metabolic Pathway 37
2.6 Effect of Inhibitor Compound to Methanogens Bacteria 41
2.6.1 Structural Analog of Co-Enzymes A 42
2.6.2 Hydroxymethylglutaryl-SCoA (HMG-CoA) reductase 43
2.6.3 Medium or Long Chain Fatty Acid 44
2.6.4 Nitrocompound and Phosphate 44
2.6.5 Ethylene and Acetylene 45
2.6.6 Halogenated Aliphatic Hydrocarbon 46
2.7 Effect of Inhibitor Antibiotics to Acetogens Bacteria 46
2.7.1 Ampicillin 46
2.7.2 Florfenicol 47
2.7.3 Tylosin 47
CHAPTER 3 METHODOLOGY 49
3.1 The Approach of This Work 49
3.2 Medium Preparation 52
3.3 Sludge Preparation 53
3.4 Continuous Stirred Tank Reactor 54
3.5 Batch Culture 55
3.5.1 Batch A 56
3.5.2 Batch B 57
3.5.3 Batch C 57
3.5.4 Batch D 58
3.5.5 Batch E 58
3.5.6 Batch F 59
3.5.7 Batch G 59
3.5.8 Batch H 60
3.6 Analytical Method 61
3.6.1 Gas Analysis 61
3.6.2 HPLC Analysis 61
CHAPTER 4 RESULT AND DISCUSSION 62
4.1 Batch A as Control 63
4.2 BES Concentration to Inhibit Methanogens 65
4.3 Ampicillin Concentration Inhibit Acetogens 68
4.4 Glucose Effect in Anaerobic Sludge 70
4.5 Homoacetogens Performance 74
4.6 Acetoclastic Methanogens Performance 75
4.7 Hydrogenotrophic Methanogens Performance 79
4.8 Glucose Consumption without Methanogens 82
4.9 Methane Production Rate Comparison 84
4.10 The Effects of Glucose in Homoacetogens Performance 85
CHAPTER 5 CONCLUSION 87
REFERENCES 89
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