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系統識別號 U0026-0301201316474000
論文名稱(中文) Annexin A1惡化單純疱疹病毒第一型感染小鼠的失明性角膜炎
論文名稱(英文) Annexin A1 Aggravates Blinding Herpetic Stromal Keratitis in Mice
校院名稱 成功大學
系所名稱(中) 微生物及免疫學研究所
系所名稱(英) Department of Microbiology & Immunology
學年度 98
學期 1
出版年 98
研究生(中文) 黃偉誠
研究生(英文) Wei-Chen Huang
學號 nckutest119
學位類別 碩士
語文別 中文
論文頁數 36頁
口試委員 指導教授-陳舜華
口試委員-張堯
口試委員-王憲威
中文關鍵字 單純疱疹病毒第一型  疱疹性失明性角膜炎  Annexin A1 
英文關鍵字 Annexin A1  Herpetic Stromal Keratitis  HSV-1 
學科別分類
中文摘要 單純疱疹病毒第一型(herpes simplex virus type 1)所誘導的疱疹性失明性角膜炎(herpetic stromal keratitis)肇因於復發的單純疱疹病毒在眼部組織生長所引起的慢性發炎疾病,並且在病患眼睛中伴隨著嚴重的細胞浸潤及血管新生現象。疱疹性失明性角膜炎是目前已知最常導致視覺障礙和失明的感染症,在先前研究指出宿主的細胞因子會調控疱疹性失明性角膜炎的生成,然而到底有那些細胞因子會幫助病毒複製目前仍不清楚。先前一篇研究中,發現在一個缺乏annexin A1(Anx-A1)的細胞株不會被單純疱疹病毒第一型感染。目前在單純疱疹病毒第一型的研究中,Anx-A1的功能尚未被探討過,因此本篇我們利用小鼠模式來探討Anx-A1在單純疱疹病毒第一型所誘導的失明性角膜炎中所扮演的角色。根據我們在小鼠胚胎纖維母細胞研究結果顯示,被感染細胞的Anx-A1表現量會增加。此外,我們也發現Anx-A1缺陷型的小鼠胚胎纖維母細胞在感染後所產生的病毒量明顯比野生型的細胞低。另外,實驗結果也顯示在利用相同的病毒量進行感染時,可被病毒感染的Anx-A1缺陷型細胞數比野生型細胞少。在小鼠的失明性角膜炎模式中,我們發現單純疱疹病毒第一型在感染Anx-A1缺陷型的小鼠後所誘導的失明性角膜炎的嚴重程度以及在小鼠眼睛中的病毒量都有顯著的降低。綜合以上實驗結果,我們發現Anx-A1會惡化單純疱疹病毒第一型感染小鼠所誘導的失明性角膜炎。
英文摘要 Herpes simplex virus (HSV) infects eyes and causes herpetic stromal keratitis (HSK), a chronic inflammatory disease with excessive neovascularization in corneas. HSK has been the most common cause of corneal blindness in the western world due to infection. Host factors mediate HSK development, but little is known about these cellular factors and their mechanisms. A cell clone of rat intestine epithelial cells with mutation in annexin A1 (Anx-A1) gene is shown to be resistant to HSV-1 infection with unknown mechanisms. To date, the functions of Anx-A1 in HSK remain unclear. In the present study, we used a mouse model to investigate the roles of Anx-A1 in HSK. In vitro studies showed that Anx-A1 expression was enhanced after HSV-1 infection in mouse embryonic fibroblasts. In addition, the amount of virus produced by mouse embryonic fibroblasts cultured from Anx-A1-deficient mice was significantly lower than that produced by mouse embryonic fibroblasts from wild-type mice. Moreover, less mouse embryonic fibroblasts cultured from Anx-A1- deficient mice were infected by HSV-1 compared to those cultured from wild-type mice. In vivo studies demonstrated that the severity of HSK and viral load in the eyes of Anx-A1-deficient mice were significantly reduced compared with those of wild-type mice. Collectively, our results show that Anx-A1 aggravates HSK in mice.
論文目次 中文摘要…………………………………………………………3
英文摘要…………………………………………………………4
致謝………………………………………………………………5
目錄………………………………………………………………7
圖次………………………………………………………………8
緒論………………………………………………………………9
實驗材料與方法…………………………………………………13
結果………………………………………………………………17
討論………………………………………………………………22
參考文獻…………………………………………………………25
附圖………………………………………………………………29
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