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系統識別號 U0026-0301201715333300
論文名稱(中文) 探討鬥魚的侵略行為與差異表現基因分析
論文名稱(英文) Characterization of Aggressive Behavior and Analysis of Differential Gene Expression Involved in Fighting Fish (Betta splendens)
校院名稱 成功大學
系所名稱(中) 生命科學系
系所名稱(英) Department of Life Sciences
學年度 105
學期 1
出版年 106
研究生(中文) 武趙德
研究生(英文) Vu, Trieu-Duc
學號 L56047050
學位類別 碩士
語文別 英文
論文頁數 76頁
口試委員 指導教授-岡田典弘
指導教授-蔣鎮宇
口試委員-江友中
口試委員-黃浩仁
中文關鍵字 none 
英文關鍵字 Betta splendens  Aggressive Behaviour  Differential Expression Genes  Cognitive Disorder 
學科別分類
中文摘要 none
英文摘要 There is increasing appreciation that social experience can alter brain gene expression and behavior. Previous studies showed that social experience leads to changes in brain gene expression, brain function, and social behavior. However, relatively there are still few studies that have examined complex behaviors and correlated them with gene expression in brain. Here I use fighting fish Betta splendens to characterize the dynamic structure of the fight in male dyads and correlate the fight outcomes with gene expression changes on brains of winner and loser at both behavioral and molecular levels. As the results of behavior analysis, I discovered that mouth-locking behavior is the most critical among all the behaviors, because other behavior (e.g, bite & strike and surface-breathing) are restricted during mouth-locking duration in male dyads. Probably, due to this restriction, behavior of opponents in a fight pair synchronize with each other in both the behavioral and transcriptome analysis. Furthermore, the data of differential expression genes (DEGs) showed that all individuals from each social treatment were grouped together representing that the brain gene expression of winner and loser reflect their recent social experiences. Besides, we found that there are only 4 genes expressed differentially between winner and loser at just after fighting (End0), but 30 min later (End30) 29 genes were differentiated (FDR < 0.05), showing that it needs to take some time for the internal response of winner and loser to be distinct. In addition, the expression patterns of some candidate genes for cognitive disorder were investigated, and 23 autism associated genes were shown to belong to the group exhibiting low expression in loser. This study provides a cornerstone for understanding of fighting itself in molecular level, and how it can be used to identify of genes underlying specific behaviors and mechanisms involved in neuropathogeneis.
論文目次 1. INTRODUCTION 1
1.1. Aggressive behavior background 1
1.2. Aggression in behavioral level 2
1.3. Aggression relates to brain regions 4
1.4. Aggression in physiological and hormonal mechanisms level 6
1.5. Correlated complex behaviors to brain gene expression 7
1.6. Aggression in the context combined molecular genetics and behavior 9
1.7. Objective of Study 10
1.8. The limitation of study 10
1.9. Organization of the Report 10
1. MATERIALS AND METHODS 12
2.1. Subject and maintenance. 12
2.2. Behavioral Assays 12
2.3. Behavioral analysis 13
2.4. Tissue Processing, RNA extraction. 13
2.5. Generated RNA-Seq Data. 14
2.6. Gene Expression Analysis 14
3. RESULTS 17
3.1. Aggressive behavior 17
3.1.1. Dynamic structure of the fight 17
3.1.2. Aggressiveness profiles winner and loser 18
3.1.3. Behavioral patterns of winner and loser 18
3.2. Differential Expression Gene Analysis 20
3.3. Candidate genes in the context of cognitive disorders 23
4. DISCUSSION 24
4.1. Fighting Duration 24
4.2. Fighting phases 25
4.3. First given behavior 26
4.4. Behavior patterns of winner and loser 26
4.5. Genes and behavior 27
5. CONCLUSION AND SUGGESTIONS 29
5.1. Conclusions 29
5.2. Recommendations 30
5.3. Area for further study 30
References 32
Appendix 1: Tables 39
Appendix 2: Figures 69
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